{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:45:25Z","timestamp":1792053925736,"version":"4.4.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100018592","name":"fifth phase of the \u2018333 Project\u2019 scientific research project in Jiangsu Province","doi-asserted-by":"publisher","award":["BRA2020002"],"award-info":[{"award-number":["BRA2020002"]}],"id":[{"id":"10.13039\/501100018592","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institution","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Postgraduate Research & Practice Innovation Program of Jiangsu Province","award":["SJCX23_1958"],"award-info":[{"award-number":["SJCX23_1958"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Carbapenem-resistant Enterobacterales (CRE), particularly those carrying New Delhi metallo-\u03b2-lactamase (NDM) variants, pose a major global health threat. Among these, NDM-7 has attracted increasing attention due to its enhanced carbapenemase activity and clinical relevance. This study investigated the prevalence, genomic characteristics and dissemination of blaNDM-7-positive Klebsiella pneumoniae complex isolates from clinical and environmental sources in Yangzhou, China.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 401 clinical isolates, 282 hospital surface swabs and 366 wastewater samples collected between 2018 and 2024 were screened for blaNDM. Antimicrobial susceptibility testing and whole-genome sequencing were performed to characterize resistance profiles, genomic features and phylogenetic relationships. Conjugation and serial-passage assays were used to assess plasmid transferability and stability, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Seven blaNDM-7-positive isolates were identified, including one clinical isolate, one hospital environmental isolate and five wastewater isolates. All exhibited multidrug resistance. The blaNDM-7 gene was located on highly conserved \u223c46\u2005kb IncX3 plasmids that exhibited high conjugation frequencies (10\u22125-10\u22124) and stability (96.0%\u201398.7% mean retention after approximately 600 generations). Phylogenetic analysis revealed close genomic relatedness among the six ST196 K. quasipneumoniae isolates recovered from different sources, with only 0-7 core-genome SNP differences. All six isolates were recovered within approximately 6\u2005months, consistent with recent common ancestry and possible clonal dissemination across settings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The close genomic relatedness of ST196 isolates from different sources supports possible clonal dissemination, while the high conservation, transferability and stability of IncX3 plasmids highlight their important role in the dissemination of blaNDM-7. These findings underscore the importance of continued genomic surveillance of blaNDM-7 and its associated mobile genetic elements across clinical and environmental settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkag346","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:58:44Z","timestamp":1790769524000},"source":"Crossref","is-referenced-by-count":0,"title":["Genomic evidence of clonal and IncX3 plasmid-mediated dissemination of\n                    <i>bla<\/i>\n                    NDM-7 in the\n                    <i>Klebsiella pneumoniae<\/i>\n                    complex"],"prefix":"10.1093","volume":"81","author":[{"given":"Yujing","family":"Zhang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis, Yangzhou University , Yangzhou ,","place":["China"]},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University , Yangzhou ,","place":["China"]},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of China, Yangzhou University , Yangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guqi","family":"Ren","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis, Yangzhou University , Yangzhou ,","place":["China"]},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University , Yangzhou ,","place":["China"]},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of China, Yangzhou University , Yangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"Yangzhou Jieyuan Environment Co., LTD. 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University , Yangzhou ,","place":["China"]},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of China, Yangzhou University , Yangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis, Yangzhou University , Yangzhou ,","place":["China"]},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University , Yangzhou ,","place":["China"]},{"name":"Institute of Medical Sciences, School of Public Health, Xinjiang Medical University , Urumqi ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinan","family":"Jiao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis, Yangzhou University , Yangzhou 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