{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T23:20:46Z","timestamp":1790896846930,"version":"4.1.0"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T00:00:00Z","timestamp":1570060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"by Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico"},{"name":"INPRA - Instituto Nacional de Pesquisa em Resist\u00eancia Antimicrobiana\u2014Brazil","award":["465718\/2014-0"],"award-info":[{"award-number":["465718\/2014-0"]}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Programa de Excel\u00eancia em Pesquisa-CNPq"},{"DOI":"10.13039\/501100006507","name":"Funda\u00e7\u00e3o Oswaldo Cruz","doi-asserted-by":"crossref","award":["# APQ-1549-2.12\/15"],"award-info":[{"award-number":["# APQ-1549-2.12\/15"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"crossref"}]},{"name":"QB3\/Chemistry Mass Spectrometry Facility"},{"DOI":"10.13039\/100005595","name":"University of California","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1S10OD020062-01"],"award-info":[{"award-number":["1S10OD020062-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To identify the molecular mechanism of colistin resistance in an MDR Acinetobacter baumannii clinical strain isolated in 2008 from a meningitis case in Brazil.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Long- and short-read WGS was used to identify colistin resistance genes in A. baumannii strain 597A with a colistin MIC of 64\u2009mg\/L. MS was used to analyse lipid A content. mcr was cloned into pET-26b (+) and transformed into Escherichia coli BL21(\u03bbDE3)pLysS for analysis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>A novel plasmid (pAb-MCR4.3) harbouring mcr-4.3 within a Tn3-like transposon was identified. The A. baumannii 597A lipid A MS spectra showed a main molecular ion peak at m\/z=2034, which indicated the addition of phosphoethanolamine to the lipid A structure. E. coli BL21 transformed with pET-26b-mcr-4.3 gained colistin resistance with a colistin MIC of 8\u2009mg\/L.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Colistin resistance in A. baumannii 597A was correlated with the presence of a novel plasmid-encoded mcr-4.3 gene.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkz413","type":"journal-article","created":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T15:26:57Z","timestamp":1567697217000},"page":"60-64","source":"Crossref","is-referenced-by-count":87,"title":["A novel plasmid-encoded mcr-4.3 gene in a colistin-resistant Acinetobacter baumannii clinical strain"],"prefix":"10.1093","volume":"75","author":[{"given":"Natacha","family":"Martins-Sorenson","sequence":"first","affiliation":[{"name":"Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Snesrud","sequence":"additional","affiliation":[{"name":"Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo Elias","family":"Xavier","sequence":"additional","affiliation":[{"name":"Instituto Aggeu Magalh\u00e3es\/Fiocruz, Recife, Pernambuco, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Camila","family":"Cacci","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony T","family":"Iavarone","sequence":"additional","affiliation":[{"name":"QB3\/Chemistry Mass Spectrometry Facility, University of California, Berkeley, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"McGann","sequence":"additional","affiliation":[{"name":"Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee W","family":"Riley","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease and Vaccinology, School of Public Health, University of California, Berkeley, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1650-1382","authenticated-orcid":false,"given":"Beatriz Meurer","family":"Moreira","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,10,3]]},"reference":[{"key":"2019121310473088800_dkz413-B1","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1007\/s10096-018-3223-9","article-title":"Chromosomally encoded and plasmid-mediated polymyxins resistance in Acinetobacter baumannii: a huge public health threat","volume":"37","author":"Lima","year":"2018","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2019121310473088800_dkz413-B2","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1473-3099(15)00424-7","article-title":"Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study","volume":"16","author":"Liu","year":"2016","journal-title":"Lancet Infect 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