{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:36:10Z","timestamp":1790246170068,"version":"4.1.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Australian Institute for Microbiology"},{"name":"United States Defense Health Program"},{"name":"Australian Government Research Training Program"},{"DOI":"10.13039\/501100020930","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020930","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To study the population structure and genomic characteristics, including antimicrobial resistance genes, plasmid types and surface polysaccharide type, of the globally distributed Acinetobacter baumannii belonging to ST32 (Institut Pasteur scheme).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Antibiotic resistance phenotype for 19 antibiotics was determined using Vitek 2. Whole-genome sequencing was performed using the Illumina MiSeq platform. Genomes were assembled using Newbler. Phylogenetic analysis was done by determining the core-genome alignments using Panaroo v1.3, analysed in IQ-Tree2 v2.2.0.3 to construct Maximum Likelihood trees using the RaxML software. Resistance genes and IS were identified using the Abricate programme, and ISFinder databases.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>One hundred and thirty-three (n\u200a=\u200a133) ST32 A. baumannii isolates were analysed in this study. These genomes originated mainly from US military treatment facilities (n\u200a=\u200a113), but also included additional publicly available genomes in GenBank (n\u200a=\u200a20) recovered from a broad geographic distribution extending to Asia and South America. Phylogenetic analysis of all 133 genomes revealed at least four clades, with over 80 genomes forming a tightly clustered branch, suggesting they are likely to represent outbreak strains. Analysis of the ampC region showed that ST32 strains played a significant role in the formation of the widely distributed ampC transposon, Tn6168, and supplying DNA segments containing an ISAba1-ampC from ST32s via homologous recombination.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>ST32 strains played a significant role in the evolution of antibiotic resistance in several widely distributed sequence types including ST1 (global clone 1) and ST3.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkae454","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T16:27:46Z","timestamp":1734366466000},"page":"666-675","source":"Crossref","is-referenced-by-count":6,"title":["Genomic analysis of early ST32 <i>Acinetobacter baumannii<\/i> strains recovered in US military treatment facilities reveals distinct lineages and links to the origins of the Tn<i>6168 ampC<\/i> transposon"],"prefix":"10.1093","volume":"80","author":[{"given":"Liam A","family":"Tobin","sequence":"first","affiliation":[{"name":"Australian Institute for Microbiology and Infection, University of Technology Sydney , Ultimo, NSW 2007 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eradah","family":"Abu Sabah","sequence":"additional","affiliation":[{"name":"Australian Institute for Microbiology and Infection, University of Technology Sydney , Ultimo, NSW 2007 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Lebreton","sequence":"additional","affiliation":[{"name":"Multidrug Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research , Silver Spring, MD ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garry S A","family":"Myers","sequence":"additional","affiliation":[{"name":"Australian Institute for Microbiology and Infection, University of Technology Sydney , Ultimo, NSW 2007 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1548-9438","authenticated-orcid":false,"given":"Patrick T","family":"McGann","sequence":"additional","affiliation":[{"name":"Multidrug Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research , Silver Spring, MD ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3614-7261","authenticated-orcid":false,"given":"Mehrad","family":"Hamidian","sequence":"additional","affiliation":[{"name":"Australian Institute for Microbiology and Infection, University of Technology Sydney , Ultimo, NSW 2007 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