{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T09:30:10Z","timestamp":1740130210908,"version":"3.37.3"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"8","funder":[{"name":"Jiangsu Provincial Bureau of Health","award":["XK201150"],"award-info":[{"award-number":["XK201150"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["81302480"],"award-info":[{"award-number":["81302480"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Foundation of Nanjing Medical Science and Technique Development","award":["QRX11106"],"award-info":[{"award-number":["QRX11106"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The microscopic observation drug susceptibility (MODS) assay has been used for Mycobacterium tuberculosis detection and anti-TB drug susceptibility tests for several years, and our study aimed to evaluate the accuracy of MODS in detecting pyrazinamide resistance in MDR TB suspects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One hundred and forty-eight clinical isolates were collected from 148 MDR TB suspects in the Nanjing Chest Hospital, and the MODS and the mycobacteria growth indicator tube (MGIT) 960 methods for pyrazinamide susceptibility testing were conducted for each isolate independently. pncA gene sequencing was applied to confirm results showing discrepancy between the MODS and MGIT 960 methods. The McNemar \u03c72 test was employed to evaluate the paired 2\u200a\u00d7\u200a2 table.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the MGIT 960 method, the sensitivity and specificity of the MODS assay were 95.5% and 93.3%, respectively, with a high accuracy of 94.5%. The \u03ba value of 0.89 showed near-perfect agreement for the two methods in determining pyrazinamide susceptibility. Eight clinical isolates showed inconsistent results by MODS and MGIT 960. After sequencing the pncA gene of the eight isolates, MODS and MGIT 960 showed no significant difference in detecting pyrazinamide resistance when compared with pncA gene sequencing (P\u200a=\u200a0.655 for MODS and P\u200a=\u200a0.564 for MGIT 960).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The high accuracy of MODS in detecting pyrazinamide resistance showed that the MODS method would be an alternative for pyrazinamide susceptibility testing; other mutations affecting resistance to pyrazinamide need to be identified in order to elucidate the discrepant results between phenotype- and genotype-based methods.<\/jats:p><\/jats:sec>","DOI":"10.1093\/jac\/dkv112","type":"journal-article","created":{"date-parts":[[2015,5,8]],"date-time":"2015-05-08T01:21:42Z","timestamp":1431048102000},"page":"2295-2299","source":"Crossref","is-referenced-by-count":4,"title":["The indirect microscopic observation drug susceptibility assay demonstrated high concordance with the indirect MGIT method for pyrazinamide susceptibility testing"],"prefix":"10.1093","volume":"70","author":[{"given":"Xiaoyuan","family":"Wu","sequence":"first","affiliation":[{"name":"1 \u00a0Central Laboratory, Nanjing Chest Hospital, Medical School of Southeast University, Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Lu","sequence":"additional","affiliation":[{"name":"2 \u00a0Department of Chronic Communicable Disease, Center for Disease Control and Prevention of Jiangsu Province, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Shao","sequence":"additional","affiliation":[{"name":"2 \u00a0Department of Chronic Communicable Disease, Center for Disease Control and Prevention of Jiangsu Province, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiao","family":"Liu","sequence":"additional","affiliation":[{"name":"2 \u00a0Department of Chronic Communicable Disease, Center for Disease Control and Prevention of Jiangsu Province, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xudong","family":"Shi","sequence":"additional","affiliation":[{"name":"3 \u00a0Clinical Laboratory, Nanjing Chest Hospital, Medical School of Southeast University, Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangdong","family":"Wang","sequence":"additional","affiliation":[{"name":"3 \u00a0Clinical Laboratory, Nanjing Chest Hospital, Medical School of Southeast University, Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunmei","family":"Li","sequence":"additional","affiliation":[{"name":"3 \u00a0Clinical Laboratory, Nanjing Chest Hospital, Medical School of Southeast University, Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Limei","family":"Zhu","sequence":"additional","affiliation":[{"name":"2 \u00a0Department of Chronic Communicable Disease, Center for Disease Control and Prevention of Jiangsu Province, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"2 \u00a0Department of Chronic Communicable Disease, Center for Disease Control and Prevention of Jiangsu Province, Nanjing, China"},{"name":"4 \u00a0School of Public Health, Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2015,5,7]]},"reference":[{"author":"WHO","article-title":"Global Tuberculosis Report 2013","key":"2022020121031074900_DKV112C1"},{"key":"2022020121031074900_DKV112C2","doi-asserted-by":"crossref","first-page":"5465","DOI":"10.1128\/AAC.01300-12","article-title":"Pyrazinamide may improve fluoroquinolone-based treatment of multidrug-resistant tuberculosis","volume":"56","author":"Chang","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"2022020121031074900_DKV112C3","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1016\/S0140-6736(12)61080-0","article-title":"14-day bactericidal activity of PA-824, bedaquiline, pyrazinamide, and moxifloxacin combinations: a randomised trial","volume":"380","author":"Diacon","year":"2012","journal-title":"Lancet"},{"key":"2022020121031074900_DKV112C4","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.tube.2013.10.006","article-title":"Pyrazinamide susceptibility testing of Mycobacterium tuberculosis by high resolution melt analysis","volume":"94","author":"Pholwat","year":"2014","journal-title":"Tuberculosis"},{"key":"2022020121031074900_DKV112C5","doi-asserted-by":"crossref","first-page":"3726","DOI":"10.1128\/JCM.00620-12","article-title":"Gene sequencing for routine verification of pyrazinamide resistance in Mycobacterium tuberculosis: a role for pncA but not rpsA","volume":"50","author":"Alexander","year":"2012","journal-title":"J Clin Microbiol"},{"key":"2022020121031074900_DKV112C6","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1093\/jac\/dku384","article-title":"Evaluation of the microscopic observation drug susceptibility assay for the rapid detection of MDR-TB and XDR-TB in China: a prospective multicentre study","volume":"70","author":"Huang","year":"2015","journal-title":"J Antimicrob Chemother"},{"key":"2022020121031074900_DKV112C7","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1183\/09031936.00059812","article-title":"Second-line anti-tuberculosis drug concentrations for susceptibility testing in the MODS assay","volume":"41","author":"Fitzwater","year":"2013","journal-title":"Eur Respir J"},{"volume-title":"Guidelines for Surveillance of Drug Resistance in 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mycobacteria using microscopic observation of drug susceptibility (MODS) methodology","volume":"61","author":"Agarwal","year":"2014","journal-title":"Indian J Tuberc"},{"key":"2022020121031074900_DKV112C12","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1056\/NEJMoa055524","article-title":"Microscopic-observation drug-susceptibility assay for the diagnosis of TB","volume":"355","author":"Moore","year":"2006","journal-title":"N Engl J Med"},{"key":"2022020121031074900_DKV112C13","doi-asserted-by":"crossref","first-page":"227","DOI":"10.5588\/ijtld.13.0229","article-title":"Second-line drug susceptibility breakpoints for Mycobacterium tuberculosis using the MODS assay","volume":"18","author":"Trollip","year":"2014","journal-title":"Int J Tuberc Lung Dis"},{"author":"WHO","article-title":"Guidelines for the Programmatic Management of Drug-Resistant 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