{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:53:04Z","timestamp":1791561184310,"version":"4.3.4"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T00:00:00Z","timestamp":1731628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"UKHSA"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>In England, group B streptococci (GBS; Streptococcus agalactiae) are considered universally susceptible to penicillin. Reports from Africa, Asia, North America and a few European countries have described GBS isolates with penicillin MICs above the epidemiological cut-off (0.125 mg\/L). Our aim was to characterize a penicillin-resistant GBS (PRGBS) isolate recovered in 2016 from a patient treated with long-term antimicrobials in the UK.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Antibiotic susceptibility of a referred isolate from a discharging sinus overlying a chronic prosthetic joint infection was determined using gradient strip testing for seven antibiotics. Illumina short read sequencing was carried out using a HiSeq 2500 platform to determine MLST, capsular type, to detect mutations in the pbp genes, and to compare the isolate with contemporaneous GBS isolates circulating in the UK.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The GBS isolate belonged to capsular type Ia and MLST 144. We observed resistance to penicillin (MIC\u200a=\u200a1 mg\/L) and tetracycline (32 mg\/L) with susceptibility to linezolid (1 mg\/L), erythromycin (0.064 mg\/L), clindamycin (0.064 mg\/L), teicoplanin (0.064 mg\/L) and vancomycin (0.25 mg\/L). Deduced amino acid sequences revealed substitutions and non-synonymous changes in PBP2x and PBP2b. Genomic analysis of contemporaneous cases (n\u200a=\u200a34) from across the UK identified single nucleotide polymorphism (SNP) variation ranged from 153\u20136596 SNPs.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We confirm the first identification of a PRGBS isolate amongst referrals to the UK\u2019s national reference laboratory. Substitutions in pbp1a, pbp2a, pbp2x and pbp2b were identified that likely developed in the face of long-term beta-lactam antibiotic use.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkae419","type":"journal-article","created":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T11:26:08Z","timestamp":1731669968000},"page":"399-404","source":"Crossref","is-referenced-by-count":21,"title":["A case of penicillin-resistant group B <i>Streptococcus<\/i> isolated from a patient in the UK"],"prefix":"10.1093","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7097-4116","authenticated-orcid":false,"given":"E","family":"McGuire","sequence":"first","affiliation":[{"name":"Healthcare-Associated Infection (HCAI), Fungal, Antimicrobial Resistance (AMR), Antimicrobial Use (AMU), and Sepsis Division, United Kingdom Health Security Agency (UKHSA) , London 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,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Potterill","sequence":"additional","affiliation":[{"name":"Healthcare-Associated Infection (HCAI), Fungal, Antimicrobial Resistance (AMR), Antimicrobial Use (AMU), and Sepsis Division, United Kingdom Health Security Agency (UKHSA) , London ,","place":["UK"]},{"name":"Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, UKHSA , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Pike","sequence":"additional","affiliation":[{"name":"Healthcare-Associated Infection (HCAI), Fungal, Antimicrobial Resistance (AMR), Antimicrobial Use (AMU), and Sepsis Division, United Kingdom Health Security Agency (UKHSA) , London ,","place":["UK"]},{"name":"Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, UKHSA , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7279-5322","authenticated-orcid":false,"given":"K L","family":"Hopkins","sequence":"additional","affiliation":[{"name":"Healthcare-Associated Infection (HCAI), Fungal, Antimicrobial Resistance (AMR), Antimicrobial Use (AMU), and Sepsis Division, United Kingdom Health Security Agency (UKHSA) , London ,","place":["UK"]},{"name":"Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, UKHSA , London ,","place":["UK"]},{"name":"NIHR Health Protection Research Unit in Healthcare Associated Infections and Antimicrobial Resistance, Imperial College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5500-5691","authenticated-orcid":false,"given":"R L","family":"Guy","sequence":"additional","affiliation":[{"name":"Healthcare-Associated Infection (HCAI), Fungal, Antimicrobial Resistance (AMR), Antimicrobial Use (AMU), and Sepsis Division, United Kingdom Health Security Agency (UKHSA) , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Lamagni","sequence":"additional","affiliation":[{"name":"Healthcare-Associated Infection (HCAI), Fungal, Antimicrobial Resistance (AMR), Antimicrobial Use (AMU), and Sepsis Division, United Kingdom Health Security Agency (UKHSA) , London ,","place":["UK"]},{"name":"NIHR Health Protection Research Unit in Healthcare Associated Infections and Antimicrobial Resistance, Imperial College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Mack","sequence":"additional","affiliation":[{"name":"Division of Infection, Immunity and Rare Diseases, Royal Free London NHS Foundation Trust , London ,","place":["UK"]},{"name":"Bone Infection Unit, Royal National Orthopaedic Hospital , Stanmore ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Scobie","sequence":"additional","affiliation":[{"name":"Division of Infection, Immunity and Rare Diseases, Royal Free London NHS Foundation Trust , London ,","place":["UK"]},{"name":"Bone Infection Unit, Royal National Orthopaedic Hospital , Stanmore ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Warren","sequence":"additional","affiliation":[{"name":"Division of Infection, Immunity and Rare Diseases, Royal Free London NHS Foundation Trust , London ,","place":["UK"]},{"name":"Bone Infection Unit, Royal National Orthopaedic Hospital , Stanmore ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C S","family":"Brown","sequence":"additional","affiliation":[{"name":"Healthcare-Associated Infection (HCAI), Fungal, Antimicrobial Resistance (AMR), Antimicrobial Use (AMU), and Sepsis Division, United Kingdom Health Security 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