{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:11:47Z","timestamp":1791130307523,"version":"4.1.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,6,1]]},"DOI":"10.1093\/jac\/dkl426","type":"journal-article","created":{"date-parts":[[2006,10,25]],"date-time":"2006-10-25T02:28:54Z","timestamp":1161743334000},"page":"1216-1222","source":"Crossref","is-referenced-by-count":86,"title":["Altered spectrum of multidrug resistance associated with a single point mutation in the Escherichia coli RND-type MDR efflux pump YhiV (MdtF)"],"prefix":"10.1093","volume":"59","author":[{"given":"J\u00fcrgen A.","family":"Bohnert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Schuster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"F\u00e4hnrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer","family":"Trittler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winfried V.","family":"Kern","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,10,24]]},"reference":[{"key":"key\n\t\t\t\t20171012174608_b1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2165\/00003495-200464020-00004","article-title":"Efflux-mediated drug resistance in bacteria","volume":"64","author":"Li","year":"2004","journal-title":"Drugs"},{"key":"key\n\t\t\t\t20171012174608_b2","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1128\/CMR.19.2.382-402.2006","article-title":"Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria","volume":"19","author":"Piddock","year":"2006","journal-title":"Clin Microbiol Rev"},{"key":"key\n\t\t\t\t20171012174608_b3","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1093\/jac\/dki171","article-title":"Efflux-mediated antimicrobial resistance","volume":"56","author":"Poole","year":"2005","journal-title":"J Antimicrob Chemother"},{"key":"key\n\t\t\t\t20171012174608_b4","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1128\/AAC.44.4.814-820.2000","article-title":"Non-target gene mutations in the development of fluoroquinolone resistance in Escherichia coli","volume":"44","author":"Kern","year":"2000","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20171012174608_b5","doi-asserted-by":"crossref","first-page":"3441","DOI":"10.1128\/AAC.44.12.3441-3443.2000","article-title":"High-level fluoroquinolone-resistant clinical isolates of Escherichia coli overproduce multidrug efflux protein AcrA","volume":"44","author":"Mazzariol","year":"2000","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20171012174608_b6","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1128\/AAC.45.5.1515-1521.2001","article-title":"Genetic characterization of highly fluoroquinolone-resistant clinical Escherichia coli strains from China: role of acrR mutations","volume":"45","author":"Wang","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20171012174608_b7","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1128\/AAC.45.5.1467-1472.2001","article-title":"Enhanced expression of the multidrug efflux pumps AcrAB and AcrEF associated with insertion element transposition in Escherichia coli mutants selected with a fluoroquinolone","volume":"45","author":"Jellen-Ritter","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20171012174608_b8","doi-asserted-by":"crossref","first-page":"4390","DOI":"10.1128\/AAC.49.10.4390-4392.2005","article-title":"Contribution of mutation at amino acid 45 of AcrR to acrB expression and ciprofloxacin resistance in clinical and veterinary Escherichia coli isolates","volume":"49","author":"Webber","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20171012174608_b9","doi-asserted-by":"crossref","first-page":"7815","DOI":"10.1128\/JB.187.22.7815-7825.2005","article-title":"Cell division defects in Escherichia coli deficient in the multidrug efflux transporter AcrEF-TolC","volume":"187","author":"Lau","year":"2005","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b10","doi-asserted-by":"crossref","first-page":"4168","DOI":"10.1128\/JB.184.15.4168-4176.2002","article-title":"The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate","volume":"184","author":"Baranova","year":"2002","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b11","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1093\/jac\/dkg406","article-title":"Beta-lactam resistance modulated by the overexpression of response regulators of two-component signal transduction systems in Escherichia coli","volume":"52","author":"Hirakawa","year":"2003","journal-title":"J Antimicrob Chemother"},{"key":"key\n\t\t\t\t20171012174608_b12","doi-asserted-by":"crossref","first-page":"4161","DOI":"10.1128\/JB.184.15.4161-4167.2002","article-title":"The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC","volume":"184","author":"Nagakubo","year":"2002","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b13","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1128\/JB.184.8.2319-2323.2002","article-title":"EvgA of the two-component signal transduction system modulates production of the YhiUV multidrug transporter in Escherichia