{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T09:10:03Z","timestamp":1751361003454,"version":"3.41.0"},"reference-count":7,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T00:00:00Z","timestamp":1748217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"German Ministry of Health"},{"name":"National Reference Center"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Mycobacterium haemophilum is a fastidious nontuberculous mycobacterium that causes severe infections in immunocompromised patients. Due to its complex growth requirements, its detection and antimicrobial susceptibility testing (AST) remain challenging. This study aimed to compare the performance of a modified commercial broth microdilution method (Sensititre\u2122 Myco susceptibility plates with added haemin) with the standard Clinical and Laboratory Standards Institute (CLSI) agar disk elution method for AST of M. haemophilum.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We conducted 20 comparative AST tests on M. haemophilum ATCC 29548 and 10 clinical isolates, using the CLSI agar disk elution method as the reference. MICs were determined using both methods, and categorical concordance was assessed for multiple antibiotics.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The overall concordance between methods was 81.4%. High agreement was observed for clarithromycin, linezolid, trimethoprim-sulfamethoxazole and rifampicin (90%\u2013100%), while lower concordance was noted for amikacin (40%), ciprofloxacin (75%) and doxycycline (65%). Sensititre\u2122 microdilution plates demonstrated superior standardization, ease of use and reduced contamination risks compared to the agar disk method.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The modified Sensititre\u2122 microdilution method is a reliable alternative to the agar disk elution method for AST of M. haemophilum. It provides precise MIC values and is better suited for routine clinical use due to improved reproducibility and efficiency.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaf162","type":"journal-article","created":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T07:27:41Z","timestamp":1748244461000},"page":"1993-1996","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of a modified broth microdilution Sensititre\u2122 SLOMYCOI assay with the CLSI-recommended agar disk elution method for antimicrobial susceptibility testing of <i>Mycobacterium haemophilum<\/i>"],"prefix":"10.1093","volume":"80","author":[{"given":"Doris","family":"Hillemann","sequence":"first","affiliation":[{"name":"Diagnostic Mycobacteriology, Research Center Borstel , Borstel ,","place":["Germany"]},{"name":"National and Supranational Reference Center for Mycobacteria, Research Center Borstel , Borstel ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuelle","family":"Cambau","sequence":"additional","affiliation":[{"name":"Associated Laboratory of the National Reference Center for Mycobacteria and Antimycobacterial Resistance, AP-HP GHU Nord, Mycobacteriology, Inserm, IAME UMR 1137, University of Paris Cite , Paris ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silke","family":"Polsfu\u00df","sequence":"additional","affiliation":[{"name":"Institute for Microbiology, Immunology and Laboratory Medicine, Medical Care Center at the Helios Klinikum Emil von Behring , Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inna","family":"Friesen","sequence":"additional","affiliation":[{"name":"Diagnostic Mycobacteriology, Research Center Borstel , Borstel,","place":["Germany"]},{"name":"National and Supranational Reference Center for Mycobacteria, Research Center Borstel , Borstel ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,5,26]]},"reference":[{"key":"2025070104564825900_dkaf162-B1","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1111\/j.1468-3083.2004.00781.x","article-title":"First case of Mycobacterium haemophilum infection in an AIDS patient in Italy","volume":"18","author":"Martinelli","year":"2004","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"2025070104564825900_dkaf162-B2","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1128\/jcm.11.2.190-192.1980","article-title":"Mycobacteria with a growth requirement for ferric ammonium citrate, identified as Mycobacterium haemophilum","volume":"11","author":"Dawson","year":"1980","journal-title":"J Clin Microbiol"},{"key":"2025070104564825900_dkaf162-B3","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1128\/CMR.00020-11","article-title":"Clinical manifestations, diagnosis, and treatment of Mycobacterium haemophilum infections","volume":"24","author":"Lindeboom","year":"2011","journal-title":"Clin Microbiol Rev"},{"key":"2025070104564825900_dkaf162-B4","doi-asserted-by":"publisher","first-page":"2323","DOI":"10.1128\/AAC.37.11.2323","article-title":"Activities of antimicrobial agents against clinical isolates of Mycobacterium haemophilum","volume":"37","author":"Bernard","year":"1993","journal-title":"Antimicrob Agents Chemother"},{"key":"2025070104564825900_dkaf162-B5","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1086\/321894","article-title":"Mycobacterium haemophilum in immunocompromised patients","volume":"33","author":"Shah","year":"2001","journal-title":"Clin Infect Dis"},{"key":"2025070104564825900_dkaf162-B6","first-page":"1","volume-title":"Manual of Clinical Microbiology. ClinMicroNow","author":"Brown-Elliott","year":"2023","edition":"1st ed"},{"author":"CLSI","key":"2025070104564825900_dkaf162-B7"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/80\/7\/1993\/63352705\/dkaf162.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/80\/7\/1993\/63352705\/dkaf162.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T08:56:54Z","timestamp":1751360214000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/80\/7\/1993\/8148914"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,26]]},"references-count":7,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,5,26]]},"published-print":{"date-parts":[[2025,7,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkaf162","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"type":"print","value":"0305-7453"},{"type":"electronic","value":"1460-2091"}],"subject":[],"published-other":{"date-parts":[[2025,7]]},"published":{"date-parts":[[2025,5,26]]}}}