{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T22:34:53Z","timestamp":1762641293471,"version":"build-2065373602"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T00:00:00Z","timestamp":1759708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003323","name":"Agence Nationale de Recherches sur le Sida et les hepatites virales","doi-asserted-by":"publisher","award":["ANRS12406-EvvA"],"award-info":[{"award-number":["ANRS12406-EvvA"]}],"id":[{"id":"10.13039\/501100003323","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012947","name":"IRD","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012947","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We previously reported a high prevalence of HIV drug resistance mutations (DRMs) among adolescents with a viral load (VL)\u200a\u2265\u200a200 copies\/mL. In this study, we complement this analysis by simultaneously characterizing circulating and archived viruses in order to obtain a more comprehensive drug resistance profile.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using 127 plasma and paired buffy-coat samples collected between February and September 2021 from 90 adolescents (10\u201319 years) with a VL\u200a\u2265\u200a200 copies\/mL, we performed genotyping resistance testing on both circulating and archived viral strains using an in-house Sanger method targeting the reverse transcriptase, protease and integrase genes. The Stanford HIValg programme was used to identify relevant DRMs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We generated 97 (76.4%) paired archived and circulating viral sequences, of which 60 (61.9%) were recombinant CRF02_AG. Globally, about 8\/10 participants harboured DRMs to at least one drug class in circulant (80.4%) and archived (77.3%) viruses, slightly higher in samples with VL between 200 and 999 copies\/mL and mainly NRTIs and NNRTI-associated resistance. One-third of the participants had cross-resistance to the newer NNRTIs rilpivirine and doravirine, which are not yet available in our settings. More than half (56.7%) of the DRMs were detected in both viral populations, but 2-fold more DRMs were found exclusively in circulant strains (29.8% versus 13.4% in archived viruses).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The high level of resistance found in this study, coupled with the identification of DRMs either in circulating and archived viruses, only suggests that a comprehensive profiling of DRMs in both viral populations would provide additional information for adolescents with a detectable viremia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkaf356","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T11:32:18Z","timestamp":1757417538000},"page":"3160-3164","source":"Crossref","is-referenced-by-count":0,"title":["High level of archived and circulating HIV-1 drug resistance mutations in adolescents with a detectable viremia in Cameroon"],"prefix":"10.1093","volume":"80","author":[{"given":"Armando B D","family":"Djiyou","sequence":"first","affiliation":[{"name":"Pathogens, Epidemiology and Clinical Trials Laboratory, Postgraduate Training Unit for Health Sciences, Postgraduate School for Pure and Applied Sciences, The University of Douala , PO Box 2701, Douala ,","place":["Cameroon"]},{"name":"MIVEGEC, Universit\u00e9 de Montpellier, CNRS, IRD , Montpellier ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1070-2108","authenticated-orcid":false,"given":"Calixte I","family":"Penda","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala , Douala ,","place":["Cameroon"]},{"name":"Department of Pediatrics and Child Health, General Hospital of Douala , Douala 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