{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T13:23:32Z","timestamp":1793021012928,"version":"4.5.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2019,3,30]],"date-time":"2019-03-30T00:00:00Z","timestamp":1553904000000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Hong Kong Research Grant Council Collaborative Research Fund","award":["C5026-16G"],"award-info":[{"award-number":["C5026-16G"]}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872523"],"award-info":[{"award-number":["31872523"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,1]]},"DOI":"10.1093\/jac\/dkz117","type":"journal-article","created":{"date-parts":[[2019,3,14]],"date-time":"2019-03-14T16:11:12Z","timestamp":1552579872000},"page":"1807-1811","source":"Crossref","is-referenced-by-count":17,"title":["Characterization of the stability and dynamics of Tn6330 in an Escherichia coli strain by nanopore long reads"],"prefix":"10.1093","volume":"74","author":[{"given":"Ruichao","family":"Li","sequence":"first","affiliation":[{"name":"Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, PR China"},{"name":"The State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China"},{"name":"Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaichao","family":"Chen","sequence":"additional","affiliation":[{"name":"Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, PR China"},{"name":"The State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward Wai-Chi","family":"Chan","sequence":"additional","affiliation":[{"name":"Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, PR China"},{"name":"The State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, PR China"},{"name":"The State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,3,29]]},"reference":[{"key":"2019062107401546400_dkz117-B1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1473-3099(15)00424-7","article-title":"Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study","volume":"16","author":"Liu","year":"2015","journal-title":"Lancet Infect Dis"},{"key":"2019062107401546400_dkz117-B2","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.ijantimicag.2016.06.023","article-title":"Molecular mechanisms of polymyxin resistance: knowns and unknowns","volume":"48","author":"Baron","year":"2016","journal-title":"Int J Antimicrob Agents"},{"key":"2019062107401546400_dkz117-B3","doi-asserted-by":"crossref","first-page":"a025288","DOI":"10.1101\/cshperspect.a025288","article-title":"Polymyxin: alternative mechanisms of action and resistance","volume":"6","author":"Trimble","year":"2016","journal-title":"Cold Spring Harb Perspect Med"},{"key":"2019062107401546400_dkz117-B4","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2016.21.27.30280","article-title":"Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016","volume":"21","author":"Xavier","year":"2016","journal-title":"Euro Surveill"},{"key":"2019062107401546400_dkz117-B5","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.3201\/eid2209.160692","article-title":"Chromosomal locations of mcr-1 and blaCTX-M-15 in fluoroquinolone-resistant Escherichia coli ST410","volume":"22","author":"Falgenhauer","year":"2016","journal-title":"Emerg Infect Dis"},{"key":"2019062107401546400_dkz117-B6","doi-asserted-by":"crossref","first-page":"5033","DOI":"10.1128\/AAC.00440-16","article-title":"Detection of the mcr-1 colistin resistance gene in carbapenem-resistant Enterobacteriaceae from different hospitals in China","volume":"60","author":"Yu","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"2019062107401546400_dkz117-B7","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2018.23.6.17-00672","article-title":"Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes","volume":"23","author":"Rebelo","year":"2018","journal-title":"Euro Surveill"},{"key":"2019062107401546400_dkz117-B8","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.ijantimicag.2017.11.015","article-title":"Genetic basis of chromosomally-encoded mcr-1 gene","volume":"51","author":"Li","year":"2018","journal-title":"Int J Antimicrob Agents"},{"key":"2019062107401546400_dkz117-B9","doi-asserted-by":"crossref","first-page":"2094.","DOI":"10.3389\/fmicb.2017.02094","article-title":"Remarkable diversity of Escherichia coli carrying mcr-1 from hospital sewage with the identification of two new mcr-1 variants","volume":"8","author":"Zhao","year":"2017","journal-title":"Front Microbiol"},{"key":"2019062107401546400_dkz117-B10","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1093\/cid\/ciw124","article-title":"Plasmid-mediated colistin-resistant Escherichia coli in bacteremia in