{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T15:54:31Z","timestamp":1781970871370,"version":"3.54.5"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Treatment of chronic hepatitis B infection requires lifelong administration of nucleos(t)ide analogues with a high barrier to resistance and effective viral suppression. The major limitation of lifelong therapy is the possible selection of drug-resistant hepatitis B virus (HBV) strains. International Liver Society guidelines recommend that hepatitis B resistance testing must be performed by a reference laboratory.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Performance of the deep sequencing-based ViroKey\u00ae SQ FLEX Genotyping Assay for the determination of HBV genotypes and resistance profiles were evaluated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Patients and methods<\/jats:title>\n                  <jats:p>Plasma samples collected from patients with chronic hepatitis B have been sequenced by two methods including Sanger (population) sequencing of a portion of the P\/S overlapping gene and the deep sequencing-based ViroKey\u00ae SQ FLEX Genotyping Assay (Vela Diagnostics).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>A high concordance rate with population sequencing was found regardless of HBV genotypes. Deep sequencing with the Sentosa platform was more sensitive than population sequencing in detecting minor variant populations.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The deep sequencing-based ViroKey\u00ae SQ FLEX Genotyping Assay can be confidently used in clinical practice for hepatitis B genotyping and resistance testing.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkae418","type":"journal-article","created":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T04:17:15Z","timestamp":1730434635000},"page":"919-926","source":"Crossref","is-referenced-by-count":2,"title":["Performance evaluation of a fully automated deep sequencing platform for hepatitis B genotyping and resistance testing"],"prefix":"10.1093","volume":"80","author":[{"given":"Val\u00e9rie","family":"Ortonne","sequence":"first","affiliation":[{"name":"Department of Virology, French National 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