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Further modifying this strain to express NanoLuc created a dual reporter strain capable of quantifying parasites in both the trophozoite and cyst stages. These strains were used to develop a high-throughput cell assay and a mouse infection model. A library of MetRS inhibitors was screened in the cell assay and Compound-1717 was tested for efficacy in the mouse infection model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Cell viability in in vitro compound screens was quantified via bioluminescence readouts while infection loads in mice were monitored with non-invasive whole-animal imaging and faecal analysis. Compound-1717 was effective in clearing mice of Giardia infection in 3\u2009days at varying doses, which was supported by data from enzymatic and phenotypic cell assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The new in vitro and in vivo assays based on luciferase expression by engineered G. lamblia strains are useful for the discovery and development of new therapeutics for giardiasis. MetRS inhibitors, as validated by Compound-1717, have promising anti-giardiasis properties that merit further study as alternative therapeutics.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkz567","type":"journal-article","created":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T15:10:17Z","timestamp":1576854617000},"page":"1218-1227","source":"Crossref","is-referenced-by-count":17,"title":["Methionyl-tRNA synthetase inhibitor has potent\n                    <i>in vivo<\/i>\n                    activity in a novel\n                    <i>Giardia lamblia<\/i>\n                    luciferase murine infection model"],"prefix":"10.1093","volume":"75","author":[{"given":"Samantha A","family":"Michaels","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington, Seattle, WA 98109, 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