{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T10:15:58Z","timestamp":1783419358925,"version":"3.54.6"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"\u2018Sant\u00e9 Publique France\u2019"},{"name":"French national public health agency"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To assess the epidemiological susceptibility distribution of five \u03b2-lactam antibiotics for contemporary clinical isolates of non-faecalis Enterococcus species categorized as susceptible to ampicillin and to evaluate the performance of gradient diffusion (GD) tests compared to broth microdilution (BMD), the reference method for antimicrobial susceptibility testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 455 non-duplicate non-Enterococcus faecalis clinical isolates reported as ampicillin susceptible were collected from 43 French hospital laboratories. Identification to the species level was performed using MALDI-TOF mass spectrometry. MICs of amoxicillin, piperacillin, imipenem, meropenem and ceftobiprole were determined by BMD and GD. Results were interpreted according to the 2025 EUCAST recommendations. Agreement between GD and BMD was assessed using ISO and FDA criteria.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All isolates were susceptible to amoxicillin, 94% (428\/455) to ceftobiprole, 88% (401\/455) to imipenem at high dose and 88% (402\/455) to piperacillin. Enterococcus casseliflavus displayed the lowest MIC values across all \u03b2-lactams, followed by Enterococcus avium. In contrast, Enterococcus faecium, Enterococcus lactis, Enterococcus gallinarum and Enterococcus hirae exhibited right-shifted MIC distributions. Most species showed high MICs for meropenem, indicating low intrinsic activity. GD methods did not achieve FDA or ISO criteria for most species-antibiotic combinations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Overall, ampicillin may predict amoxicillin categorization for frequent non-faecalis enterococci, and with the exception of meropenem, most \u03b2-lactams may still have clinical value against these species. The different species exhibit distinct MIC distribution patterns for the tested \u03b2-lactams. Furthermore, substantial discrepancies observed between GD and BMD methods highlight the limited reliability of GD as an alternative to BMD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkag227","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T09:41:55Z","timestamp":1783417315000},"source":"Crossref","is-referenced-by-count":0,"title":["\u03b2-lactam susceptibility testing of ampicillin-susceptible non-\n                    <i>faecalis<\/i>\n                    enterococcal clinical isolates in France"],"prefix":"10.1093","volume":"81","author":[{"given":"Marl\u00e8ne","family":"Amara","sequence":"first","affiliation":[{"name":"CH de Versailles, Service de Biologie, Unit\u00e9 de microbiologie , Le Chesnay 78157 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Coiffard","sequence":"additional","affiliation":[{"name":"CH de Versailles, Service de Biologie, Unit\u00e9 de microbiologie , Le Chesnay 78157 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6821-3131","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Gu\u00e9rin","sequence":"additional","affiliation":[{"name":"CHU de Rennes, Service de Bact\u00e9riologie-Hygi\u00e8ne Hospitali\u00e8re , Rennes ,","place":["France"]},{"name":"CNR de la R\u00e9sistance aux Antibiotiques (laboratoire associ\u00e9 \u2018Ent\u00e9rocoques\u2019) , CHU de Rennes, Rennes ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Godmer","sequence":"additional","affiliation":[{"name":"INSERM, U1135, Sorbonne Universit\u00e9, Centre d\u2019Immunologie et des Maladies Infectieuses, Cimi-Paris , Paris ,","place":["France"]},{"name":"D\u00e9partement de Bact\u00e9riologie, AP-HP, Sorbonne Universit\u00e9, H\u00f4pital Saint-Antoine , Paris ,","place":["France"]},{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UAR2024, Spectrom\u00e9trie de Masse pour la Biologie (MSBio), Plateforme Prot\u00e9omique , Paris ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6778-7504","authenticated-orcid":false,"given":"Gabriel","family":"Auger","sequence":"additional","affiliation":[{"name":"CHU de Rennes, Service de Bact\u00e9riologie-Hygi\u00e8ne Hospitali\u00e8re , Rennes ,","place":["France"]},{"name":"CNR de la R\u00e9sistance aux Antibiotiques (laboratoire associ\u00e9 \u2018Ent\u00e9rocoques\u2019) , CHU de Rennes, Rennes ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4026-9912","authenticated-orcid":false,"given":"Vincent","family":"Cattoir","sequence":"additional","affiliation":[{"name":"CHU de Rennes, Service de Bact\u00e9riologie-Hygi\u00e8ne Hospitali\u00e8re , Rennes ,","place":["France"]},{"name":"CNR de la R\u00e9sistance aux Antibiotiques (laboratoire associ\u00e9 \u2018Ent\u00e9rocoques\u2019) , CHU de Rennes, Rennes ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Col. BVH study group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mait\u00e9","family":"Micaelo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliosha","family":"Feuss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ornella","family":"Richide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00e9mie","family":"Blumenthal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thalie","family":"Viole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayane","family":"HANED","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Lemenand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Gallou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Brieu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Moquet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Henry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Plantin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Garnier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Baptiste","family":"Vuillemenot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Van Agt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Wilhelm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Sarah","family":"Fangous","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Le