{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T16:27:42Z","timestamp":1786724862216,"version":"build-2736575974"},"reference-count":10,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":10,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MOLSID"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and objectives<\/jats:title>\n                    <jats:p>Accurate detection of staphylococcal beta-lactamase remains a diagnostic challenge, despite its importance for guiding antibiotic therapy, particularly in severe infections, such as endocarditis and bacteraemia. Currently, no available method reliably detects beta-lactamase activity in all staphylococcal species. Here, we evaluated a novel fluorogenic biotracer, 352600-C (MOLSID, Lyon, France), designed to detect beta-lactamase activity in staphylococci.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The 352600-C biotracer was incorporated into Mueller\u2013Hinton agar plates onto which staphylococcal strains were subcultured at 37\u00b0C. Colony fluorescence was assessed at 4, 6 and 24\u2005h using a 365\u2005nm UV lamp. The assay was performed on a large collection of blaZ-positive (n\u200a=\u200a124, including BlaZ types A, B, C and D) and blaZ-negative (n\u200a=\u200a69) staphylococci from 18 species (Staphylococcus aureus, n\u200a=\u200a100; S. non-aureus, n\u200a=\u200a93). Molecular detection of the blaZ gene using WGS served as the gold standard. Phenotypic susceptibility to penicillins was also tested using EUCAST recommendations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>After 4, 6 and 24\u2005h of incubation, the assay showed sensitivities of 92.2%, 96.9% and 100% for S. aureus and 50.0%, 88.3% and 100% for non-aureus staphylococci, respectively. None of the 69 blaZ-negative strains produced fluorescent colonies (specificity 100%). Notably, the assay detected all BlaZ types. Penicillin disc diffusion showed 98.4% sensitivity and 77.8% specificity for MSSA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This novel assay for the detection of staphylococcal beta-lactamase demonstrated simplicity, rapidity and excellent accuracy after 24\u2005h incubation (100% sensitivity and specificity) across a genetically diverse and representative collection of clinical isolates, making it a valuable tool for routine diagnostics and antimicrobial stewardship.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkag268","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T11:51:43Z","timestamp":1786535503000},"source":"Crossref","is-referenced-by-count":0,"title":["A fluorogenic biotracer for reliable detection of staphylococcal beta-lactamase: towards improved precision therapy in severe MSSA infections"],"prefix":"10.1093","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7388-4087","authenticated-orcid":false,"given":"C\u00e9line","family":"Dupieux","sequence":"first","affiliation":[{"name":"French National Reference Centre for Staphylococci, Institut des Agents Infectieux, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Centre for Integrative Research in Infectious Diseases and Immunology, Inserm U1111, CNRS UMR5308, ENS de Lyon, Universit\u00e9 Lyon 1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Martins Simoes","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Staphylococci, Institut des Agents Infectieux, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Centre for Integrative Research in Infectious Diseases and Immunology, Inserm U1111, CNRS UMR5308, ENS de Lyon, Universit\u00e9 Lyon 1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6282-6498","authenticated-orcid":false,"given":"Benjamin","family":"Youenou","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Staphylococci, Institut des Agents Infectieux, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Centre for Integrative Research in Infectious Diseases and Immunology, Inserm U1111, CNRS UMR5308, ENS de Lyon, Universit\u00e9 Lyon 1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Vandenesch","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Staphylococci, Institut des Agents Infectieux, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Centre for Integrative Research in Infectious Diseases and Immunology, Inserm U1111, CNRS UMR5308, ENS de Lyon, Universit\u00e9 Lyon 1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9471-2655","authenticated-orcid":false,"given":"Anne","family":"Tristan","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Staphylococci, Institut des Agents Infectieux, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Centre for Integrative Research in Infectious Diseases and Immunology, Inserm U1111, CNRS UMR5308, ENS de Lyon, Universit\u00e9 Lyon 1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Laurent","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Staphylococci, Institut des Agents Infectieux, Hospices Civils de Lyon , Lyon ,","place":["France"]},{"name":"Centre for Integrative Research in Infectious Diseases and Immunology, Inserm U1111, CNRS UMR5308, ENS de Lyon, Universit\u00e9 Lyon 1 , Lyon ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"2026081411010241300_dkag268-B1","doi-asserted-by":"publisher","first-page":"3731","DOI":"10.1128\/AAC.00101-07","article-title":"Impact of empirical-therapy selection on outcomes of intravenous drug users with infective endocarditis caused by methicillin-susceptible Staphylococcus aureus","volume":"51","author":"Lodise","year":"2007","journal-title":"Antimicrob Agents Chemother"},{"key":"2026081411010241300_dkag268-B2","doi-asserted-by":"publisher","first-page":"1589","DOI":"10.1097\/01.CCM.0000217961.75225.E9","article-title":"Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock","volume":"34","author":"Kumar","year":"2006","journal-title":"Crit Care Med"},{"key":"2026081411010241300_dkag268-B3","doi-asserted-by":"publisher","first-page":"1418","DOI":"10.1086\/375057","article-title":"Outcomes analysis of delayed antibiotic treatment for hospital-acquired Staphylococcus aureus bacteremia","volume":"36","author":"Lodise","year":"2003","journal-title":"Clin Infect Dis"},{"key":"2026081411010241300_dkag268-B4","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1093\/jac\/dkw521","article-title":"Comparison of phenotypic methods for the detection of penicillinase in Staphylococcus aureus and proposal of a practical diagnostic approach","volume":"72","author":"Hombach","year":"2017","journal-title":"J Antimicrob Chemother"},{"key":"2026081411010241300_dkag268-B5","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/j.jinf.2016.05.011","article-title":"The prevalence, population structure and screening test specificity of penicillin-susceptible Staphylococcus aureus bacteremia isolates in Malm\u00f6, Sweden","volume":"73","author":"Resman","year":"2016","journal-title":"J Infect"},{"key":"2026081411010241300_dkag268-B6","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1080\/23744235.2017.1405276","article-title":"Trends in incidence and resistance patterns of Staphylococcus aureus bacteremia","volume":"50","author":"Jokinen","year":"2018","journal-title":"Infect Dis (Lond)"},{"key":"2026081411010241300_dkag268-B7","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2024.51353","article-title":"\u03b2-lactam inoculum effect in methicillin-susceptible Staphylococcus aureus infective endocarditis","volume":"7","author":"Jean","year":"2024","journal-title":"JAMA Netw Open"},{"key":"2026081411010241300_dkag268-B8","author":"Tong S, for the S. aureus Network Adaptive Platform Trial Group"},{"key":"2026081411010241300_dkag268-B9","doi-asserted-by":"publisher","DOI":"10.1002\/anse.202500160","article-title":"Simplicity and reliability achieved in antimicrobial resistance testing through transformative design of a \u03b2-lactamase-responsive molecular probe","volume":"6","author":"Verrier","year":"2025","journal-title":"Anal Sens"},{"key":"2026081411010241300_dkag268-B10","author":"The European Committee on Antimicrobial Susceptibility Testing"}],"container-title":["Journal of Antimicrobial 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