{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:04:26Z","timestamp":1790485466679,"version":"4.1.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T00:00:00Z","timestamp":1572480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Danish Research Agency (FNU-rammebevilling","award":["10-084554"],"award-info":[{"award-number":["10-084554"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine the mechanism of induction of erm(47) and its atypical expression in the Gram-positive opportunistic pathogen Helcococcus kunzii, where it confers resistance to a subset of clinically important macrolide, lincosamide and streptogramin B (MLSB) antibiotics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The resistant H. kunzii clinical isolate UCN99 was challenged with subinhibitory concentrations of a wide range of ribosome-targeting drugs. The methylation status of the H. kunzii ribosomal RNA at the MLSB binding site was then determined using an MS approach and was correlated with any increase in resistance to the drugs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The H. kunzii erm(47) gene encodes a monomethyltransferase. Expression is induced by subinhibitory concentrations of the macrolide erythromycin, as is common for many erm genes, and surprisingly also by 16-membered macrolide, lincosamide, streptogramin, ketolide, chloramphenicol and linezolid antibiotics, all of which target the 50S ribosomal subunit. No induction was detected with spectinomycin, which targets the 30S subunit.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The structure of the erm(47) leader sequence functions as a hair trigger for the induction mechanism that expresses resistance. Consequently, translation of the erm(47) mRNA is tripped by MLSB compounds and also by drugs that target the 50S ribosomal subunit outside the MLSB site. Expression of erm(47) thus extends previous assumptions about how erm genes can be induced.<\/jats:p><\/jats:sec>","DOI":"10.1093\/jac\/dkz441","type":"journal-article","created":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T12:50:26Z","timestamp":1570107026000},"source":"Crossref","is-referenced-by-count":3,"title":["Helcococcus kunzii methyltransferase Erm(47) responsible for MLSB resistance is induced by diverse ribosome-targeting antibiotics"],"prefix":"10.1093","author":[{"given":"Fran\u00e7ois","family":"Guerin","sequence":"first","affiliation":[{"name":"Service de Microbiologie, CHU de Caen, Avenue de la C\u00f4te de Nacre - CS30001 - 14033 Caen Cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Rose","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4026-9912","authenticated-orcid":false,"given":"Vincent","family":"Cattoir","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Hygi\u00e8ne hospitali\u00e8re & CNR de la R\u00e9sistance aux Antibiotiques (laboratoire associ\u00e9 \u2018Ent\u00e9rocoques\u2019), CHU de Rennes, 2 rue Henri Le Guilloux, 35033 Rennes Cedex 9, France"},{"name":"Unit\u00e9 Inserm U1230, Universit\u00e9 de Rennes 1, 2 avenue du Pr. L\u00e9on Bernard, 35043 Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5468-8486","authenticated-orcid":false,"given":"Stephen","family":"Douthwaite","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,10,31]]},"reference":[{"key":"2019103110193855600_dkz441-B1","volume-title":"Infections in the Immunocompromised Host","author":"Cohen","year":"2017"},{"key":"2019103110193855600_dkz441-B2","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1128\/JCM.35.11.2759-2761.1997","article-title":"Colonization of skin by Helcococcus kunzii","volume":"35","author":"Haas","year":"1997","journal-title":"J Clin 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