Gall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Courtellemont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Guinard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Laurent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Hombrouck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manica","family":"Vasseur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Poussing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Cady","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarisse","family":"Dupin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00e9mie","family":"Violette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Tournus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emeline","family":"Riverain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Vach\u00e9e","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Thouvenin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aymeric","family":"Coutard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Touroult-Jupin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Roudi\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Gregorowicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"GMC study group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9","family":"Plouzeau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Millot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Corvec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise Ruffier","family":"D'epenoux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9linda","family":"Cheminet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Ga\u00eblle","family":"Ranc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Laurent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul-Louis","family":"Woerther","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Teboul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Gallois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danna","family":"Assakaf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alban","family":"Le Monnier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assaf","family":"Mizrahi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Barraud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole","family":"Grelaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Le Brun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Bille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9ric","family":"Farfour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentine","family":"Latapy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Dortet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"2026070705415051500_dkag227-B1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00121-23","article-title":"Non-faecium non-faecalis enterococci: a review of clinical manifestations, virulence factors, and antimicrobial resistance","volume":"37","author":"Mullally","year":"2024","journal-title":"Clin Microbiol Rev"},{"key":"2026070705415051500_dkag227-B2","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.mib.2021.10.013","article-title":"The multifaceted lifestyle of enterococci: genetic diversity, ecology and risks for public health","volume":"65","author":"Cattoir","year":"2022","journal-title":"Curr Opin Microbiol"},{"key":"2026070705415051500_dkag227-B3","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/S0399-077X(05)80301-4","article-title":"R\u00e9sistance des ent\u00e9rocoques aux b\u00eata-lactamines et cons\u00e9quences sur les synergies","volume":"24","author":"Gutmann","year":"1994","journal-title":"Med Mal Infect"},{"key":"2026070705415051500_dkag227-B4","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.jiac.2018.01.001","article-title":"Clinical management of non-faecium non-faecalis vancomycin-resistant enterococci infection. Focus on Enterococcus gallinarum and Enterococcus casseliflavus\/flavescens","volume":"24","author":"Monticelli","year":"2018","journal-title":"J Infect Chemother"},{"key":"2026070705415051500_dkag227-B5","author":"EUCAST"},{"key":"2026070705415051500_dkag227-B6","author":"EUCAST"},{"key":"2026070705415051500_dkag227-B7","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01467-18","article-title":"Enterococcus faecium and ampicillin susceptibility determination: overestimation of resistance with disk diffusion method using 2 micrograms of ampicillin?","volume":"57","author":"Joste","year":"2019","journal-title":"J Clin Microbiol"},{"key":"2026070705415051500_dkag227-B8","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04545-22","article-title":"Ampicillin-susceptible Enterococcus faecium infections: clinical features, causal clades, and contribution of MALDI-TOF to early detection","volume":"11","author":"Faury","year":"2023","journal-title":"Microbiol Spectr"},{"key":"2026070705415051500_dkag227-B9","author":"EUCAST"},{"key":"2026070705415051500_dkag227-B10","author":"Center for Devices and Radiological Health"},{"key":"2026070705415051500_dkag227-B11","author":"ISO 20776-2:2021"},{"key":"2026070705415051500_dkag227-B12","author":"EUCAST"},{"key":"2026070705415051500_dkag227-B13","doi-asserted-by":"publisher","first-page":"170","DOI":"10.3390\/antibiotics10020170","article-title":"Ceftobiprole perspective: current and potential future indications","volume":"10","author":"Lupia","year":"2021","journal-title":"Antibiotics (Basel)"},{"key":"2026070705415051500_dkag227-B14","doi-asserted-by":"publisher","first-page":"3336","DOI":"10.1128\/jcm.31.12.3336-3339.1993","article-title":"Reliability of the E test for detection of ampicillin, vancomycin, and high-level aminoglycoside resistance in Enterococcus spp","volume":"31","author":"Schulz","year":"1993","journal-title":"J Clin Microbiol"},{"key":"2026070705415051500_dkag227-B15","doi-asserted-by":"publisher","first-page":"2072","DOI":"10.1128\/JCM.02636-05","article-title":"Evaluation of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of staphylococci and enterococci","volume":"44","author":"Carroll","year":"2006","journal-title":"J Clin Microbiol"},{"key":"2026070705415051500_dkag227-B16","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1128\/JCM.02432-13","article-title":"Performance of Vitek 2 for antimicrobial susceptibility testing of Staphylococcus spp. and Enterococcus spp","volume":"52","author":"Bobenchik","year":"2014","journal-title":"J Clin Microbiol"},{"key":"2026070705415051500_dkag227-B17","doi-asserted-by":"publisher","first-page":"3491","DOI":"10.1093\/jac\/dkaa345","article-title":"ResFinder 4.0 for predictions of phenotypes from genotypes","volume":"75","author":"Bortolaia","year":"2020","journal-title":"J Antimicrob Chemother"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/81\/8\/dkag227\/68706071\/dkag227.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/81\/8\/dkag227\/68706071\/dkag227.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T09:41:57Z","timestamp":1783417317000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/doi\/10.1093\/jac\/dkag227\/8725958"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,2]]},"references-count":17,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,7,2]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkag227","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,8]]},"published":{"date-parts":[[2026,7,2]]},"article-number":"dkag227"}}