{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:26:42Z","timestamp":1787128002181,"version":"build-2736575974"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":15,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Canisius-Wilhelmina Hospital","award":["CWZ_001421"],"award-info":[{"award-number":["CWZ_001421"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sporotrichosis caused by Sporothrix brasiliensis is increasingly reported in South America. Infections in humans are mostly due to zoonotic transmission by cats. For both hosts, itraconazole is the drug of choice despite long treatment duration. Resistance surveillance is warranted, but protocols for antifungal susceptibility testing (AFST) are not always harmonized between laboratories largely due to Sporothrix species being thermally dimorphic and the absence of well-defined consensus methodologies to address how to deal with yeast\/mould phases.<\/jats:p>\n                  <jats:p>To standardize susceptibility testing methodology, we propose to modify the current Clinical Laboratory and Standards Institute (CLSI) M38 broth microdilution methodology with S. brasiliensis culture for 7 days at 30\u00b0C (rather than 35\u00b0C) on potato dextrose agar (PDA), followed by microscopic confirmation of pure mycelial phase. Incubation temperature would also be modified to 30\u00b0C for 72\u2005hours. By following this standardized method for at least itraconazole, terbinafine and amphotericin B, S. brasiliensis non-wild-type isolates and correlations with clinical outcomes can be properly investigated. The proposed method is a consensus-oriented interim approach rather than a fully validated standard.<\/jats:p>","DOI":"10.1093\/jac\/dkag286","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T11:49:50Z","timestamp":1786016990000},"source":"Crossref","is-referenced-by-count":0,"title":["Towards standardized antifungal susceptibility testing for\n                    <i>Sporothrix brasiliensis<\/i>"],"prefix":"10.1093","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6734-361X","authenticated-orcid":false,"given":"Bram","family":"Spruijtenburg","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc , Nijmegen ,","place":["The Netherlands"]},{"name":"Radboudumc-CWZ Center of Expertise for Mycology , Nijmegen ,","place":["The Netherlands"]},{"name":"Department of Medical Microbiology and Immunology, Canisius-Wilhelmina Hospital (CWZ)\/Dicoon , Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Melchior Do Prado","sequence":"additional","affiliation":[{"name":"Radboudumc-CWZ Center of Expertise for Mycology , Nijmegen ,","place":["The Netherlands"]},{"name":"Department of Medical Microbiology and Immunology, Canisius-Wilhelmina Hospital (CWZ)\/Dicoon , Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Rodrigues Tr\u00e1paga","sequence":"additional","affiliation":[{"name":"Post-graduation Program in Health Science, Faculty of Medicine (FAMED), Federal University of Rio Grande (FURG) , Rio Grande ,","place":["Brazil"]},{"name":"FAMED-FURG Mycology Laboratory, , Rio Grande ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5707-528X","authenticated-orcid":false,"given":"Dallas J","family":"Smith","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention , Atlanta , ,","place":["Georgia USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5871-3933","authenticated-orcid":false,"given":"Philippe J","family":"Dufresne","sequence":"additional","affiliation":[{"name":"Laboratoire de Sante\u0301 Publique du Qu\u00e9bec, Institut National de Sant\u00e9 Publique du Qu\u00e9bec, Sainte-Anne-de-Bellevue , Qu\u00e9bec ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3253-6080","authenticated-orcid":false,"given":"Jacques F","family":"Meis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc , Nijmegen ,","place":["The Netherlands"]},{"name":"Radboudumc-CWZ Center of Expertise for Mycology , Nijmegen ,","place":["The Netherlands"]},{"name":"Institute of Translational Research, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) and Excellence Center for Medical Mycology, University of Cologne , Cologne ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Faculty of Ceil\u00e2ndia, University of Bras\u00edlia ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Sueli","family":"Felipe","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Genomic Sciences and Biotechnology, Universidade Cat\u00f3lica de Bras\u00edlia , Bras\u00edlia ,","place":["Brazil"]},{"name":"Universidade de Bras\u00edlia , Bras\u00edlia ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7597-602X","authenticated-orcid":false,"given":"Daniel R","family":"Matute","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina at Chapel Hill , Chapel Hill, NC ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theun","family":"De Groot","sequence":"additional","affiliation":[{"name":"Radboudumc-CWZ Center of Expertise for Mycology , Nijmegen ,","place":["The Netherlands"]},{"name":"Department of Medical Microbiology and Immunology, Canisius-Wilhelmina Hospital (CWZ)\/Dicoon , Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Ribeiro","family":"Dos Santos","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, S\u00e3o Paulo University (USP) , S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regielly Caroline Raimundo","family":"Cognialli","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Microbiology, Parasitology and Pathology, Biological Sciences, Department of Basic Pathology, Federal University of Paran\u00e1 , Curitiba ,","place":["Brazil"]},{"name":"Hospital de Cl\u00ednicas, Federal University of Paran\u00e1 , Curitiba ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2225-5122","authenticated-orcid":false,"given":"Nathan P","family":"Wiederhold","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio , San Antonio, TX ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e2nia A","family":"Vicente","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Microbiology, Parasitology and Pathology, Biological Sciences, Department of Basic Pathology, Federal University of Paran\u00e1 , Curitiba ,","place":["Brazil"]},{"name":"Microbiology Collections Center of Paran\u00e1 Network-Taxonline (CMRP\/Taxonline), Department of Basic Pathology, Federal University of Paran\u00e1 , Curitiba ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio de","family":"Queiroz-Telles","sequence":"additional","affiliation":[{"name":"Department of Public Health, Hospital de Cl\u00ednicas, Federal University of Paran\u00e1 , Curitiba ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3883-0080","authenticated-orcid":false,"given":"Melissa Orzechowski","family":"Xavier","sequence":"additional","affiliation":[{"name":"Post-graduation Program in Health Science, Faculty of Medicine (FAMED), Federal University of Rio Grande (FURG) , Rio Grande ,","place":["Brazil"]},{"name":"FAMED-FURG Mycology Laboratory, , Rio Grande 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Chemother"},{"key":"2026081903551424500_dkag286-B7","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi Third Edition: M38","author":"CLSI","year":"2017"},{"key":"2026081903551424500_dkag286-B8","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1093\/mmy\/myw143","article-title":"In vitro susceptibility of Sporothrix brasiliensis: comparison of yeast and mycelial phases","volume":"55","author":"Sanchotene","year":"2017","journal-title":"Med Mycol"},{"key":"2026081903551424500_dkag286-B9","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01907-25","article-title":"Establishment of epidemiological cutoff values for clinically relevant Sporothrix species using CLSI-broth microdilution","volume":"70","author":"dos Santos","year":"2026","journal-title":"Antimicrob Agents Chemother"},{"key":"2026081903551424500_dkag286-B10","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1111\/myc.13584","article-title":"Genotyping 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