{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:35:23Z","timestamp":1789990523330,"version":"4.0.1"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T00:00:00Z","timestamp":1519776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006477","name":"National Taiwan University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006477","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005762","name":"National Taiwan University Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,6,1]]},"DOI":"10.1093\/jac\/dky054","type":"journal-article","created":{"date-parts":[[2018,2,13]],"date-time":"2018-02-13T07:08:06Z","timestamp":1518505686000},"page":"1509-1516","source":"Crossref","is-referenced-by-count":57,"title":["A putative RND-type efflux pump, H239_3064, contributes to colistin resistance through CrrB in Klebsiella pneumoniae"],"prefix":"10.1093","volume":"73","author":[{"given":"Yi-Hsiang","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu-Lung","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Tsung","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan"},{"name":"Institute of Emergency and Critical Care Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Town","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan"},{"name":"Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2018,2,28]]},"reference":[{"key":"key\n\t\t\t\t20180626060844_dky054-B1","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/S1473-3099(13)70190-7","article-title":"Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases","volume":"13","author":"Munoz-Price","year":"2013","journal-title":"Lancet Infect Dis"},{"key":"key\n\t\t\t\t20180626060844_dky054-B2","doi-asserted-by":"crossref","first-page":"e69428","DOI":"10.1371\/journal.pone.0069428","article-title":"National surveillance study on carbapenem non-susceptible Klebsiella pneumoniae in Taiwan: the emergence and rapid dissemination of KPC-2 carbapenemase","volume":"8","author":"Chiu","year":"2013","journal-title":"PLoS One"},{"key":"key\n\t\t\t\t20180626060844_dky054-B3","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1586\/eri.12.78","article-title":"Colistin: an update on the antibiotic of the 21st century","volume":"10","author":"Biswas","year":"2012","journal-title":"Expert Rev Anti Infect Ther"},{"key":"key\n\t\t\t\t20180626060844_dky054-B4","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.ijantimicag.2004.10.001","article-title":"Evaluation of colistin as an agent against multi-resistant Gram-negative bacteria","volume":"25","author":"Li","year":"2005","journal-title":"Int J Antimicrob Agents"},{"key":"key\n\t\t\t\t20180626060844_dky054-B5","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1185\/03007995.2015.1018989","article-title":"Colistin, mechanisms and prevalence of resistance","volume":"31","author":"Bialvaei","year":"2015","journal-title":"Curr Med Res Opin"},{"key":"key\n\t\t\t\t20180626060844_dky054-B6","doi-asserted-by":"crossref","first-page":"36077","DOI":"10.1074\/jbc.M706172200","article-title":"An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli","volume":"282","author":"Yan","year":"2007","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20180626060844_dky054-B7","doi-asserted-by":"crossref","first-page":"4124","DOI":"10.1128\/JB.186.13.4124-4133.2004","article-title":"The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid A and polymyxin resistance in Salmonella enterica","volume":"186","author":"Lee","year":"2004","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B8","doi-asserted-by":"crossref","first-page":"643.","DOI":"10.3389\/fmicb.2014.00643","article-title":"Mechanisms of polymyxin resistance: acquired and intrinsic resistance in bacteria","volume":"5","author":"Olaitan","year":"2014","journal-title":"Front Microbiol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B9","doi-asserted-by":"crossref","first-page":"60.","DOI":"10.1186\/1423-0127-17-60","article-title":"Molecular characterization of the PhoPQ-PmrD-PmrAB mediated pathway regulating polymyxin B resistance in Klebsiella pneumoniae CG43","volume":"17","author":"Cheng","year":"2010","journal-title":"J Biomed Sci"},{"key":"key\n\t\t\t\t20180626060844_dky054-B10","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1146\/annurev-micro-092412-155751","article-title":"The biology of the PmrA\/PmrB two-component system: the major regulator of lipopolysaccharide modifications","volume":"67","author":"Chen","year":"2013","journal-title":"Annu Rev Microbiol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B11","doi-asserted-by":"crossref","first-page":"5521","DOI":"10.1128\/AAC.01480-13","article-title":"In vivo emergence of colistin resistance in Klebsiella pneumoniae producing KPC-type carbapenemases mediated by insertional inactivation of the PhoQ\/PhoP mgrB regulator","volume":"57","author":"Cannatelli","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20180626060844_dky054-B12","doi-asserted-by":"crossref","first-page":"5696","DOI":"10.1128\/AAC.03110-14","article-title":"MgrB inactivation