{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:26:34Z","timestamp":1787127994797,"version":"build-2736575974"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":15,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019721","name":"Roche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019721","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Vingroup Innovation Foundation","award":["VINIF.2023.ThS.140"],"award-info":[{"award-number":["VINIF.2023.ThS.140"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>HIV drug resistance (HIVDR) threatens the effectiveness of antiretroviral therapy (ART) programmes in low- and middle-income countries. The Cobas Plasma Separation Card (PSC) is a recently developed specimen collection tool with potential to expand access to HIVDR genotyping in remote settings. This study evaluates PSC performance for HIVDR genotyping compared with plasma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Paired plasma and PSC specimens were selected from 250 people living with HIV (PLHIV) on ART \u22656\u2005months with viral load (VL) \u22651000\u2005copies\/mL. HIVDR genotyping followed CDC Atlanta in-house protocol; resistance interpretation used the Stanford HIVdb algorithm. Paired sequences were compared for subtype, nucleotide similarity, mutation profile and drug-resistance classification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Twenty paired specimens met inclusion criteria. Amplification of the HIV-1 Pol gene succeeded in 19\/20 (95%) PSC samples. Of 19 paired sequences, nucleotide similarity was 98.5% (IQR 98.0%\u201399.3%). Eleven plasma samples showed 37 major drug-resistance mutations (MDM); 35 (94.6%) concordant MDM were identified in PSC. Discordances in four pairs were limited to mixed versus single bases and did not alter drug-resistance classification. Only one pair showed a clinically significant discordance (K103N detected in plasma but not initially in PSC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>PSC demonstrated good concordance with plasma for HIVDR genotyping and has potential as an alternative specimen type for clinical practice and surveillance in resource-limited settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkag279","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T11:44:35Z","timestamp":1785843875000},"source":"Crossref","is-referenced-by-count":0,"title":["Performance of plasma separation cards for HIV drug-resistance genotyping in a resource-limited setting: comparison with plasma samples"],"prefix":"10.1093","volume":"81","author":[{"given":"Thang Hong","family":"Pham","sequence":"first","affiliation":[{"name":"National Institute of Hygiene and Epidemiology (NIHE) , Hanoi 100000 ,","place":["Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5188-4043","authenticated-orcid":false,"given":"Hanh Thi Hong","family":"Ngo","sequence":"additional","affiliation":[{"name":"National Institute of Hygiene and Epidemiology (NIHE) , Hanoi 100000 ,","place":["Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tram Thi Phuong","family":"Pham","sequence":"additional","affiliation":[{"name":"National Institute of Hygiene and Epidemiology (NIHE) , Hanoi 100000 ,","place":["Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nhung Thi Hong","family":"Le","sequence":"additional","affiliation":[{"name":"National Institute of Hygiene and Epidemiology (NIHE) , Hanoi 100000 ,","place":["Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai Thi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Vietnam Administration of Disease Prevention (VADP) , Hanoi ,","place":["Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuan Anh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"National Institute of Hygiene and Epidemiology (NIHE) , Hanoi 100000 ,","place":["Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huyen Thi","family":"Do","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Vietnam Academy of Science and Technology , Hanoi ,","place":["Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duong Nhu","family":"Tran","sequence":"additional","affiliation":[{"name":"National Institute of Hygiene and Epidemiology (NIHE) , Hanoi 100000 ,","place":["Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan Trong","family":"Phan","sequence":"additional","affiliation":[{"name":"National Institute of Hygiene and Epidemiology (NIHE) , Hanoi 100000 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