Switzerland","volume":"62","author":"Nordmann","year":"2016","journal-title":"Clin Infect Dis"},{"key":"2019062107401546400_dkz117-B11","doi-asserted-by":"crossref","first-page":"6438","DOI":"10.1128\/AAC.01519-16","article-title":"Features of the mcr-1 cassette related to colistin resistance","volume":"60","author":"Zurfluh","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"2019062107401546400_dkz117-B12","first-page":"696","article-title":"Complete genetic analysis of plasmids carrying mcr-1 and other resistance genes in an Escherichia coli isolate of animal origin","volume":"72","author":"Li","year":"2017","journal-title":"J Antimicrob Chemother"},{"key":"2019062107401546400_dkz117-B13","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1093\/jac\/dkw411","article-title":"Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant","volume":"72","author":"Li","year":"2017","journal-title":"J Antimicrob Chemother"},{"key":"2019062107401546400_dkz117-B14","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing\u2014Twenty-Fourth Edition: M100","year":"2014"},{"key":"2019062107401546400_dkz117-B15","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J Comput Biol"},{"key":"2019062107401546400_dkz117-B16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/gigascience\/gix132","article-title":"Efficient generation of complete sequences of MDR-encoding plasmids by rapid assembly of MinION barcoding sequencing data","volume":"7","author":"Li","year":"2018","journal-title":"Gigascience"},{"key":"2019062107401546400_dkz117-B17","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1093\/bioinformatics\/btr039","article-title":"Easyfig: a genome comparison visualizer","volume":"27","author":"Sullivan","year":"2011","journal-title":"Bioinformatics"},{"key":"2019062107401546400_dkz117-B18","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The Sequence Alignment\/Map format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"2019062107401546400_dkz117-B19","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1093\/bib\/bbs012","article-title":"Using Tablet for visual exploration of second-generation sequencing data","volume":"14","author":"Milne","year":"2013","journal-title":"Brief Bioinformatics"},{"key":"2019062107401546400_dkz117-B20","doi-asserted-by":"crossref","first-page":"6973","DOI":"10.1128\/AAC.01457-16","article-title":"A model for transposition of the colistin resistance gene mcr-1 by ISApl1","volume":"60","author":"Snesrud","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"2019062107401546400_dkz117-B21","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.vetmic.2007.10.025","article-title":"ISApl1, a novel insertion element of Actinobacillus pleuropneumoniae, prevents ApxIV-based serological detection of serotype 7 strain AP76","volume":"128","author":"Tegetmeyer","year":"2008","journal-title":"Vet Microbiol"},{"key":"2019062107401546400_dkz117-B22","doi-asserted-by":"crossref","first-page":"5589","DOI":"10.1128\/AAC.00935-16","article-title":"Full-length nucleotide sequences of mcr-1-harboring plasmids isolated from extended-spectrum-\u03b2-lactamase-producing Escherichia coli isolates of different origins","volume":"60","author":"Zurfluh","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"2019062107401546400_dkz117-B23","doi-asserted-by":"crossref","first-page":"e02381-17","DOI":"10.1128\/mBio.02381-17","article-title":"The birth and demise of the ISApl1-mcr-1-ISApl1 composite transposon: the vehicle for transferable colistin resistance","volume":"9","author":"Snesrud","year":"2018","journal-title":"MBio"},{"key":"2019062107401546400_dkz117-B24","doi-asserted-by":"crossref","first-page":"2300","DOI":"10.1093\/jac\/dkw093","article-title":"Detection of the plasmid-mediated mcr-1 gene conferring colistin resistance in human and food isolates of Salmonella enterica and Escherichia coli in England and Wales","volume":"71","author":"Doumith","year":"2016","journal-title":"J Antimicrob Chemother"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jac\/article-pdf\/74\/7\/1807\/28851775\/dkz117.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,21]],"date-time":"2019-09-21T06:37:29Z","timestamp":1569047849000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/74\/7\/1807\/5422994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,29]]},"references-count":24,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2019,3,29]]},"published-print":{"date-parts":[[2019,7,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkz117","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,7]]},"published":{"date-parts":[[2019,3,29]]}}}