{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T09:17:33Z","timestamp":1778923053689,"version":"3.51.4"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T00:00:00Z","timestamp":1778889600000},"content-version":"vor","delay-in-days":11,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["PLEC2021-007972"],"award-info":[{"award-number":["PLEC2021-007972"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministry of Science, Innovation and Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["PLEC2024-011123"],"award-info":[{"award-number":["PLEC2024-011123"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Carbapenemase-producing Gram-negative bacteria represent a major clinical challenge. Rapid detection and characterization of carbapenemase group is essential for guiding therapy and for infection control. We evaluated the performance of Certest ResisCheck\u00ae Carbapenemases, a novel lateral flow immunoassay (LFIA), for detecting the main carbapenemase groups from bacterial culture.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>A multicentre collection of 351 well-characterized clinical Gram-negative isolates, studied by whole-genome sequencing, was tested using this assay. 57 KPC, 53 NDM, 63 VIM, 68 OXA-48-like, 64 IMP and 100 carbapenem resistant non-carbapenemase producers were included.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The LFIA showed an overall sensitivity of 96.4% and specificity of 100%. The positive predictive value (PPV) was 100%, and the negative predictive value (NPV) was 91.7%. No false-positives or cross-reactions with extended-spectrum \u03b2-lactamases (ESBLs) or cephalosporinases were observed either with isolates harbouring multiple carbapenemases. Detection rates for IMP and NDM variants reached 100%. Sensitivity for KPC, OXA-like and VIM was slightly lower (93.0%, 95.6% and 96.8%, respectively). Nine false-negative results were observed: KPC-31 (n\u200a=\u200a4), VIM-63 (n\u200a=\u200a2) and OXA-1054-producing (n\u200a=\u200a3) isolates. Increasing the bacterial inoculum managed to obtain a positive result for KPC-31 and VIM-63, but OXA-1054 remained undetected.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Certest ResisCheck\u00ae Carbapenemases is a rapid, accurate, and reliable tool for detecting carbapenemases in clinical settings. While some rare variants may escape detection, its strong performance across diverse bacterial species supports its use as part of routine diagnostics to guide timely antimicrobial therapy and control the spread of resistance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkag149","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T11:29:50Z","timestamp":1777980590000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of a novel lateral flow immunochromatographic assay, Certest ResisCheck\u00ae Carbapenemases, for the rapid detection and differentiation of carbapenemase in Gram-negative bacteria from bacterial culture"],"prefix":"10.1093","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3476-9737","authenticated-orcid":false,"given":"Laura","family":"Monge-Olivares","sequence":"first","affiliation":[{"name":"Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena\/CSIC\/Universidad de Sevilla , Seville, SE 41009 ,","place":["Spain"]},{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad de Sevilla , Seville, SE 41009 ,","place":["Spain"]},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III , Madrid, MA 28220 ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0204-1303","authenticated-orcid":false,"given":"Inmaculada","family":"L\u00f3pez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena\/CSIC\/Universidad de Sevilla , Seville, SE 41009 ,","place":["Spain"]},{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad de Sevilla , Seville, SE 41009 ,","place":["Spain"]},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III , Madrid, MA 28220 ,","place":["Spain"]},{"name":"Unidad de Microbiolog\u00eda y Enfermedades Infecciosas, Hospital Universitario Virgen, Macarena , Seville, SE 41009 ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6597-6130","authenticated-orcid":false,"given":"Felipe","family":"Fern\u00e1ndez-Cuenca","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena\/CSIC\/Universidad de Sevilla , Seville, SE 41009 ,","place":["Spain"]},{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad de Sevilla , Seville, SE 41009 ,","place":["Spain"]},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III , Madrid, MA 28220 ,","place":["Spain"]},{"name":"Unidad de Microbiolog\u00eda y Enfermedades Infecciosas, Hospital Universitario Virgen, Macarena , Seville, SE 41009 ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8672-5891","authenticated-orcid":false,"given":"\u00c1lvaro","family":"Pascual Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena\/CSIC\/Universidad de Sevilla , Seville, SE 41009 ,","place":["Spain"]},{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad de Sevilla , Seville, SE 41009 ,","place":["Spain"]},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III , Madrid, MA 28220 ,","place":["Spain"]},{"name":"Unidad de Microbiolog\u00eda y Enfermedades Infecciosas, Hospital Universitario Virgen, Macarena , Seville, SE 41009 ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8950-4384","authenticated-orcid":false,"given":"Lorena","family":"L\u00f3pez-Cerero","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena\/CSIC\/Universidad de Sevilla , Seville, SE 41009 ,","place":["Spain"]},{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad de Sevilla , Seville, SE 41009 ,","place":["Spain"]},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III , Madrid, MA 28220 ,","place":["Spain"]},{"name":"Unidad de Microbiolog\u00eda y Enfermedades Infecciosas, Hospital Universitario Virgen, Macarena , Seville, SE 41009 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