is a common mechanism of colistin resistance in KPC-producing Klebsiella pneumoniae of clinical origin","volume":"58","author":"Cannatelli","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20180626060844_dky054-B13","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/j.ijantimicag.2014.07.020","article-title":"Worldwide emergence of colistin resistance in Klebsiella pneumoniae from healthy humans and patients in Lao PDR, Thailand, Israel, Nigeria and France owing to inactivation of the PhoP\/PhoQ regulator mgrB: an epidemiological and molecular study","volume":"44","author":"Olaitan","year":"2014","journal-title":"Int J Antimicrob Agents"},{"key":"key\n\t\t\t\t20180626060844_dky054-B14","doi-asserted-by":"crossref","first-page":"5761","DOI":"10.1128\/AAC.05391-11","article-title":"PhoQ mutations promote lipid A modification and polymyxin resistance of Pseudomonas aeruginosa found in colistin-treated cystic fibrosis patients","volume":"55","author":"Miller","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20180626060844_dky054-B15","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1128\/AAC.04037-14","article-title":"Genomic and transcriptomic analyses of colistin-resistant clinical isolates of Klebsiella pneumoniae reveal multiple pathways of resistance","volume":"59","author":"Wright","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20180626060844_dky054-B16","doi-asserted-by":"crossref","first-page":"3709","DOI":"10.1128\/AAC.00009-16","article-title":"Amino acid substitutions of CrrB responsible for resistance to colistin through CrrC in Klebsiella pneumoniae","volume":"60","author":"Cheng","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20180626060844_dky054-B17","doi-asserted-by":"crossref","first-page":"e01423-17","DOI":"10.1128\/AAC.01423-17","article-title":"High-level resistance to colistin mediated by various mutations in the crrB gene among carbapenemase-producing Klebsiella pneumoniae","volume":"61","author":"Jayol","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20180626060844_dky054-B18","doi-asserted-by":"crossref","first-page":"2909","DOI":"10.1128\/AAC.04763-14","article-title":"Colistin resistance mechanisms in Klebsiella pneumoniae strains from Taiwan","volume":"59","author":"Cheng","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20180626060844_dky054-B19","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1084\/jem.20030857","article-title":"novel virulence gene in Klebsiella pneumoniae strains causing primary liver abscess and septic metastatic complications","volume":"199","author":"Fang","year":"2004","journal-title":"J Exp Med"},{"key":"key\n\t\t\t\t20180626060844_dky054-B20","doi-asserted-by":"crossref","first-page":"6557","DOI":"10.1128\/jb.172.11.6557-6567.1990","article-title":"Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria","volume":"172","author":"Herrero","year":"1990","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B21","doi-asserted-by":"crossref","first-page":"7926","DOI":"10.1128\/JB.186.23.7926-7935.2004","article-title":"Global transposon mutagenesis and essential gene analysis of Helicobacter pylori","volume":"186","author":"Salama","year":"2004","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B22","doi-asserted-by":"crossref","first-page":"e00306-15","DOI":"10.1128\/mBio.00306-15","article-title":"Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons","volume":"6","author":"Wetmore","year":"2015","journal-title":"MBio"},{"key":"key\n\t\t\t\t20180626060844_dky054-B23","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"key\n\t\t\t\t20180626060844_dky054-B24","doi-asserted-by":"crossref","first-page":"6228","DOI":"10.1128\/jb.179.20.6228-6237.1997","article-title":"Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization","volume":"179","author":"Link","year":"1997","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B25","doi-asserted-by":"crossref","first-page":"e46783","DOI":"10.1371\/journal.pone.0046783","article-title":"Amino acid substitutions of MagA in Klebsiella pneumoniae affect the biosynthesis of the capsular polysaccharide","volume":"7","author":"Lin","year":"2012","journal-title":"PLoS One"},{"key":"key\n\t\t\t\t20180626060844_dky054-B26","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1093\/jac\/dkt481","article-title":"Redundancy in the periplasmic adaptor proteins AcrA and AcrE provides resilience and an ability to export substrates of multidrug efflux","volume":"69","author":"Smith","year":"2014","journal-title":"J Antimicrob Chemother"},{"key":"key\n\t\t\t\t20180626060844_dky054-B27","doi-asserted-by":"crossref","first-page":"e96288","DOI":"10.1371\/journal.pone.0096288","article-title":"Role of novel multidrug efflux pump involved in drug resistance in Klebsiella pneumoniae","volume":"9","author":"Srinivasan","year":"2014","journal-title":"PLoS One"},{"key":"key\n\t\t\t\t20180626060844_dky054-B28","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.rvsc.2014.05.013","article-title":"Effect of the inhibitors phenylalanine arginyl \u03b2-naphthylamide (PA\u03b2N) and 1-(1-naphthylmethyl)-piperazine (NMP) on expression of genes in multidrug efflux systems of Escherichia coli isolates from bovine mastitis","volume":"97","author":"Barrero","year":"2014","journal-title":"Res Vet Sci"},{"key":"key\n\t\t\t\t20180626060844_dky054-B29","doi-asserted-by":"crossref","first-page":"6804","DOI":"10.1128\/JB.187.19.6804-6815.2005","article-title":"A periplasmic drug-binding site of the AcrB multidrug efflux pump: a crystallographic and site-directed mutagenesis study","volume":"187","author":"Yu","year":"2005","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B30","doi-asserted-by":"crossref","first-page":"228","DOI":"10.3389\/fmicb.2017.00228","article-title":"Carbonyl cyanide m-chlorophenylhydrazine (CCCP) reverses resistance to colistin, but not to carbapenems and tigecycline in multidrug-resistant Enterobacteriaceae","volume":"8","author":"Osei Sekyere","year":"2017","journal-title":"Front Microbiol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B31","doi-asserted-by":"crossref","first-page":"3215","DOI":"10.1128\/AAC.00248-16","article-title":"Effects of efflux pump inhibitors on colistin resistance in multidrug-resistant Gram-negative bacteria","volume":"60","author":"Ni","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"key\n\t\t\t\t20180626060844_dky054-B32","doi-asserted-by":"crossref","first-page":"42483","DOI":"10.1038\/srep42483","article-title":"The secondary resistome of multidrug-resistant Klebsiella pneumoniae","volume":"7","author":"Jana","year":"2017","journal-title":"Sci Rep"},{"key":"key\n\t\t\t\t20180626060844_dky054-B33","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1128\/jb.178.4.1047-1052.1996","article-title":"Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase","volume":"178","author":"Nassau","year":"1996","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B34","doi-asserted-by":"crossref","first-page":"19668","DOI":"10.1074\/jbc.M110.122598","article-title":"A membrane-located glycosyltransferase complex required for biosynthesis of the d-galactan I lipopolysaccharide O antigen in Klebsiella pneumoniae","volume":"285","author":"Kos","year":"2010","journal-title":"J Biol Chem"},{"key":"key\n\t\t\t\t20180626060844_dky054-B35","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1128\/JB.185.5.1634-1641.2003","article-title":"Synthesis of a Klebsiella pneumoniae O-antigen heteropolysaccharide (O12) requires an ABC 2 transporter","volume":"185","author":"Izquierdo","year":"2003","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B36","first-page":"383","article-title":"Occurrence of selected genes of the Klebsiella pneumoniae clusters waa and wb for lipolysaccharide synthesis in reference and epidemic strains","volume":"57","author":"Gierczynski","year":"2005","journal-title":"Med Dosw Mikrobiol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B37","doi-asserted-by":"crossref","first-page":"4764","DOI":"10.1128\/JB.182.17.4764-4772.2000","article-title":"Mutations in each of the tol genes of Pseudomonas putida reveal that they are critical for maintenance of outer membrane stability","volume":"182","author":"Llamas","year":"2000","journal-title":"J Bacteriol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B38","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/0022-2836(89)90330-6","article-title":"Genetic analysis of the switch that controls porin gene expression in Escherichia coli K-12","volume":"210","author":"Slauch","year":"1989","journal-title":"J Mol Biol"},{"key":"key\n\t\t\t\t20180626060844_dky054-B39","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1093\/jac\/dkq431","article-title":"ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China","volume":"66","author":"Qi","year":"2011","journal-title":"J Antimicrob Chemother"},{"key":"key\n\t\t\t\t20180626060844_dky054-B40","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/nature05076","article-title":"Crystal structures of a multidrug transporter reveal a functionally rotating mechanism","volume":"443","author":"Murakami","year":"2006","journal-title":"Nature"},{"key":"key\n\t\t\t\t20180626060844_dky054-B41","doi-asserted-by":"crossref","first-page":"660.","DOI":"10.3389\/fmicb.2015.00660","article-title":"Interaction of antibacterial compounds with RND efflux pumps in Pseudomonas aeruginosa","volume":"6","author":"Dreier","year":"2015","journal-title":"Front Microbiol"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jac\/article-pdf\/73\/6\/1509\/25088043\/dky054.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T18:04:01Z","timestamp":1570730641000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/73\/6\/1509\/4913718"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,28]]},"references-count":41,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2018,2,28]]},"published-print":{"date-parts":[[2018,6,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dky054","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2018,6]]},"published":{"date-parts":[[2018,2,28]]}}}