{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:46:29Z","timestamp":1790142389473,"version":"4.0.1"},"reference-count":7,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"French National Reference Center for Antimicrobial Resistance"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Eravacycline is a novel glycylcycline with broad activity against Gram-negative bacteria, including carbapenem-resistant Enterobacterales. This study aimed to evaluate the performance of gradient diffusion strips and disc diffusion methods for eravacycline susceptibility testing in comparison with a reference broth microdilution method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>A total of 303 carbapenem-non-susceptible Enterobacterales clinical isolates collected at the French National Reference Center were included. Eravacycline susceptibility was determined using broth microdilution (reference method), two gradient diffusion strips (BioM\u00e9rieux Etest\u00ae and Liofilchem) and two different discs (MAST and i2a). Performances were assessed according to ISO 20776-2 guidelines using essential agreement (EA), bias, categorical agreement (CA) and error rates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Eravacycline exhibited lower MICs than tigecycline across all species, with higher susceptibility observed in E. coli. Neither gradient strips nor disc diffusion methods met ISO performance criteria when considering the full collection. However, when only E. coli isolates were considered, Liofilchem gradient strips and both disc diffusion methods achieved acceptable performance, whereas BioM\u00e9rieux Etest\u00ae showed acceptable EA despite high bias.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>While eravacycline shows strong in vitro activity against carbapenem-resistant Enterobacterales, current gradient strip and disc diffusion methods demonstrate limited reliability on Enterobacterales, although good performances were achieved for E. coli. These findings highlight the need for cautious interpretation and further optimization of routine susceptibility testing methods.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkag248","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T11:48:35Z","timestamp":1783511315000},"source":"Crossref","is-referenced-by-count":2,"title":["Comparative evaluation of disc diffusion, Liofilchem\u2122 MTS strips, Etest\u00ae Biom\u00e9rieux with broth microdilution for eravacylin susceptibility testing"],"prefix":"10.1093","volume":"81","author":[{"given":"Sarah","family":"Ronsin","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB\/IDMIT\/UMRS1184) , Fontenay-aux-Roses & Le Kremlin-Bic\u00eatre ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agn\u00e8s B","family":"Jousset","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB\/IDMIT\/UMRS1184) , Fontenay-aux-Roses & Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Bacteriology-Hygiene Unit, Bic\u00eatre Hospital, Assistance Publique-H\u00f4pitaux de Paris , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacterales , Le Kremlin-Bic\u00eatre ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena","family":"Latour","sequence":"additional","affiliation":[{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacterales , Le Kremlin-Bic\u00eatre ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haniel","family":"Defoi","sequence":"additional","affiliation":[{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacterales , Le Kremlin-Bic\u00eatre ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9484-7932","authenticated-orcid":false,"given":"Ines","family":"Rezzoug","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB\/IDMIT\/UMRS1184) , Fontenay-aux-Roses & Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Bacteriology-Hygiene Unit, Bic\u00eatre Hospital, Assistance Publique-H\u00f4pitaux de Paris , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacterales , Le Kremlin-Bic\u00eatre ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Emeraud","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB\/IDMIT\/UMRS1184) , Fontenay-aux-Roses & Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Bacteriology-Hygiene Unit, Bic\u00eatre Hospital, Assistance Publique-H\u00f4pitaux de Paris , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacterales , Le Kremlin-Bic\u00eatre ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9my A","family":"Bonnin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB\/IDMIT\/UMRS1184) , Fontenay-aux-Roses & Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Bacteriology-Hygiene Unit, Bic\u00eatre Hospital, Assistance Publique-H\u00f4pitaux de Paris , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacterales , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"IHU SEPSIS Comprehensive Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7384","authenticated-orcid":false,"given":"Laurent","family":"Dortet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB\/IDMIT\/UMRS1184) , Fontenay-aux-Roses & Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Bacteriology-Hygiene Unit, Bic\u00eatre Hospital, Assistance Publique-H\u00f4pitaux de Paris , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacterales , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"IHU SEPSIS Comprehensive Center"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"key":"2026072415100812200_dkag248-B1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26092671","article-title":"New antibiotics for multidrug-resistant bacterial strains: latest research developments and future perspectives","volume":"26","author":"Terreni","year":"2021","journal-title":"Molecules"},{"key":"2026072415100812200_dkag248-B2","doi-asserted-by":"publisher","first-page":"1575","DOI":"10.1517\/13543784.2014.965253","article-title":"Eravacycline for the treatment of intra-abdominal infections","volume":"23","author":"Bassetti","year":"2014","journal-title":"Expert Opin Investig Drugs"},{"key":"2026072415100812200_dkag248-B3","doi-asserted-by":"publisher","first-page":"3840","DOI":"10.1128\/AAC.00436-16","article-title":"In vitro activity of eravacycline against carbapenem-resistant Enterobacteriaceae and Acinetobacter baumannii","volume":"60","author":"Livermore","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"2026072415100812200_dkag248-B4","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens14121214","article-title":"Resistance of Gram-negative bacteria to eravacycline: a systematic review of data from in vitro studies","volume":"14","author":"Falagas","year":"2025","journal-title":"Pathogens"},{"key":"2026072415100812200_dkag248-B5","doi-asserted-by":"publisher","first-page":"16581","DOI":"10.1038\/s41598-025-98206-6","article-title":"Emergence of transferable tigecycline and eravacycline resistance gene tet(X4) in Escherichia coli isolates from Iran","volume":"15","author":"Haeili","year":"2025","journal-title":"Sci Rep"},{"key":"2026072415100812200_dkag248-B6","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01697-16","article-title":"Chromosomal amplification of the blaOXA-58 carbapenemase gene in a Proteus mirabilis clinical isolate","volume":"61","author":"Girlich","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"key":"2026072415100812200_dkag248-B7","doi-asserted-by":"publisher","first-page":"2771","DOI":"10.1007\/s10096-025-05235-0","article-title":"Eravacycline: evaluation of susceptibility testing methods and activity against multidrug-resistant Enterobacterales and Acinetobacter","volume":"44","author":"Kinet-Poleur","year":"2025","journal-title":"Eur J Clin Microbiol Infect Dis"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/81\/8\/dkag248\/69969152\/dkag248.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/81\/8\/dkag248\/69969152\/dkag248.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T19:10:11Z","timestamp":1784920211000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/doi\/10.1093\/jac\/dkag248\/8741626"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,2]]},"references-count":7,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,7,2]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkag248","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,8]]},"published":{"date-parts":[[2026,7,2]]},"article-number":"dkag248"}}