{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:39:51Z","timestamp":1791319191690,"version":"4.3.1"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T00:00:00Z","timestamp":1611360000000},"content-version":"vor","delay-in-days":366,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100005869","name":"University of Fribourg","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005869","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["FNS-31003A_163432"],"award-info":[{"award-number":["FNS-31003A_163432"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["FNS-407240_177381"],"award-info":[{"award-number":["FNS-407240_177381"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"INSERM","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006129","name":"FCT","doi-asserted-by":"publisher","award":["PTDC\/DTP-EPI\/0842\/2014"],"award-info":[{"award-number":["PTDC\/DTP-EPI\/0842\/2014"]}],"id":[{"id":"10.13039\/100006129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002924","name":"FEDER","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]},{"name":"COMPETE2020 - Programa Operacional Competitividade e Internacionaliza\u00e7\u00e3o"},{"name":"POCI","award":["LISBOA-01\u20130145-FEDER-007660"],"award-info":[{"award-number":["LISBOA-01\u20130145-FEDER-007660"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To decipher the genetics of acquisition of carbapenemase-encoding genes identified in two carbapenem-resistant Enterobacteriaceae recovered from a single patient in Portugal.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Carbapenemase genes were searched by PCR assays and mating-out assays were performed to further characterize the plasmid support of the carbapenemase genes. Genetic characterization of the plasmid supports was performed by whole-plasmid sequencing using the Illumina technology.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We identified here two NDM-1-producing isolates, namely a Morganella morganii and a Proteus mirabilis, sharing the same blaNDM-1-positive plasmid. This 154\u2009kb plasmid belonged to the IncA\/C2 type, recently renamed IncC, and co-harboured two AmpC \u03b2-lactamase genes, namely blaCMY-4 and blaDHA-1, in addition to the 16S rRNA methylase gene armA encoding high-level resistance to aminoglycosides. In addition, the M. morganii isolate produced the CTX-M-33 extended-spectrum \u03b2-lactamase possessing weak carbapenemase activity, encoded by another plasmid.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We showed here that, in addition to KPC-type and OXA-181 carbapenemases, which have been identified as widespread in this country, another concern is the emergence of NDM-1-producing enterobacterial isolates in Portugal. We demonstrated here the in vivo plasmid transfer of a blaNDM-1-positive plasmid leading to dissemination of this carbapenemase gene within different enterobacterial species in a single patient.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkz542","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T20:10:33Z","timestamp":1576008633000},"page":"903-906","source":"Crossref","is-referenced-by-count":35,"title":["Occurrence of NDM-1-producing <i>Morganella morganii<\/i> and <i>Proteus mirabilis<\/i> in a single patient in Portugal: probable <i>in vivo<\/i> transfer by conjugation"],"prefix":"10.1093","volume":"75","author":[{"given":"Marta","family":"Aires-de-Sousa","sequence":"first","affiliation":[{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland"},{"name":"Escola Superior de Sa\u00fade da Cruz Vermelha Portuguesa (ESSCVP), Lisboa, Portugal"},{"name":"Laboratory of Molecular Genetics, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier (ITQB), Universidade Nova de Lisboa (UNL), Oeiras, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Manuel","family":"Ortiz de la Rosa","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Lu\u00edsa","family":"Goncalves","sequence":"additional","affiliation":[{"name":"Hospital SAMS, Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augusto","family":"Costa","sequence":"additional","affiliation":[{"name":"Hospital SAMS, Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland"},{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland"},{"name":"Escola Superior de Sa\u00fade da Cruz Vermelha Portuguesa (ESSCVP), Lisboa, Portugal"},{"name":"Laboratory of Molecular Genetics, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier (ITQB), Universidade Nova de Lisboa (UNL), Oeiras, Portugal"},{"name":"Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland"},{"name":"Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland"},{"name":"INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,1,23]]},"reference":[{"key":"2021012220111310700_dkz542-B1","doi-asserted-by":"crossref","first-page":"249856.","DOI":"10.1155\/2014\/249856","article-title":"Worldwide dissemination of the NDM-type carbapenemases in Gram-negative bacteria","volume":"2014","author":"Dortet","year":"2014","journal-title":"BioMed Res 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