{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T19:51:00Z","timestamp":1769629860873,"version":"3.49.0"},"reference-count":13,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T00:00:00Z","timestamp":1563926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"SpeeDx"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R21AI17256"],"award-info":[{"award-number":["R21AI17256"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R21AI109005"],"award-info":[{"award-number":["R21AI109005"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The emergence of drug-resistant Neisseria gonorrhoeae has prompted the development of rapid molecular assays designed to determine antimicrobial susceptibility. One common assay uses high-resolution melt analysis to target codon 91 of the gyrase A gene (gyrA) to predict N. gonorrhoeae susceptibility to ciprofloxacin.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We extracted DNA from remnant clinical specimens that had previously tested positive for N. gonorrhoeae using the Aptima Combo 2 for CT\/NG assay (Hologic, San Diego, CA, USA). We selected DNA extracts from specimens with indeterminate, WT and mutant gyrA genotype results from a previous study using high-resolution melt analysis to detect the gyrA codon 91 mutation. We re-tested those specimens using the recently CE-marked ResistancePlus GC (beta) assay (SpeeDx, Sydney, Australia).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Of 86 specimens with indeterminate gyrA genotypes on high-resolution melt analysis, the ResistancePlus GC (beta) assay (SpeeDx) identified 30 (35%) WT, 22 (26%) mutant and 34 (40%) indeterminate gyrA genotypes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The ResistancePlus GC (beta) assay showed improved N. gonorrhoeae gyrA genotype determination compared with a prior gyrA genotypic high-resolution melt assay.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkz292","type":"journal-article","created":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T11:08:39Z","timestamp":1560769719000},"page":"2913-2915","source":"Crossref","is-referenced-by-count":13,"title":["Improved determination of <i>Neisseria gonorrhoeae<\/i> gyrase A genotype results in clinical specimens"],"prefix":"10.1093","volume":"74","author":[{"given":"Lao-Tzu","family":"Allan-Blitz","sequence":"first","affiliation":[{"name":"Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA, USA"},{"name":"Department of Medicine, Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivia L","family":"Ellis","sequence":"additional","affiliation":[{"name":"Department of Environmental Health Sciences, Fielding School of Public Health, University of California, Los Angeles, CA, USA"},{"name":"Department of Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel","family":"Wee","sequence":"additional","affiliation":[{"name":"SpeeDx Pty Ltd, Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annie","family":"Truong","sequence":"additional","affiliation":[{"name":"SpeeDx Pty Ltd, Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha M","family":"Ebeyan","sequence":"additional","affiliation":[{"name":"SpeeDx Pty Ltd, Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lit Yeen","family":"Tan","sequence":"additional","affiliation":[{"name":"SpeeDx Pty Ltd, Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Mokany","sequence":"additional","affiliation":[{"name":"SpeeDx Pty Ltd, Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Risa","family":"Flynn","sequence":"additional","affiliation":[{"name":"Lesbian Gay Bisexual Transgender Health Center, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey D","family":"Klausner","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, David Geffen School of Medicine, University of California, Los Angeles, CA, USA"},{"name":"Department of Epidemiology, Fielding School of Public Health, University of California, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,7,24]]},"reference":[{"key":"2021061512511760700_dkz292-B1","doi-asserted-by":"crossref","first-page":"606","DOI":"10.7326\/0003-4819-148-8-200804150-00005","article-title":"Emerging antimicrobial resistance in Neisseria gonorrhoeae: urgent need to strengthen prevention 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