{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T22:34:58Z","timestamp":1762641298487,"version":"build-2065373602"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province of China","doi-asserted-by":"publisher","award":["LH2024C061"],"award-info":[{"award-number":["LH2024C061"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["1610302024001"],"award-info":[{"award-number":["1610302024001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To identify and characterize the oxazolidinone resistance genes cfr and optrA from a methicillin-resistant Macrococcoides bohemicum strain of chicken origin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The presence of mobile oxazolidinone resistance genes was detected by PCR. Antimicrobial susceptibility testing was conducted by broth microdilution. Transfer experiments were carried out to evaluate horizontal transferability of the plasmid. WGS was performed using a combination of Illumina NovaSeq\/Oxford Nanopore PromethION platforms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The M. bohemicum strain HLJ23 exhibited an MDR phenotype and was positive for both cfr and optrA genes. WGS revealed that the genes cfr and optrA co-exist on the novel MDR plasmid pHLJ23-71kb. Although conjugation experiments were unsuccessful, plasmid pHLJ23-71kb could be transferred to Staphylococcus aureus RN4220 by electrotransformation. Genetic context analysis showed that the cfr and optrA together with another four antimicrobial resistance genes are located in an MDR region on plasmid pHLJ23-71kb. Sequence analysis suggested that this MDR region possibly originated from Mammaliicoccus or Staphylococcus spp.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>To the best of our knowledge, this study represents the first report of the oxazolidinone resistance genes cfr and optrA in the genus Macrococcoides. Furthermore, attention should be paid to the exchange of resistance determinants between members of the genera Staphylococcus, Mammaliicoccus and Macrococcoides.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkaf333","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T11:44:05Z","timestamp":1756381445000},"page":"3021-3025","source":"Crossref","is-referenced-by-count":0,"title":["Co-existence of the oxazolidinone resistance genes\n                    <i>cfr<\/i>\n                    and\n                    <i>optrA<\/i>\n                    on a novel multiresistance plasmid from a methicillin-resistant\n                    <i>Macrococcoides bohemicum<\/i>\n                    strain"],"prefix":"10.1093","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7390-6872","authenticated-orcid":false,"given":"Yao","family":"Zhu","sequence":"first","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susu","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6303-8212","authenticated-orcid":false,"given":"Stefan","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin 14163 ,","place":["Germany"]},{"name":"Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin 14163 ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Hou","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiu","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Longhua","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiyun","family":"Chai","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caiping","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongfei","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangyu","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongshan","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9171-8637","authenticated-orcid":false,"given":"Wanjiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,9,22]]},"reference":[{"key":"2025110817252432200_dkaf333-B1","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.ijantimicag.2003.11.003","article-title":"Oxazolidinones: activity, mode of action, and mechanism of resistance","volume":"23","author":"Bozdogan","year":"2004","journal-title":"Int J Antimicrob Agents"},{"key":"2025110817252432200_dkaf333-B2","doi-asserted-by":"publisher","first-page":"e0018820","DOI":"10.1128\/CMR.00188-20","article-title":"Mobile oxazolidinone resistance genes in gram-positive and gram-negative bacteria","volume":"34","author":"Schwarz","year":"2021","journal-title":"Clin Microbiol Rev"},{"key":"2025110817252432200_dkaf333-B3","doi-asserted-by":"publisher","first-page":"2596","DOI":"10.1093\/jac\/dkac263","article-title":"Oxazolidinones: mechanisms of resistance and mobile genetic elements involved","volume":"77","author":"Brenciani","year":"2022","journal-title":"J