{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:29:19Z","timestamp":1792794559451,"version":"4.5.0"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T00:00:00Z","timestamp":1561593600000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Institute of Veterinary Bacteriology"},{"DOI":"10.13039\/100009068","name":"University of Bern","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009068","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Swiss Federal Food Safety and Veterinary Office FSVO","award":["1.18.10"],"award-info":[{"award-number":["1.18.10"]}],"id":[{"id":"https:\/\/ror.org\/01hwpsz06","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Kanchanaphisek Chalermprakiet Endowment Fund","id":[{"id":"https:\/\/ror.org\/0229evh14","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Office of International Affairs and Global Network","id":[{"id":"https:\/\/ror.org\/05ek3m339","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To analyse the genetic context of mecB in two Macrococcus canis strains from dogs, compare the mecB-containing elements with those found in other Macrococcus and Staphylococcus species, and identify possible mobilizable mecB subunits.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Whole genomes of the M. canis strains Epi0076A and KM0218 were sequenced using next-generation sequencing technologies. Multiple PCRs and restriction analysis confirmed structures of mecB-containing elements, circularization and recombination of mecB subunits.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Both M. canis strains contained novel composite pseudo (\u03a8) staphylococcal cassette chromosome mec (SCCmec) elements. Integration site sequences for SCC flanked and subdivided composite \u03a8SCCmecEpi0076A (69569\u2009bp) into \u03a8SCC1Epi0076A-\u03a8SCCmecEpi0076A-\u03a8SCC2Epi0076A and composite \u03a8SCCmecKM0218 (24554\u2009bp) into \u03a8SCCKM0218-\u03a8SCCmecKM0218. Putative \u03b3-haemolysin genes (hlgB and hlgC) were found at the 3\u2032 end of both composite elements. \u03a8SCCmecKM0218 contained a complete mecB gene complex (mecIm-mecR1m-mecB-blaZm) downstream of a new IS21-family member (ISMaca1). \u03a8SCCmecEpi0076A carried a blaZm-deleted mecB gene complex similar to that reported in \u2018Macrococcus goetzii\u2019 CCM4927T. A second mecB gene was found on the 81325\u2009bp MDR plasmid pKM0218 in KM0218. This plasmid contained a complete Tn6045-associated mecB gene complex distinct from that of \u03a8SCCmecKM0218. pKM0218 was almost identical to the mecB-containing plasmid recently reported in Staphylococcus aureus (overall 99.96% nucleotide identity). Mobilization of mecB within an unconventional circularizable structure was observed in Epi0076A as well as chromosomal plasmid insertion via recombination of mecB operons in KM0218.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings provide evidence of both the continuing evolution of mecB-containing elements in macrococci and M. canis as a potential source of the mecB-containing plasmid found in staphylococci.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkz260","type":"journal-article","created":{"date-parts":[[2019,6,6]],"date-time":"2019-06-06T15:12:44Z","timestamp":1559833964000},"page":"2531-2536","source":"Crossref","is-referenced-by-count":24,"title":["Macrococcus canis contains recombinogenic methicillin resistance elements and the mecB plasmid found in Staphylococcus 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