coli","volume":"184","author":"Nishino","year":"2002","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b14","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1128\/JB.188.3.1191-1195.2006","article-title":"Mammalian steroid hormones are substrates for the major RND- and MFS-type tripartite multidrug efflux pumps of Escherichia coli","volume":"188","author":"Elkins","year":"2006","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b15","doi-asserted-by":"crossref","first-page":"6225","DOI":"10.1128\/JB.184.22.6225-6234.2002","article-title":"Escherichia coli gene expression responsive to levels of the response regulator EvgA","volume":"184","author":"Masuda","year":"2002","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b16","doi-asserted-by":"crossref","first-page":"2667","DOI":"10.1128\/JB.185.8.2667-2672.2003","article-title":"Global analysis of genes regulated by EvgA of the two-component regulatory system in Escherichia coli","volume":"185","author":"Nishino","year":"2003","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b17","doi-asserted-by":"crossref","first-page":"6701","DOI":"10.1128\/JB.187.19.6701-6707.2005","article-title":"TolC is involved in enterobactin efflux across the outer membrane of Escherichia coli","volume":"187","author":"Bleuel","year":"2005","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b18","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1093\/jac\/dki445","article-title":"Effect of 1-(1-naphthylmethyl)-piperazine, a novel putative efflux pump inhibitor, on antimicrobial drug susceptibility in clinical isolates of Escherichia coli","volume":"57","author":"Kern","year":"2006","journal-title":"J Antimicrob Chemother"},{"key":"key\n\t\t\t\t20171012174608_b19","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/S0378-4347(01)00115-3","article-title":"A new and rapid method for monitoring the new oxazolidinone antibiotic linezolid in serum and urine by high performance liquid chromatography-integrated sample preparation","volume":"755","author":"Ehrlich","year":"2001","journal-title":"J Chromatogr B Biomed Sci Appl"},{"key":"key\n\t\t\t\t20171012174608_b20","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/AAC.44.1.10-13.2000","article-title":"Ineffectiveness of topoisomerase mutations in mediating clinically significant fluoroquinolone resistance in Escherichia coli in the absence of the AcrAB efflux pump","volume":"44","author":"Oethinger","year":"2000","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20171012174608_b21","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1093\/nar\/27.6.1555","article-title":"Rapid modification of bacterial artificial chromosomes by ET-recombination","volume":"27","author":"Muyrers","year":"1999","journal-title":"Nucleic Acids Res"},{"key":"key\n\t\t\t\t20171012174608_b22","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/2417","article-title":"A new logic for DNA engineering using recombination in Escherichia coli","volume":"20","author":"Zhang","year":"1998","journal-title":"Nature Genet"},{"key":"key\n\t\t\t\t20171012174608_b23","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.1128\/AEM.64.8.2763-2769.1998","article-title":"Use of the lactococcal nisA promoter to regulate gene expression in Gram-positive bacteria: comparison of induction level and promoter strength","volume":"64","author":"Eichenbaum","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"key\n\t\t\t\t20171012174608_b24","doi-asserted-by":"crossref","first-page":"e36","DOI":"10.1093\/nar\/30.9.e36","article-title":"Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR","volume":"30","author":"Pfaffl","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"key\n\t\t\t\t20171012174608_b25","doi-asserted-by":"crossref","first-page":"3381","DOI":"10.1093\/nar\/gkg520","article-title":"SWISS-MODEL: An automated protein homology-modeling server","volume":"31","author":"Schwede","year":"2003","journal-title":"Nucleic Acids Res"},{"key":"key\n\t\t\t\t20171012174608_b26","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nature01050","article-title":"Crystal structure of the bacterial multidrug efflux transporter AcrB","volume":"419","author":"Murakami","year":"2002","journal-title":"Nature"},{"key":"key\n\t\t\t\t20171012174608_b27","doi-asserted-by":"crossref","first-page":"4479","DOI":"10.1093\/nar\/19.16.4479","article-title":"Molecular characterization of the soxRS genes of Escherichia coli: two genes control a superoxide stress regulon","volume":"19","author":"Amabile-Cuevas","year":"1991","journal-title":"Nucleic Acids Res"},{"key":"key\n\t\t\t\t20171012174608_b28","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1128\/AAC.49.2.849-852.2005","article-title":"Selected arylpiperazines are capable of reversing multidrug resistance in Escherichia coli overexpressing RND efflux pumps","volume":"49","author":"Bohnert","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20171012174608_b29","doi-asserted-by":"crossref","first-page":"5803","DOI":"10.1128\/JB.183.20.5803-5812.2001","article-title":"Analysis