{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:10:03Z","timestamp":1790665803152,"version":"4.1.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"National Institute for Health and Care Research Health Protection Research Unit"},{"name":"UK Health Security Agency"},{"DOI":"10.13039\/501100000736","name":"University of East Anglia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000736","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000774","name":"Newcastle University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000774","id-type":"DOI","asserted-by":"publisher"}]},{"name":"UK Health Security Agency"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The current, seventh cholera pandemic (7PET) is notably different from the previous six, owing to its rapid global spread and increased transmission of antimicrobial resistance. The global emergence of MDR Vibrio cholerae is a public health concern. We aimed to interrogate WGS data from V. cholerae isolates referred to the UK Health Security Agency (formerly Public Health England, PHE) for the presence of blaPER-7 encoding resistance to the extended-spectrum cephalosporins.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We reviewed 161 genomes of V. cholerae isolated between 2019 and 2024 from travellers returning to the UK, screening this dataset for resistance to the extended-spectrum cephalosporins encoded by blaPER-7.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We identified 3\/51 V. cholerae O1 ST69 isolates and 1\/110 non-O1 isolate belonging to ST555, harbouring blaPER-7 alongside mph(A), encoding azithromycin resistance. Long-read sequencing confirmed that both these genes were on a YemVchMDR1 element, that also possessed an MDR island carrying genes encoding resistance to aminoglycosides, sulphonamides and florfenicol. This element was located on an IncC plasmid in the ST69 O1 isolates but had inserted into the chromosome of the non-O1 isolate.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Public health institutions have the ability and responsibility to monitor the burden and spread of highly drug-resistant V. cholerae via passive surveillance, informing clinical guidance, empirical treatment and travel advice.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaf232","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T07:44:22Z","timestamp":1751355862000},"page":"2428-2432","source":"Crossref","is-referenced-by-count":7,"title":["Highly drug-resistant <i>Vibrio cholerae<\/i> harbouring <i>bla<\/i>PER-7 isolated from travellers returning to England"],"prefix":"10.1093","volume":"80","author":[{"given":"Satheesh","family":"Nair","sequence":"first","affiliation":[{"name":"Gastrointestinal Bacteria Reference Unit, UK Health Security Agency , London NW9 5HT ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare R","family":"Barker","sequence":"additional","affiliation":[{"name":"Gastrointestinal Bacteria Reference Unit, UK Health Security Agency , London NW9 5HT ,","place":["UK"]},{"name":"NIHR Health Protection Research Unit in Gastrointestinal Infections, University of East Anglia , Norwich ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vineet","family":"Patel","sequence":"additional","affiliation":[{"name":"Gastrointestinal Bacteria Reference Unit, UK Health Security Agency , London NW9 5HT ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Ying","family":"Poh","sequence":"additional","affiliation":[{"name":"Gastrointestinal Bacteria Reference Unit, UK Health Security Agency , London NW9 5HT ,","place":["UK"]},{"name":"NIHR Health Protection Research Unit in Gastrointestinal Infections, University of East Anglia , Norwich ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R","family":"Greig","sequence":"additional","affiliation":[{"name":"Gastrointestinal Bacteria Reference Unit, UK Health Security Agency , London NW9 5HT ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Israel","family":"Olonade","sequence":"additional","affiliation":[{"name":"Gastrointestinal Bacteria Reference Unit, UK Health Security Agency , London NW9 5HT ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5599-3933","authenticated-orcid":false,"given":"Paolo","family":"Ribeca","sequence":"additional","affiliation":[{"name":"Gastrointestinal Bacteria Reference Unit, UK Health Security Agency , London NW9 5HT ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Gastrointestinal Bacteria Reference Unit, UK Health Security Agency , London NW9 5HT ,","place":["UK"]},{"name":"NIHR Health Protection Research Unit in Gastrointestinal Infections, University of East Anglia , Norwich ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"2025090219520398400_dkaf232-B1","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/S0140-6736(03)15328-7","article-title":"Cholera","volume":"363","author":"Sack","year":"2004","journal-title":"Lancet"},{"key":"2025090219520398400_dkaf232-B2","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1038\/s41586-018-0818-3","article-title":"Genomic insights into the 2016\u20132017 cholera epidemic in Yemen","volume":"565","author":"Weill","year":"2019","journal-title":"Nature"},{"key":"2025090219520398400_dkaf232-B3","doi-asserted-by":"publisher","first-page":"1787","DOI":"10.1038\/s41564-023-01472-1","article-title":"Genomic epidemiology reveals multidrug resistant plasmid spread between Vibrio cholerae lineages in Yemen","volume":"8","author":"Lassalle","year":"2023","journal-title":"Nat Microbiol"},{"key":"2025090219520398400_dkaf232-B4","doi-asserted-by":"publisher","first-page":"6963","DOI":"10.1038\/s41467-024-51428-0","article-title":"An unusual two-strain cholera outbreak in Lebanon, 2022-2023: a genomic epidemiology study","volume":"15","author":"Abou Fayad","year":"2024","journal-title":"Nat Commun"},{"key":"2025090219520398400_dkaf232-B5","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1056\/NEJMc2004773","article-title":"Highly resistant cholera outbreak strain in Zimbabwe","volume":"383","author":"Mashe","year":"2020","journal-title":"N Engl J Med"},{"key":"2025090219520398400_dkaf232-B6","doi-asserted-by":"publisher","first-page":"144","DOI":"10.3389\/fmicb.2016.00144","article-title":"Extraintestinal infections caused by non-toxigenic Vibrio cholerae non-O1\/non-O139","volume":"7","author":"Chowdhury","year":"2016","journal-title":"Front Microbiol"},{"key":"2025090219520398400_dkaf232-B7","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1046\/j.1469-0691.2000.00174.x","article-title":"Clinical manifestations and characterization of extra-intestinal Vibrio cholerae non-O1, non-O139 infections in Denmark","volume":"6","author":"Dalsgaard","year":"2000","journal-title":"Clin Microbiol Infect"},{"key":"2025090219520398400_dkaf232-B8","doi-asserted-by":"publisher","first-page":"1951","DOI":"10.3201\/eid2011.140889","article-title":"Multidrug-resistant IncA\/C plasmid in Vibrio cholerae from Haiti","volume":"20","author":"Folster","year":"2014","journal-title":"Emerg Infect Dis"},{"key":"2025090219520398400_dkaf232-B9","doi-asserted-by":"publisher","first-page":"2400069","DOI":"10.2807\/1560-7917.ES.2024.29.36.2400069","article-title":"Genomic analysis of Vibrio cholerae O1 isolates from cholera cases, Europe, 2022","volume":"29","author":"Rouard","year":"2024","journal-title":"Euro Surveill"},{"key":"2025090219520398400_dkaf232-B10","doi-asserted-by":"publisher","first-page":"2271","DOI":"10.1056\/NEJMc2408761","article-title":"Long-distance spread of a highly drug-resistant epidemic cholera strain","volume":"391","author":"Rouard","year":"2024","journal-title":"N Engl J Med"},{"key":"2025090219520398400_dkaf232-B11","doi-asserted-by":"publisher","first-page":"e00831-18","DOI":"10.1128\/JCM.00831-18","article-title":"Evaluation of whole-genome sequencing for identification and typing of Vibrio cholerae","volume":"56","author":"Greig","year":"2018","journal-title":"J Clin Microbiol"},{"key":"2025090219520398400_dkaf232-B12","doi-asserted-by":"publisher","first-page":"2300","DOI":"10.1093\/jac\/dkw093","article-title":"Detection of the plasmid-mediated mcr-1 gene conferring colistin resistance in human and food isolates of Salmonella enterica and Escherichia coli in England and Wales","volume":"71","author":"Doumith","year":"2016","journal-title":"J Antimicrob Chemother"},{"key":"2025090219520398400_dkaf232-B13","doi-asserted-by":"publisher","first-page":"mgen000545","DOI":"10.1099\/mgen.0.000545","article-title":"Analysis of a small outbreak of Shiga toxin-producing Escherichia coli O157:H7 using long-read sequencing","volume":"7","author":"Greig","year":"2021","journal-title":"Microb Genom"},{"key":"2025090219520398400_dkaf232-B14","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1186\/s13059-014-0524-x","article-title":"The Harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes","volume":"15","author":"Treangen","year":"2014","journal-title":"Genome Biol"},{"key":"2025090219520398400_dkaf232-B15","doi-asserted-by":"publisher","first-page":"e000093","DOI":"10.1099\/mgen.0.000093","article-title":"Microreact: visualizing and sharing data for genomic epidemiology and phylogeography","volume":"2","author":"Argim\u00f3n","year":"2016","journal-title":"Microb Genom"},{"key":"2025090219520398400_dkaf232-B16","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1093\/bioinformatics\/btr039","article-title":"Easyfig: a genome comparison visualizer","volume":"27","author":"Sullivan","year":"2011","journal-title":"Bioinformatics"},{"key":"2025090219520398400_dkaf232-B17","doi-asserted-by":"publisher","first-page":"1199","DOI":"10.1016\/S0140-6736(24)01867-1","article-title":"Global burden of bacterial antimicrobial resistance 1990\u20132021: a systematic analysis with forecasts to 2050","volume":"404","author":"GBD 2021 Antimicrobial Resistance Collaborators","year":"2024","journal-title":"Lancet"},{"key":"2025090219520398400_dkaf232-B18","doi-asserted-by":"publisher","first-page":"e02408-18","DOI":"10.1128\/mBio.02408-18","article-title":"Acquisition and loss of CTX-M-producing and non-producing Escherichia coli in the fecal microbiome of travelers to South Asia","volume":"9","author":"Bevan","year":"2018","journal-title":"mBio"},{"key":"2025090219520398400_dkaf232-B19","doi-asserted-by":"publisher","first-page":"796","DOI":"10.3390\/antibiotics9110796","article-title":"High prevalence of blaCTX-M-15 gene among extended-spectrum \u03b2-lactamase-producing Escherichia coli isolates causing extraintestinal infections in Bangladesh","volume":"9","author":"Mazumder","year":"2020","journal-title":"Antibiotics (Basel)"},{"key":"2025090219520398400_dkaf232-B20","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.jhin.2021.03.030","article-title":"Molecular characterization of plasmids encoding blaCTX-M from faecal Escherichia coli in travellers returning to the UK from South Asia","volume":"114","author":"Bevan","year":"2021","journal-title":"J Hosp Infect"},{"key":"2025090219520398400_dkaf232-B21","doi-asserted-by":"publisher","first-page":"862234","DOI":"10.3389\/fmicb.2022.862234","article-title":"Use of nanopore sequencing to characterise the genomic architecture of mobile genetic elements encoding blaCTX-M-15 in Escherichia coli causing travellers\u2019 diarrhoea","volume":"13","author":"Bird","year":"2022","journal-title":"Front Microbiol"},{"key":"2025090219520398400_dkaf232-B22","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1016\/j.ijid.2021.02.102","article-title":"Clinical and socio-environmental determinants of multidrug-resistant Vibrio cholerae O1 in older children and adults in Bangladesh","volume":"105","author":"Garbern","year":"2021","journal-title":"Int J Infect Dis"},{"key":"2025090219520398400_dkaf232-B23","doi-asserted-by":"publisher","first-page":"652","DOI":"10.4269\/ajtmh.20-0058","article-title":"Changing susceptibility pattern of Vibrio cholerae O1 isolates to commonly used antibiotics in the largest diarrheal disease hospital in Bangladesh during 2000\u20132018","volume":"103","author":"Parvin","year":"2020","journal-title":"Am J Trop Med Hyg"},{"key":"2025090219520398400_dkaf232-B24","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1093\/jac\/dkad391","article-title":"Vibrio species: development of EUCAST susceptibility testing methods and MIC and zone diameter distributions on which to determine clinical breakpoints","volume":"79","author":"Karatuna","year":"2024","journal-title":"J Antimicrob Chemother"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/80\/9\/2428\/63780534\/dkaf232.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/80\/9\/2428\/63780534\/dkaf232.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T23:52:07Z","timestamp":1756857127000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/80\/9\/2428\/8203696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,17]]},"references-count":24,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,7,17]]},"published-print":{"date-parts":[[2025,9,3]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkaf232","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,9]]},"published":{"date-parts":[[2025,7,17]]}}}