{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T12:21:03Z","timestamp":1776687663308,"version":"3.51.2"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"National High Level Hospital Clinical Research","award":["2022-PUMCH-B-074"],"award-info":[{"award-number":["2022-PUMCH-B-074"]}]},{"name":"Special Foundation for National Science and Technology Basic Research Program","award":["2019FY101200"],"award-info":[{"award-number":["2019FY101200"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the in vitro activity of contezolid against clinical Staphylococcus and Enterococcus isolates from across China, and compare its performance with linezolid and other key agents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 2510 non-duplicate Gram-positive cocci isolates were collected from 70 hospitals in seven Chinese regions. Minimum inhibitory concentrations (MICs) were determined using broth microdilution (BMD) per CLSI guidelines. MIC50\/90 values, susceptibility rates, cumulative MIC curves and MIC distribution agreement between contezolid and linezolid were assessed. Linezolid-resistant isolates and contezolid-resistant isolates\u2014defined as those based on CLSI 2025 linezolid breakpoints\u2014underwent whole-genome sequencing and comprehensive screening for known oxazolidinone resistance determinants, including acquired resistance genes, 23S rRNA mutations and amino acid substitutions in ribosomal proteins L3, L4 and L22.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Contezolid exhibited potent in vitro activity, with &amp;gt;99% of isolates inhibited at \u22644\u202fmg\/L and showed lower MIC50\/90 than linezolid, with a consistently left-shifted cumulative MIC distribution. Cohen\u2019s kappa analysis supporting enhanced activity of contezolid. Notably, we report for the first time clinical isolates with contezolid MICs up to 16\u202fmg\/L. Whole-genome analysis of 14 linezolid-resistant isolates (including five resistant to contezolid) revealed universal presence of optrA, cfr, fexA and other resistance genes, alongside domain V 23S rRNA mutations and substitutions in ribosomal proteins L3 and L4, supporting a shared genetic basis and potential cross-resistance between the two agents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Contezolid demonstrated potent and consistent activity against drug-resistant Gram-positive cocci, supporting its potential as a therapeutic alternative to linezolid. Further studies are needed to confirm resistance mechanisms and clinical utility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkaf331","type":"journal-article","created":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T22:22:05Z","timestamp":1762640525000},"page":"3011-3020","source":"Crossref","is-referenced-by-count":1,"title":["<i>In vitro<\/i>\n                    activity of contezolid and other comparators against clinical\n                    <i>Staphylococcus<\/i>\n                    and\n                    <i>Enterococcus<\/i>\n                    : a multicenter study in China"],"prefix":"10.1093","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7978-0967","authenticated-orcid":false,"given":"Rongqi","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 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