Antimicrob Chemother"},{"key":"2025110817252432200_dkaf333-B4","doi-asserted-by":"publisher","first-page":"2500","DOI":"10.1128\/AAC.00131-06","article-title":"The Cfr rRNA methyltransferase confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics","volume":"50","author":"Long","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"2025110817252432200_dkaf333-B5","doi-asserted-by":"publisher","first-page":"2182","DOI":"10.1093\/jac\/dkv116","article-title":"A novel gene, optrA, that confers transferable resistance to oxazolidinones and phenicols and its presence in Enterococcus faecalis and Enterococcus faecium of human and animal origin","volume":"70","author":"Wang","year":"2015","journal-title":"J Antimicrob Chemother"},{"key":"2025110817252432200_dkaf333-B6","doi-asserted-by":"publisher","first-page":"e01975","DOI":"10.1128\/mBio.01975-15","article-title":"ABC-F proteins mediate antibiotic resistance through ribosomal protection","volume":"7","author":"Sharkey","year":"2016","journal-title":"mBio"},{"key":"2025110817252432200_dkaf333-B7","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1093\/jac\/dkab478","article-title":"Characterization of the novel optrA-carrying pseudo-compound transposon Tn7363 and an Inc18 plasmid carrying cfr(D) in Vagococcus lutrae","volume":"77","author":"Zhu","year":"2022","journal-title":"J Antimicrob Chemother"},{"key":"2025110817252432200_dkaf333-B8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/bs.aambs.2018.05.002","article-title":"The genus Macrococcus: an insight into its biology, evolution, and relationship with Staphylococcus","volume":"105","author":"Mazhar","year":"2018","journal-title":"Adv Appl Microbiol"},{"key":"2025110817252432200_dkaf333-B9","doi-asserted-by":"publisher","first-page":"1181376","DOI":"10.3389\/fmicb.2023.1181376","article-title":"Genus-wide genomic characterization of Macrococcus: insights into evolution, population structure, and functional potential","volume":"14","author":"Carroll","year":"2023","journal-title":"Front Microbiol"},{"key":"2025110817252432200_dkaf333-B10","doi-asserted-by":"publisher","first-page":"1796","DOI":"10.1093\/jac\/dkac107","article-title":"The bla and mec families of beta-lactam resistance genes in the genera Macrococcus, Mammaliicoccus and Staphylococcus: an in-depth analysis with emphasis on Macrococcus","volume":"77","author":"Schwendener","year":"2022","journal-title":"J Antimicrob Chemother"},{"key":"2025110817252432200_dkaf333-B11","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1007\/s10482-023-01857-6","article-title":"Phylogenomic and molecular markers based studies on Staphylococcaceae and Gemella species. Proposals for an emended family Staphylococcaceae and three new families (Abyssicoccaceae fam. nov., Salinicoccaceae fam. nov. and Gemellaceae fam. nov.) harboring four new genera, Lacicoccus gen. nov., Macrococcoides gen. nov., Gemelliphila gen. nov., and Phocicoccus gen. nov","volume":"116","author":"Bello","year":"2023","journal-title":"Antonie Van Leeuwenhoek"},{"author":"CLSI","key":"2025110817252432200_dkaf333-B12"},{"key":"2025110817252432200_dkaf333-B13","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1093\/jac\/dkab456","article-title":"Occurrence of a plasmid co-carrying cfr(D) and poxtA2 linezolid resistance genes in Enterococcus faecalis and Enterococcus casseliflavus from porcine manure, Italy","volume":"77","author":"Cinthi","year":"2022","journal-title":"J Antimicrob Chemother"},{"key":"2025110817252432200_dkaf333-B14","doi-asserted-by":"publisher","first-page":"590","DOI":"10.3390\/antibiotics9090590","article-title":"Methicillin-resistant Macrococcus bohemicus encoding a divergent SCCmecB element","volume":"9","author":"Foster","year":"2020","journal-title":"Antibiotics (Basel)"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/80\/11\/3021\/64344792\/dkaf333.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/80\/11\/3021\/64344792\/dkaf333.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T22:25:28Z","timestamp":1762640728000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/80\/11\/3021\/8261408"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,22]]},"references-count":14,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,9,22]]},"published-print":{"date-parts":[[2025,11,4]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkaf333","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"type":"print","value":"0305-7453"},{"type":"electronic","value":"1460-2091"}],"subject":[],"published-other":{"date-parts":[[2025,11]]},"published":{"date-parts":[[2025,9,22]]}}}