{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T06:52:47Z","timestamp":1789455167907,"version":"build-2803163510"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,4,1]]},"DOI":"10.1093\/jac\/dkx475","type":"journal-article","created":{"date-parts":[[2017,11,20]],"date-time":"2017-11-20T12:08:44Z","timestamp":1511179724000},"page":"1045-1053","source":"Crossref","is-referenced-by-count":12,"title":["Dolutegravir reshapes the genetic diversity of HIV-1 reservoirs"],"prefix":"10.1093","volume":"73","author":[{"given":"Pierre","family":"Gantner","sequence":"first","affiliation":[{"name":"Virology Laboratory, Strasbourg University Hospitals, Strasbourg, France"},{"name":"Strasbourg University, INSERM, UMR-S 1109, F-67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guinevere Q","family":"Lee","sequence":"additional","affiliation":[{"name":"The Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Rey","sequence":"additional","affiliation":[{"name":"Le Trait d\u2019Union, HIV-infection care center, CHU de Strasbourg, Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibault","family":"Mesplede","sequence":"additional","affiliation":[{"name":"McGill University AIDS Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec, Canada"},{"name":"Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marialuisa","family":"Partisani","sequence":"additional","affiliation":[{"name":"Le Trait d\u2019Union, HIV-infection care center, CHU de Strasbourg, Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Cheneau","sequence":"additional","affiliation":[{"name":"Le Trait d\u2019Union, HIV-infection care center, CHU de Strasbourg, Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevi\u00e8ve","family":"Beck-Wirth","sequence":"additional","affiliation":[{"name":"Internal Medicine Department, HIV-infection care center, GHR Mulhouse Sud Alsace, Mulhouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Faller","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, H\u00f4pital Nord Franche Comt\u00e9, Belfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahsa","family":"Mohseni-Zadeh","sequence":"additional","affiliation":[{"name":"Internal Medicine and Rheumatology Department, H\u00f4pital Civil de Colmar, Colmar, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Martinot","sequence":"additional","affiliation":[{"name":"Internal Medicine and Rheumatology Department, H\u00f4pital Civil de Colmar, Colmar, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A","family":"Wainberg","sequence":"additional","affiliation":[{"name":"McGill University AIDS Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec, Canada"},{"name":"Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira","family":"Fafi-Kremer","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Strasbourg University Hospitals, Strasbourg, France"},{"name":"Strasbourg University, INSERM, UMR-S 1109, F-67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,12,13]]},"reference":[{"key":"key\n\t\t\t\t20180316035332_dkx475-B1","doi-asserted-by":"crossref","first-page":"8869","DOI":"10.1073\/pnas.95.15.8869","article-title":"Early establishment of a pool of latently infected, resting CD4+ T cells during primary HIV-1 infection","volume":"95","author":"Chun","year":"1998","journal-title":"Proc Natl Acad Sci USA"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B2","doi-asserted-by":"crossref","first-page":"4819","DOI":"10.1073\/pnas.0736332100","article-title":"Heterogeneous clearance rates of long-lived lymphocytes infected with HIV: intrinsic stability predicts lifelong persistence","volume":"100","author":"Strain","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B3","doi-asserted-by":"crossref","first-page":"11.","DOI":"10.1186\/1742-4690-10-11","article-title":"Cellular and molecular mechanisms involved in the establishment of HIV-1 latency","volume":"10","author":"Donahue","year":"2013","journal-title":"Retrovirology"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B4","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/nm880","article-title":"Long-term follow-up studies confirm the stability of the latent reservoir for HIV-1 in resting CD4+ T cells","volume":"9","author":"Siliciano","year":"2003","journal-title":"Nat Med"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B5","doi-asserted-by":"crossref","first-page":"e463","DOI":"10.1016\/S2352-3018(16)30055-8","article-title":"Combined effect of Vacc-4x, recombinant human granulocyte macrophage colony-stimulating factor vaccination, and romidepsin on the HIV-1 reservoir (REDUC): a single-arm, phase 1B\/2A trial","volume":"3","author":"Leth","year":"2016","journal-title":"Lancet HIV"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B6","doi-asserted-by":"crossref","first-page":"e1003211.","DOI":"10.1371\/journal.ppat.1003211","article-title":"Post-treatment HIV-1 controllers with a long-term virological remission after the interruption of early initiated antiretroviral therapy ANRS VISCONTI Study","volume":"9","author":"S\u00e1ez-Ciri\u00f3n","year":"2013","journal-title":"PLoS