{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:09:59Z","timestamp":1773007799113,"version":"3.50.1"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The objective was to study a new vanG-type locus in Clostridium argentinense vanGCar and to determine its impact on glycopeptide susceptibility of the host.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The whole genome of C. argentinense NCIB 10714 was sequenced using Illumina single-reads sequencing technology. The presence of vanGCar in seven C. argentinense strains was tested by PCR and its expression was tested by quantitative RT\u2013PCR (qRT\u2013PCR). Glycopeptide susceptibility was determined by the Etest procedure.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The vanGCar locus contained four genes encoding a carboxypeptidase, a d-alanine:d-serine ligase, a serine transporter and a serine racemase, and was present in the seven C. argentinense studied. An AraC-type transcriptional regulator was found upstream from the genes. C. argentinense NCIB 10714 was susceptible to vancomycin and to teicoplanin. qRT\u2013PCR experiments revealed that vanGCar was not expressed without or with induction by a subinhibitory concentration of vancomycin.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The new vanGCar locus was cryptic in C. argentinense and intrinsic to this species. Emergence of vancomycin resistance in C. argentinense due to decryptification of the vanGCar gene cluster could occur.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkv073","type":"journal-article","created":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T20:19:15Z","timestamp":1485980355000},"page":"1942-1945","source":"Crossref","is-referenced-by-count":6,"title":["A <i>vanG<\/i>-type locus in <i>Clostridium argentinense<\/i>"],"prefix":"10.1093","volume":"70","author":[{"given":"Nicolas","family":"Berthet","sequence":"first","affiliation":[{"name":"1 \u00a0Centre National de la Recherche Scientifique, UMR3569 Paris, France"},{"name":"2 \u00a0Centre International de la Recherche M\u00e9dicale de Franceville, Unit\u00e9 des Zoonoses et Maladies Emergentes, Franceville, Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"P\u00e9richon","sequence":"additional","affiliation":[{"name":"3 \u00a0Institut Pasteur, Unit\u00e9 des Agents 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