{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:25:43Z","timestamp":1772159143267,"version":"3.50.1"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T00:00:00Z","timestamp":1726185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["SCHW382\/17-1"],"award-info":[{"award-number":["SCHW382\/17-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005707","name":"Akademie f\u00fcr Tiergesundheit","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005707","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To analyse porcine Pasteurella multocida isolates obtained from the national resistance monitoring program GERM-Vet 2008\u20132021 in Germany for phenotypic and genotypic macrolide resistance.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The antimicrobial susceptibility of the isolates was determined by broth microdilution according to CLSI standards. Closed genomes were obtained by WGS via Illumina MiSeq and MinION platforms followed by a hybrid assembly.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Of 1114 porcine P. multocida isolates, only four isolates (0.36%), one each from 2008, 2010, 2019 and 2021, exhibited resistance to at least one macrolide tested. The isolate from 2010 was only resistant to erythromycin and WGS analysis neither revealed a macrolide resistance gene nor a macrolide resistance-mediating mutation. The isolates from 2008 and 2019 were resistant to erythromycin, tilmicosin, tildipirosin, tulathromycin and gamithromycin and showed either only the A2058G mutation in all six 23S rRNA operons or the chromosomally located macrolide resistance genes msr(E) and mph(E), respectively. The isolate from 2021 was resistant to erythromycin, tulathromycin, gamithromycin and tylosin and carried a novel integrative and conjugative element of 64\u200a966\u2005bp, designated Tn7730, in its chromosomal DNA. It harboured the macrolide resistance genes mef(C), mph(G) and estT, the lincosamide resistance gene lnu(H), and the tetracycline resistance gene tet(Y), the last two were detected for the first time in P. multocida.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Macrolide resistance in German porcine P. multocida can be due to resistance-mediating mutations or resistance genes. The presence of the novel Tn7730 carrying three different macrolide resistance genes is alarming and should be monitored.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkae316","type":"journal-article","created":{"date-parts":[[2024,9,14]],"date-time":"2024-09-14T08:14:29Z","timestamp":1726301669000},"page":"2975-2979","source":"Crossref","is-referenced-by-count":4,"title":["Genetic basis of macrolide resistance in porcine <i>Pasteurella multocida<\/i> isolates from the German national resistance monitoring program GE<i>RM<\/i>-Vet 2008\u20132021"],"prefix":"10.1093","volume":"79","author":[{"given":"Henrike","family":"Kr\u00fcger-Haker","sequence":"first","affiliation":[{"name":"Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin ,","place":["Germany"]},{"name":"Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Kostova","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin ,","place":["Germany"]},{"name":"Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis","family":"Hanke","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin ,","place":["Germany"]},{"name":"Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heike","family":"Kaspar","sequence":"additional","affiliation":[{"name":"Federal Office of Consumer Protection and Food Safety (BVL) , Department Method Standardisation, Reference Laboratories, Resistance to Antibiotics, Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Fiedler","sequence":"additional","affiliation":[{"name":"Federal Office of Consumer Protection and Food Safety (BVL) , Department Method Standardisation, Reference Laboratories, Resistance to Antibiotics, Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6303-8212","authenticated-orcid":false,"given":"Stefan","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin ,","place":["Germany"]},{"name":"Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,9,13]]},"reference":[{"key":"2024110323533103000_dkae316-B1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.ARBA-0022-2017","article-title":"Antimicrobial resistance in Pasteurellaceae of veterinary origin","volume":"6","author":"Michael","year":"2018","journal-title":"Microbiol Spectr"},{"key":"2024110323533103000_dkae316-B2","volume-title":"Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. 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