Pathog"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B7","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1086\/649569","article-title":"No evidence for decay of the latent reservoir in HIV-1-infected patients receiving intensive enfuvirtide-containing antiretroviral therapy","volume":"201","author":"Gandhi","year":"2010","journal-title":"J Infect Dis"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B8","doi-asserted-by":"crossref","first-page":"e18889","DOI":"10.7554\/eLife.18889","article-title":"Establishment and stability of the latent HIV-1 DNA reservoir","volume":"5","author":"Brodin","year":"2016","journal-title":"Elife"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B9","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1097\/QAD.0b013e32833032d4","article-title":"Factors influencing peripheral blood mononuclear cell-associated HIV-1 DNA level after long-term suppressive antiretroviral therapy in 236 patients","volume":"23","author":"Burgard","year":"2009","journal-title":"AIDS"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B10","doi-asserted-by":"crossref","first-page":"E4987","DOI":"10.1073\/pnas.1308313110","article-title":"The HIV-1 reservoir in eight patients on long-term suppressive antiretroviral therapy is stable with few genetic changes over time","volume":"110","author":"Josefsson","year":"2013","journal-title":"Proc Natl Acad Sci USA"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B11","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1093\/infdis\/jiv092","article-title":"Longitudinal genetic characterization reveals that cell proliferation maintains a persistent HIV type 1 DNA pool during effective HIV therapy","volume":"212","author":"von Stockenstrom","year":"2015","journal-title":"J Infect Dis"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B12","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1093\/jac\/dkv084","article-title":"Combined ART started during acute HIV infection protects central memory CD4+ T cells and can induce remission","volume":"70","author":"Ch\u00e9ret","year":"2015","journal-title":"J Antimicrob Chemother"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B13","volume-title":"Abstracts of the Conference on Retroviruses and Opportunistic Infections, Seattle, WA, USA","author":"Katusiime","year":"2017"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/nature16933","article-title":"Persistent HIV-1 replication maintains the tissue reservoir during therapy","volume":"530","author":"Lorenzo-Redondo","year":"2016","journal-title":"Nature"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B15","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1093\/jac\/dkv395","article-title":"Drug resistance and tropism as markers of the dynamics of HIV-1 DNA quasispecies in blood cells of heavily pretreated patients who achieved sustained virological suppression","volume":"71","author":"Gantner","year":"2016","journal-title":"J Antimicrob Chemother"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B16","doi-asserted-by":"crossref","first-page":"2689","DOI":"10.1172\/JCI93289","article-title":"Clonal expansion of genome-intact HIV-1 in functionally polarized Th1 CD4+ T cells","volume":"127","author":"Lee","year":"2017","journal-title":"J Clin Invest"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B17","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.cell.2015.01.020","article-title":"HIV-1 integration landscape during latent and active infection","volume":"160","author":"Cohn","year":"2015","journal-title":"Cell"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B18","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1093\/cid\/ciu221","article-title":"Dolutegravir: a next-generation integrase inhibitor for treatment of HIV infection","volume":"59","author":"Osterholzer","year":"2014","journal-title":"Clin Infect Dis"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B19","doi-asserted-by":"crossref","first-page":"3703","DOI":"10.3390\/v7072790","article-title":"Resistance against integrase strand transfer inhibitors and relevance to HIV persistence","volume":"7","author":"Mespl\u00e8de","year":"2015","journal-title":"Viruses"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B20","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1139\/cjm-2015-0725","article-title":"Might dolutegravir be part of a functional cure for HIV?","volume":"62","author":"Wainberg","year":"2016","journal-title":"Can J Microbiol"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.virusres.2016.07.006","article-title":"Clinical benefit of dolutegravir in HIV-1 management related to the high genetic barrier to drug resistance","volume":"239","author":"Brenner","year":"2017","journal-title":"Virus Res"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B22","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1038\/nm.2111","article-title":"HIV-1 replication and immune dynamics are affected by raltegravir intensification of HAART-suppressed subjects","volume":"16","author":"Buz\u00f3n","year":"2010","journal-title":"Nat