{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T10:02:38Z","timestamp":1785319358274,"version":"3.55.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1800600"],"award-info":[{"award-number":["2021YFD1800600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and objectives<\/jats:title>\n                    <jats:p>Mycoplasma pneumoniae is the primary pathogen of community-acquired bacterial pneumonia in humans. Children and adolescents are particularly susceptible, and a dry cough is the most common clinical symptom. In severe cases, this may progress to acute respiratory distress syndrome. To determine the anti-M. pneumoniae and anti-inflammatory activities of a novel pleuromutilin derivative, 22-(4-((2-furan-1-yl)acetamido)phenyl)thio) deoxypleuromutilin (p-furoylamphenmulin, or PFAPM).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We utilized M. pneumoniae-infected mice to conduct in vivo pharmacokinetic (PK) and pharmacodynamic (PD) studies. The recommended dose was calculated based on PK\/PD analysis, and treatment trials were performed at this dose to validate the fitted outcomes and investigate the anti-inflammatory effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The PK\/PD results indicated that PFAPM exhibited an absorption half-life (T1\/2ka) of 0.07\u2005h and an elimination half-life (T1\/2kel) of 0.87\u2005h, with corresponding AUC values ranging from 1.18 to 8.33 \u03bcg\u00b7h\/mL at doses of 10\u201380\u2005mg\/kg. When the mycoplasma load decreased by 3 log10 cfu\/mL, the corresponding AUC48h\/MIC and Cmax\/MIC values were 7617.62\u2005h and 1256.76, respectively, at a dose of 67.66\u2005mg\/kg. ELISA and flow cytometry analysis revealed that drug treatment significantly reduced cytokines and inflammatory cells associated with T helper 1 (Th1) and T helper 2 (Th2) immune responses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>AUC48h\/MIC was the best parameter to describe the PK\/PD relationship. The bactericidal effect was achieved at a dose of 67.66 (\u223c68) mg\/kg. Pleuromutilins can reduce Th1 and Th2 inflammation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkag225","type":"journal-article","created":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T11:37:45Z","timestamp":1784979465000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>In vivo<\/i>\n                    PK\/PD integration of\n                    <i>p<\/i>\n                    -furoylamphenmulin against\n                    <i>Mycoplasma pneumoniae<\/i>\n                    and its anti-inflammatory efficacy"],"prefix":"10.1093","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2039-5583","authenticated-orcid":false,"given":"Xirui","family":"Xia","sequence":"first","affiliation":[{"name":"Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University , 483 Wushan Road, Tianhe District, Guangzhou, Guangdong Province 510642 ,","place":["China"]},{"name":"Frontier Technology Research Institute of China Agricultural University in Shenzhen, China Agricultural University , 140 Jinye Avenue, Sanxi Community, Kuichong Subdistrict, Dapeng New District, Shenzhen, Guangdong Province 518116 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University , 483 Wushan Road, Tianhe District, Guangzhou, Guangdong Province 510642 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University , 483 Wushan Road, Tianhe District, Guangzhou, 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