{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:49:11Z","timestamp":1792622951360,"version":"4.5.0"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100014882","name":"Hebei Provincial Department of Finance","doi-asserted-by":"publisher","award":["ZF2025116"],"award-info":[{"award-number":["ZF2025116"]}],"id":[{"id":"10.13039\/501100014882","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To establish patterns of the antimicrobial susceptibility of Nocardia species to carbapenems and fluoroquinolones and analysis of phenotypic\u2013genotypic correlations.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Isolates were identified to the species using 16S rRNA, secA1, or rpoB gene sequencing analysis. The antimicrobial susceptibility testing was performed using the broth microdilution method, and WGS was employed to analyse the presence of resistance genes and\/or mutations of Nocardia species against carbapenems and fluoroquinolones.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Among 143 Nocardia isolates, N. farcinica (27.27%, 39\/143) and N. cyriacigeorgica (25.17%, 36\/143) were the most common species, followed by N. abscessus Complex (18.88%, 27\/143). The MIC90s of the seven carbapenems were 8\u2005mg\/L for doripenem, 8\u2005mg\/L for meropenem, 16\u2005mg\/L for ertapenem, 16\u2005mg\/L for biapenem, 64\u2005mg\/L for imipenem, 64\u2005mg\/L for faropenem and 128\u2005mg\/L for tebipenem, respectively. The susceptibility rates to imipenem were 76.9% and 88.9% for N. farcinica and N. cyriacigeorgica, respectively, but only 14.3% and 0% for N. otitidiscavarium and N. brasiliensis, respectively. Further, 90% of N. brasiliensis and 50% of N. otitidiscaviarum isolates were susceptible and intermediate to meropenem. WGS identified blaFAR-1 gene in N. farcinica and blaAST-1 gene in N. cyriacigeorgica, respectively. The MIC90s of the four fluoroquinolones were 1\u2005mg\/L for sitafloxacin, 4\u2005mg\/L for nemonoxacin, 4\u2005mg\/L for moxifloxacin and 16\u2005mg\/L for ciprofloxacin, respectively. The susceptibility rate of Nocardia species to ciprofloxacin was low except for N. farcinica. The resistance to fluoroquinolones arise from mutations in the gyrA gene.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Nocardia spp. exhibited varying patterns of susceptibility to carbapenems and fluoroquinolones respectively. Importantly, different Nocardia spp. exhibited different patterns of susceptibility to carbapenems and fluoroquinolones, respectively.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaf312","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T14:22:49Z","timestamp":1756390969000},"page":"2862-2872","source":"Crossref","is-referenced-by-count":7,"title":["Phenotypic and genotypic profiles of clinical isolates of various <i>Nocardia<\/i> species to carbapenems and fluoroquinolones"],"prefix":"10.1093","volume":"80","author":[{"given":"Jing","family":"Yang","sequence":"first","affiliation":[{"name":"Hebei Provincial Center for Clinical Laboratories, The Second Hospital of Hebei Medical University , Shijiazhuang 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