{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T16:03:09Z","timestamp":1784995389257,"version":"3.55.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"8","funder":[{"DOI":"10.13039\/100015226","name":"Royal Veterinary College","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015226","id-type":"DOI","asserted-by":"publisher"}]},{"name":"London Interdisciplinary Biosciences Consortium Doctoral Training Partnership","award":["BB\/T008709\/1"],"award-info":[{"award-number":["BB\/T008709\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Mobile colistin resistance determinants (mcr) in ESBL-producing Enterobacterales represent a public health concern, given the renewed reliance on colistin as a last-resort agent for multidrug-resistant Gram-negative infections.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To resolve the genetic context and transferability of colistin resistance in an ESBL-producing Enterobacter mori isolate (SH93I1) recovered from seafood (MIC of 8\u2005mg\/L).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We characterized the location, genetic environment and mobility of the colistin resistance determinant in SH93I1 using hybrid whole-genome sequencing (short- and long-read data), comparative genomics, in vitro conjugation to Escherichia coli MG1655 and gene cloning in E. coli DH5\u03b1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>E. mori SH93I1 carried a novel mcr allele, designated mcr-11.1 according to current classification criteria. The allele shared 91% nucleotide identity and 93% predicted amino acid identity with mcr-9.1. mcr-11.1 was located on a 328\u2005kb non-typeable conjugative plasmid that also carried blaCTX\u2212M\u221214. The mcr-11.1 region formed a 25.5\u2005kb segment bracketed by IS3-family elements and included wbuC\u2013qseBC\u2013vapBC and the arnBCADTEF operon. Conjugation experiments confirmed plasmid transfer, with co-transfer of resistance phenotypes; however, colistin MICs were not increased in the E. coli MG1655 transconjugants or in E. coli DH5\u03b1 strains expressing mcr-11.1, alone or with wbuC and qseBC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We describe mcr-11.1 on a large conjugative plasmid in E. mori from seafood, with evidence consistent with mobilization of a chromosomal fragment from L. adecarboxylata. Although mcr-11.1 did not increase colistin MICs in E. coli, its presence on a multidrug-resistance plasmid highlights the potential for dissemination under co-selection and future adaptation in clinically relevant hosts.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkag251","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T11:58:59Z","timestamp":1783684739000},"source":"Crossref","is-referenced-by-count":0,"title":["Identification of novel plasmid-mediated colistin resistance gene\n                    <i>mcr-11.1<\/i>\n                    in\n                    <i>Enterobacter mori<\/i>\n                    isolated from seafood"],"prefix":"10.1093","volume":"81","author":[{"given":"Ryan","family":"Pearce","sequence":"first","affiliation":[{"name":"Department of Tropical Disease Biology, The Liverpool School of Tropical Medicine , Liverpool L3 5QA ,","place":["UK"]},{"name":"Department of Veterinary and Animal Sciences, 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