of a complete library of putative drug transporter genes in Escherichia coli","volume":"183","author":"Nishino","year":"2001","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b30","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1016\/j.febslet.2006.01.024","article-title":"Insight in eukaryotic ABC transporter function by mutation analysis","volume":"580","author":"Frelet","year":"2006","journal-title":"FEBS Lett"},{"key":"key\n\t\t\t\t20171012174608_b31","doi-asserted-by":"crossref","first-page":"3477","DOI":"10.1128\/JB.180.13.3477-3479.1998","article-title":"Characterization of the earliest known Staphylococcus aureus plasmid encoding a multidrug efflux system","volume":"180","author":"Paulsen","year":"1998","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b32","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.1128\/jb.179.7.2189-2193.1997","article-title":"Mutations affecting substrate specificity of the Bacillus subtilis multidrug transporter Bmr","volume":"179","author":"Klyachko","year":"1997","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b33","doi-asserted-by":"crossref","first-page":"8957","DOI":"10.1074\/jbc.M313422200","article-title":"Determinants of substrate recognition by the Escherichia coli multidrug transporter MdfA identified on both sides of the membrane","volume":"279","author":"Adler","year":"2004","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20171012174608_b34","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.bbabio.2004.06.004","article-title":"Energetics of wild-type and mutant multidrug resistance secondary transporter LmrP of Lactococcus lactis","volume":"1658","author":"Mazurkiewicz","year":"2004","journal-title":"Biochim Biophys Acta"},{"key":"key\n\t\t\t\t20171012174608_b35","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1126\/science.1083137","article-title":"Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump","volume":"300","author":"Yu","year":"2003","journal-title":"Science"},{"key":"key\n\t\t\t\t20171012174608_b36","doi-asserted-by":"crossref","first-page":"6804","DOI":"10.1128\/JB.187.19.6804-6815.2005","article-title":"A periplasmic drug-binding site of the AcrB multidrug efflux pump: a crystallographic and site-directed mutagenesis study","volume":"187","author":"Yu","year":"2005","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b37","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1093\/oxfordjournals.jbchem.a003069","article-title":"Membrane topology of a multidrug efflux transporter, AcrB, in Escherichia coli","volume":"131","author":"Fujihira","year":"2002","journal-title":"J Biochem (Tokyo)"},{"key":"key\n\t\t\t\t20171012174608_b38","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/JB.188.1.115-123.2006","article-title":"Mutations in the central cavity and periplasmic domain affect efflux activity of the resistance-nodulation-division pump EmhB from Pseudomonas fluorescens cLP6a","volume":"188","author":"Hearn","year":"2006","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b39","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1046\/j.1365-2958.2002.03223.x","article-title":"On the mechanism of substrate specificity by resistance nodulation division (RND)-type multidrug resistance pumps: the large periplasmic loops of MexD from Pseudomonas aeruginosa are involved in substrate recognition","volume":"46","author":"Mao","year":"2002","journal-title":"Mol Microbiol"},{"key":"key\n\t\t\t\t20171012174608_b40","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1128\/JB.186.5.1258-1269.2004","article-title":"Differential impact of MexB mutations on substrate selectivity of the MexAB-OprM multidrug efflux pump of Pseudomonas aeruginosa","volume":"186","author":"Middlemiss","year":"2004","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20171012174608_b41","doi-asserted-by":"crossref","first-page":"3743","DOI":"10.1074\/jbc.M308893200","article-title":"Extramembrane central pore of multidrug exporter AcrB in Escherichia coli plays an important role in drug transport","volume":"279","author":"Murakami","year":"2004","journal-title":"J Biol Chem"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jac\/article-pdf\/59\/6\/1216\/2090868\/dkl426.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,18]],"date-time":"2020-04-18T19:14:33Z","timestamp":1587237273000},"score":1,"resource":{"primary":{"URL":"http:\/\/academic.oup.com\/jac\/article\/59\/6\/1216\/709259\/Altered-spectrum-of-multidrug-resistance"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,24]]},"references-count":41,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2006,10,24]]},"published-print":{"date-parts":[[2007,6,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkl426","relation":{},"ISSN":["1460-2091","0305-7453"],"issn-type":[{"value":"1460-2091","type":"electronic"},{"value":"0305-7453","type":"print"}],"subject":[],"published-other":{"date-parts":[[2007,6]]},"published":{"date-parts":[[2006,10,24]]}}}