{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T20:23:46Z","timestamp":1788639826159,"version":"build-2803163510"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013281","name":"Joint Programming Initiative on Antimicrobial Resistance","doi-asserted-by":"publisher","award":["JPIAMR_2021_P010"],"award-info":[{"award-number":["JPIAMR_2021_P010"]}],"id":[{"id":"10.13039\/100013281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"publisher","award":["ERP-2021-23680703"],"award-info":[{"award-number":["ERP-2021-23680703"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"Netherlands Organization for Health Research and Development ZonMw","doi-asserted-by":"publisher","award":["1070132110001"],"award-info":[{"award-number":["1070132110001"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["333858"],"award-info":[{"award-number":["333858"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"publisher","award":["ANR-21-AAMR-0004-02"],"award-info":[{"award-number":["ANR-21-AAMR-0004-02"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Polish National Science Centre NCN","award":["2021\/03\/Y\/NZ7\/00136"],"award-info":[{"award-number":["2021\/03\/Y\/NZ7\/00136"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To establish epidemiological cut-off values (ECOFFs) for the polyether ionophores narasin, salinomycin, lasalocid and monensin in Enterococcus faecium.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>MICs were measured using the broth microdilution method according to ISO 20776-1 (2019). Method validation involved \u226510 replicates of antimicrobial susceptibility testing of Enterococcus faecalis ATCC 29212. A total of 182 E. faecium isolates from various sources were tested in five European laboratories. The ECOFFinder tool from EUCAST was used to establish the ECOFFs for 122 WT isolates, verified by PCR or WGS.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Method validation showed consistency, with acceptable variation within \u00b11 2-fold dilution. The ECOFF for narasin was 0.5\u2005mg\/L, considerably below the current EUCAST ECOFF for E. faecium (ECOFF\u200a=\u200a2\u2005mg\/L). Salinomycin and lasalocid ECOFFs were 1 and 2\u2005mg\/L, respectively. Strains carrying the previously identified narAB resistance genes clearly manifested a separate MIC distribution for narasin and salinomycin, but not for lasalocid, although a clear bias to the higher MIC values within the normal distribution could be observed. Monensin apparently displayed a broader MIC range (0.5\u201364\u2005mg\/L) with multiple modes, which precluded the establishment of an ECOFF for monensin.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The study yielded novel ECOFFs for distinguishing WT E. faecium strains for the key veterinary ionophores, providing a mainstay for a better understanding of ionophore resistance in enterococci.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaf113","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T08:12:49Z","timestamp":1753949569000},"page":"2361-2368","source":"Crossref","is-referenced-by-count":2,"title":["Determination of epidemiological cut-off values for Narasin, Salinomycin, Lasalocid and Monensin in <i>Enterococcus faecium<\/i>"],"prefix":"10.1093","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1398-8244","authenticated-orcid":false,"given":"Maria Laura","family":"Ferrando","sequence":"first","affiliation":[{"name":"Division Immunology, Transplant and Infectious Diseases, IRCCS Ospedale San Raffaele (OSR) , Via Olgettina 60, Milan 20123 ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6705-1716","authenticated-orcid":false,"given":"Francesca","family":"Saluzzo","sequence":"additional","affiliation":[{"name":"Division Immunology, Transplant and Infectious Diseases, IRCCS Ospedale San Raffaele (OSR) , Via Olgettina 60, Milan 20123 ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikki Franklin","family":"Frederiksen","sequence":"additional","affiliation":[{"name":"Department of Animal Health and Food Safety, Norwegian Veterinary Institute (NVI) , P.O. Box 64, \u00c5s 1431 ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jannice Schau","family":"Sletteme\u00e5s","sequence":"additional","affiliation":[{"name":"Department of Animal Health and Food Safety, Norwegian Veterinary Institute (NVI) , P.O. Box 64, \u00c5s 1431 ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Margrete","family":"Urdahl","sequence":"additional","affiliation":[{"name":"Department of Animal Health and Food Safety, Norwegian Veterinary Institute (NVI) , P.O. Box 64, \u00c5s 1431 ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Simm","sequence":"additional","affiliation":[{"name":"Department of Biosciences, University of Oslo , P.O. Box 1066, Blindern, Oslo 0316, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Skar\u017cy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Veterinary Research Institute (PIWet) , Partyzantow Avenue 57, Pulawy 24-100 ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8155-7614","authenticated-orcid":false,"given":"Magdalena","family":"Zaj\u0105c","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Veterinary Research Institute (PIWet) , Partyzantow Avenue 57, Pulawy 24-100 ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Lalak","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Veterinary Research Institute (PIWet) , Partyzantow Avenue 57, Pulawy 24-100 ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jowita","family":"Niczyporuk","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Veterinary Research Institute (PIWet) , Partyzantow Avenue 57, Pulawy 24-100 ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dariusz","family":"Wasyl","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Veterinary Research Institute (PIWet) , Partyzantow Avenue 57, Pulawy 24-100 ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9a","family":"Duval","sequence":"additional","affiliation":[{"name":"ANSES, Ploufragan, Plouzan\u00e9 Niort laboratory, Mycoplasmology Bacteriology and Antimicrobial Unit (ANSES), Zoopole Les Croix , BP 53, Ploufragan 22440 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Tardy","sequence":"additional","affiliation":[{"name":"ANSES, Ploufragan, Plouzan\u00e9 Niort laboratory, Mycoplasmology Bacteriology and Antimicrobial Unit (ANSES), Zoopole Les Croix , BP 53, Ploufragan 22440 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Schapendonk","sequence":"additional","affiliation":[{"name":"Wageningen University and Research , Wageningen Food Safety Research (WFSR), P.O. 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