Med"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B23","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1097\/QAD.0b013e32833d214c","article-title":"Time course of total HIV-1 DNA and 2-long-terminal repeat circles in patients with controlled plasma viremia switching to a raltegravir-containing regimen","volume":"24","author":"Delaugerre","year":"2010","journal-title":"AIDS"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B24","doi-asserted-by":"crossref","first-page":"2809","DOI":"10.1093\/jac\/dku213","article-title":"Impact of raltegravir on HIV-1 RNA and DNA forms following initiation of antiretroviral therapy in treatment-naive patients","volume":"69","author":"Stephan","year":"2014","journal-title":"J Antimicrob Chemother"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B25","doi-asserted-by":"crossref","first-page":"431","DOI":"10.2174\/1570162X14666160324125536","article-title":"HIV-1 latency and eradication: past, present and future","volume":"14","author":"Datta","year":"2016","journal-title":"Curr HIV Res"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B26","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/jmv.21390","article-title":"LTR real-time PCR for HIV-1 DNA quantitation in blood cells for early diagnosis in infants born to seropositive mothers treated in HAART area (ANRS CO 01)","volume":"81","author":"Avettand-F\u00e8no\u00ebl","year":"2009","journal-title":"J Med Virol"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B27","doi-asserted-by":"crossref","first-page":"i304","DOI":"10.1093\/bioinformatics\/btr251","article-title":"Systematic exploration of error sources in pyrosequencing flowgram data","volume":"27","author":"Balzer","year":"2011","journal-title":"Bioinformatics"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B28","doi-asserted-by":"crossref","first-page":"e1002529","DOI":"10.1371\/journal.ppat.1002529","article-title":"Whole genome deep sequencing of HIV-1 reveals the impact of early minor variants upon immune recognition during acute infection","volume":"8","author":"Henn","year":"2012","journal-title":"PLoS Pathog"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B29","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/nature01707","article-title":"The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA","volume":"424","author":"Zhang","year":"2003","journal-title":"Nature"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B30","doi-asserted-by":"crossref","first-page":"e102857.","DOI":"10.1371\/journal.pone.0102857","article-title":"HIV-1 tropism dynamics and phylogenetic analysis from longitudinal ultra-deep sequencing data of CCR5- and CXCR4-using variants","volume":"9","author":"Sede","year":"2014","journal-title":"PLoS One"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B31","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1080\/13854046.2017.1306111","article-title":"Review of doing Bayesian data analysis: a tutorial with R, JAGS, and Stan (second edition)","volume":"31","author":"Gaudet","year":"2017","journal-title":"Clin Neuropsychol"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B32","doi-asserted-by":"crossref","first-page":"e1006510.","DOI":"10.1371\/journal.ppat.1006510","article-title":"Rare HIV-1 transmitted\/founder lineages identified by deep viral sequencing contribute to rapid shifts in dominant quasispecies during acute and early infection","volume":"13","author":"Kijak","year":"2017","journal-title":"PLoS Pathog"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B33","doi-asserted-by":"crossref","first-page":"e64219.","DOI":"10.1371\/journal.pone.0064219","article-title":"A single HIV-1 cluster and a skewed immune homeostasis drive the early spread of HIV among resting CD4+ cell subsets within one month post-infection","volume":"8","author":"Bacchus","year":"2013","journal-title":"PLoS One"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B34","doi-asserted-by":"crossref","first-page":"3098","DOI":"10.1128\/JVI.70.5.3098-3107.1996","article-title":"Genetic characterization of human immunodeficiency virus type 1 in blood and genital secretions: evidence for viral compartmentalization and selection during sexual transmission","volume":"70","author":"Zhu","year":"1996","journal-title":"J Virol"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B35","doi-asserted-by":"crossref","first-page":"9523","DOI":"10.1073\/pnas.1120248109","article-title":"Immediate antiviral therapy appears to restrict resting CD4+ cell HIV-1 infection without accelerating the decay of latent infection","volume":"109","author":"Archin","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B36","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/nri2674","article-title":"The immune response during acute HIV-1 infection: clues for vaccine development","volume":"10","author":"McMichael","year":"2010","journal-title":"Nat Rev Immunol"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B37","doi-asserted-by":"crossref","first-page":"7131","DOI":"10.4049\/jimmunol.0803658","article-title":"Kinetics of expansion of epitope-specific T cell responses during primary HIV-1 infection","volume":"182","author":"Turnbull","year":"2009","journal-title":"J