{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T05:13:23Z","timestamp":1772774003257,"version":"3.50.1"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T00:00:00Z","timestamp":1573516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Wu Jieping Medical Foundation of China","award":["320.6750.17339"],"award-info":[{"award-number":["320.6750.17339"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81602905"],"award-info":[{"award-number":["81602905"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To determine the association of Neisseria gonorrhoeae antimicrobial resistance and genotypes using N. gonorrhoeae sequence typing for antimicrobial resistance (NG-STAR).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We characterized 124 N. gonorrhoeae isolates for their antimicrobial susceptibility profiles and NG-STAR ST characteristics using the guidelines of CLSI and EUCAST. The NG-STAR STs of seven loci were analysed. N. gonorrhoeae multiantigen sequence typing (NG-MAST) and MLST analysis was conducted in isolates with specific NG-STAR STs.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>NG-STAR differentiated 124 N. gonorrhoeae isolates into 84 STs, of which 66 STs were novel to the NG-STAR database. NG-STAR ST-199, ST-348, ST-428, ST-497 and ST-1138 were the predominant STs. Three N. gonorrhoeae isolates with ceftriaxone and cefixime MICs \u22651.0\u2009mg\/L were grouped as NG-STAR ST-233. NG-STAR ST-202 isolates (n=4) were associated with high azithromycin MICs and had an identical NG-MAST ST. The NG-STAR ST-348 group (n=5) comprised more isolates with reduced susceptibility to cefixime (n=4) than cefixime-susceptible isolates (n=1).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>NG-STAR analysis differentiated N. gonorrhoeae isolates in settings with a high prevalence of antimicrobial resistance. Specific NG-STAR STs are associated with reduced susceptibility to ceftriaxone or cefixime and resistance to azithromycin in N. gonorrhoeae.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkz471","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T07:10:30Z","timestamp":1573110630000},"page":"566-570","source":"Crossref","is-referenced-by-count":14,"title":["NG-STAR genotypes are associated with MDR in Neisseria gonorrhoeae isolates collected in 2017 in Shanghai"],"prefix":"10.1093","volume":"75","author":[{"given":"Yijing","family":"Yang","sequence":"first","affiliation":[{"name":"Shanghai Skin Disease Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yang","sequence":"first","affiliation":[{"name":"Shanghai Skin Disease Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Martin","sequence":"first","affiliation":[{"name":"Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, MB, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Dong","sequence":"first","affiliation":[{"name":"Shanghai Municipal Center for Disease Control & Prevention, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nannan","family":"Diao","sequence":"first","affiliation":[{"name":"Shanghai Skin Disease Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wang","sequence":"first","affiliation":[{"name":"Shanghai Municipal Center for Disease Control & Prevention, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter","family":"Demczuk","sequence":"first","affiliation":[{"name":"Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, MB, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiming","family":"Gu","sequence":"first","affiliation":[{"name":"Shanghai Skin Disease Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,11,12]]},"reference":[{"key":"2020021410594576800_dkz471-B1","doi-asserted-by":"crossref","first-page":"731.","DOI":"10.1186\/s12879-014-0731-9","article-title":"Twenty-five-year changing pattern of gonococcal antimicrobial susceptibility in Shanghai: surveillance and its impact on treatment guidelines","volume":"14","author":"Gu","year":"2014","journal-title":"BMC Infect Dis"},{"key":"2020021410594576800_dkz471-B2","doi-asserted-by":"crossref","first-page":"e1002344.","DOI":"10.1371\/journal.pmed.1002344","article-title":"Antimicrobial resistance in Neisseria gonorrhoeae: global surveillance and a call for international collaborative action","volume":"14","author":"Wi","year":"2017","journal-title":"PLoS Med"},{"key":"2020021410594576800_dkz471-B3","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1093\/jac\/dkv321","article-title":"First nationwide study regarding ceftriaxone resistance and molecular epidemiology of Neisseria gonorrhoeae in China","volume":"71","author":"Chen","year":"2016","journal-title":"J Antimicrob Chemother"},{"key":"2020021410594576800_dkz471-B4","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1128\/CMR.00010-14","article-title":"Antimicrobial resistance in Neisseria gonorrhoeae in the 21st century: past, evolution, and future","volume":"27","author":"Unemo","year":"2014","journal-title":"Clin Microbiol Rev"},{"key":"2020021410594576800_dkz471-B5","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1093\/jac\/dkl301","article-title":"Antimicrobial susceptibility and molecular determinants of quinolone resistance in Neisseria gonorrhoeae isolates from Shanghai","volume":"58","author":"Yang","year":"2006","journal-title":"J Antimicrob Chemother"},{"key":"2020021410594576800_dkz471-B6","doi-asserted-by":"crossref","first-page":"4327","DOI":"10.1128\/AAC.49.10.4327-4334.2005","article-title":"High-level chromosomally mediated tetracycline resistance in Neisseria gonorrhoeae results from a point mutation in the rpsJ gene encoding ribosomal protein S10 in combination with the mtrR and penB resistance determinants","volume":"49","author":"Hu","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"2020021410594576800_dkz471-B7","doi-asserted-by":"crossref","first-page":"3812","DOI":"10.1128\/AAC.00309-10","article-title":"High-level azithromycin resistance occurs in Neisseria gonorrhoeae as a result of a single point mutation in the 23S rRNA genes","volume":"54","author":"Chisholm","year":"2010","journal-title":"Antimicrob Agents 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