Immunol"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B38","doi-asserted-by":"crossref","first-page":"3879","DOI":"10.1073\/pnas.0800050105","article-title":"Low-level viremia persists for at least 7 years in patients on suppressive antiretroviral therapy","volume":"105","author":"Palmer","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B39","doi-asserted-by":"crossref","first-page":"3487","DOI":"10.3390\/v6093487","article-title":"Fitness impaired drug resistant HIV-1 is not compromised in cell-to-cell transmission or establishment of and reactivation from latency","volume":"6","author":"Bastarache","year":"2014","journal-title":"Viruses"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B40","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1038\/nm.1972","article-title":"HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation","volume":"15","author":"Chomont","year":"2009","journal-title":"Nat Med"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B41","doi-asserted-by":"crossref","first-page":"e1004955.","DOI":"10.1371\/journal.ppat.1004955","article-title":"HIV latency is established directly and early in both resting and activated primary CD4 T cells","volume":"11","author":"Chavez","year":"2015","journal-title":"PLoS Pathog"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B42","doi-asserted-by":"crossref","first-page":"e1001053.","DOI":"10.1371\/journal.ppat.1001053","article-title":"HIV-1 populations in semen arise through multiple mechanisms","volume":"6","author":"Anderson","year":"2010","journal-title":"PLoS Pathog"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B43","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.1073\/pnas.1318249111","article-title":"Persistent HIV-1 replication is associated with lower antiretroviral drug concentrations in lymphatic tissues","volume":"111","author":"Fletcher","year":"2014","journal-title":"Proc Natl Acad Sci USA"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B44","doi-asserted-by":"crossref","first-page":"2696","DOI":"10.1128\/JVI.06591-11","article-title":"Characterization of the R263K mutation in HIV-1 integrase that confers low-level resistance to the second-generation integrase strand transfer inhibitor dolutegravir","volume":"86","author":"Quashie","year":"2012","journal-title":"J Virol"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B45","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1016\/S0140-6736(13)61221-0","article-title":"Dolutegravir versus raltegravir in antiretroviral-experienced, integrase-inhibitor-naive adults with HIV: week 48 results from the randomised, double-blind, non-inferiority SAILING study","volume":"382","author":"Cahn","year":"2013","journal-title":"Lancet"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B46","doi-asserted-by":"crossref","first-page":"7.","DOI":"10.1186\/1742-4690-11-7","article-title":"The M50I polymorphic substitution in association with the R263K mutation in HIV-1 subtype B integrase increases drug resistance but does not restore viral replicative fitness","volume":"11","author":"Wares","year":"2014","journal-title":"Retrovirology"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B47","doi-asserted-by":"crossref","first-page":"22.","DOI":"10.1186\/1742-4690-10-22","article-title":"Viral fitness cost prevents HIV-1 from evading dolutegravir drug pressure","volume":"10","author":"Mespl\u00e8de","year":"2013","journal-title":"Retrovirology"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B48","doi-asserted-by":"crossref","first-page":"e00157-17.","DOI":"10.1128\/mBio.00157-17","article-title":"The R263K dolutegravir resistance-associated substitution progressively decreases HIV-1 integration","volume":"8","author":"Mespl\u00e8de","year":"2017","journal-title":"MBio"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B49","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1002\/psp4.12200","article-title":"A mathematical model to predict HIV virological failure and elucidate the role of lymph node drug penetration","volume":"6","author":"Sanche","year":"2017","journal-title":"CPT Pharmacometrics Syst Pharmacol"},{"key":"key\n\t\t\t\t20180316035332_dkx475-B50","doi-asserted-by":"crossref","first-page":"7086","DOI":"10.1128\/AAC.01474-16","article-title":"Antiviral activity of bictegravir (GS-9883), a novel potent HIV-1 integrase strand transfer inhibitor with an improved resistance profile","volume":"60","author":"Tsiang","year":"2016","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jac\/article-pdf\/73\/4\/1045\/24269473\/dkx475.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T18:07:35Z","timestamp":1659895655000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/73\/4\/1045\/4735325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,13]]},"references-count":50,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2017,12,13]]},"published-print":{"date-parts":[[2018,4,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkx475","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2018,4]]},"published":{"date-parts":[[2017,12,13]]}}}