{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T06:36:43Z","timestamp":1774766203143,"version":"3.50.1"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T00:00:00Z","timestamp":1632182400000},"content-version":"vor","delay-in-days":71,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"German Federal Institute for Risk Assessment"},{"name":"German One Health Initiative","award":["1322-687"],"award-info":[{"award-number":["1322-687"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Human health surveillance and food safety monitoring systems use different antimicrobial susceptibility testing (AST) methods. In this study, we compared the MICs of Escherichia coli isolates provided by these methods.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>E. coli isolates (n = 120) from human urine samples and their MICs were collected from six medical laboratories that used automated AST methods based on bacterial growth kinetic analyses. These isolates were retested using broth microdilution, which is used by the food safety monitoring system. The essential and categorical agreements (EA and CA), very major errors (VME), major errors (ME) and minor errors (mE) for these two methods were calculated for 11 antibiotics using broth microdilution as a reference. For statistical analysis, clinical breakpoints provided by EUCAST were used.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Five study laboratories used VITEK\u00ae2 and one MicroScan (Walkaway Combo Panel). Out of 120 isolates, 118 isolates (98.3%) were confirmed as E. coli. The 99 E. coli isolates from five study laboratories that used VITEK\u00ae2 showed high proportions of EA and CA with full agreements for gentamicin, meropenem, imipenem and ertapenem. Additionally, 100% CA was also observed in cefepime. Few VME (0.5%), ME (1.9%) and mE (1.5%) were observed across all antibiotics. One VME for ceftazidime (7.1%) and 12 MEs for ampicillin (29.4%), cefotaxime (2.4%), ciprofloxacin (3.2%), tigecycline (1.5%) and trimethoprim (22.2%) were detected.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>MICs from E. coli isolates produced by VITEK\u00ae2 were similar to those determined by broth microdilution. These results will be valuable for comparative analyses of resistance data from human health surveillance and food safety monitoring systems.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jacamr\/dlab145","type":"journal-article","created":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T11:11:30Z","timestamp":1631531490000},"source":"Crossref","is-referenced-by-count":3,"title":["Comparison of MICs in <i>Escherichia coli<\/i> isolates from human health surveillance with MICs obtained for the same isolates by broth microdilution"],"prefix":"10.1093","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5021-6510","authenticated-orcid":false,"given":"Beneditta","family":"Suwono","sequence":"first","affiliation":[{"name":"Unit Epidemiology, Zoonoses and Antimicrobial Resistance, Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany"},{"name":"Division of Healthcare-associated Infections, Surveillance of Antibiotic Resistance and Consumption, Department Infectious of Disease Epidemiology, Robert Koch Institute, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6930-4358","authenticated-orcid":false,"given":"Jens Andr\u00e9","family":"Hammerl","sequence":"additional","affiliation":[{"name":"Unit Epidemiology, Zoonoses and Antimicrobial Resistance, Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Eckmanns","sequence":"additional","affiliation":[{"name":"Division of Healthcare-associated Infections, Surveillance of Antibiotic Resistance and Consumption, Department Infectious of Disease Epidemiology, Robert Koch Institute, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roswitha","family":"Merle","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Institute for Veterinary Epidemiology and Biostatistics, Free University Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Eigner","sequence":"additional","affiliation":[{"name":"Study Department for Infectious Diseases, MVZ Labor Dr. Limbach & Kollegen GbR, Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"L\u00fcmen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Labor M\u00f6nchengladbach MVZ Dr Stein + Kollegen DbR, M\u00f6nchengladbach, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sven","family":"Lauter","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, LADR GmbH MVZ Nord, Flintbek, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00fcdiger","family":"Stock","sequence":"additional","affiliation":[{"name":"Department of Microbiology, SYNLAB MVZ Berlin GmbH, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ines","family":"Fenner","sequence":"additional","affiliation":[{"name":"Labor Dr. Fenner & Kollegen MVZ, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Boemke","sequence":"additional","affiliation":[{"name":"Zentrallabor FEK\u2014Friedrich-Ebert-Krankenhaus Neum\u00fcnster GmbH, Neum\u00fcnster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2191-7569","authenticated-orcid":false,"given":"Bernd-Alois","family":"Tenhagen","sequence":"additional","affiliation":[{"name":"Unit Epidemiology, Zoonoses and Antimicrobial Resistance, Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,9,21]]},"reference":[{"key":"2021092105512945300_dlab145-B1","doi-asserted-by":"crossref","first-page":"E466","DOI":"10.1111\/j.1469-0691.2012.03996.x","article-title":"Clinical breakpoint changes and their impact on surveillance of antimicrobial resistance in Escherichia coli causing bacteraemia","volume":"18","author":"van der Bij","year":"2012","journal-title":"Clin Microbiol Infect"},{"key":"2021092105512945300_dlab145-B2","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/s41579-018-0098-9","article-title":"Developmental roadmap for antimicrobial susceptibility testing systems","volume":"17","author":"van Belkum","year":"2019","journal-title":"Nat Rev Microbiol"},{"key":"2021092105512945300_dlab145-B3","author":"EUCAST"},{"key":"2021092105512945300_dlab145-B4","author":"bioM\u00e9rieux"},{"key":"2021092105512945300_dlab145-B5","author":"bioM\u00e9rieux"},{"key":"2021092105512945300_dlab145-B6","author":"European Commission"},{"key":"2021092105512945300_dlab145-B7","author":"bioM\u00e9rieux. VITEK 2 - Karten f\u00fcr die Empfindlichkeitspr\u00fcfung. bioM\u00e9rieux","year":"2019"},{"key":"2021092105512945300_dlab145-B8","author":"Federal Ministry of Health. DART 2020: Fighting Antibiotic Resistance for the Good of Both Humans and Animals. Federal Ministry of Health","year":"2015"},{"key":"2021092105512945300_dlab145-B9","author":"Robert Koch Institute (RKI)"},{"key":"2021092105512945300_dlab145-B10","author":"EUCAST","year":"2019"},{"key":"2021092105512945300_dlab145-B11","doi-asserted-by":"crossref","first-page":"i24","DOI":"10.1093\/jac\/dky532","article-title":"Antimicrobial susceptibility testing of pathogens isolated from blood culture: a performance comparison of Accelerate Pheno\u2122 and VITEK\u00ae 2 systems with the broth microdilution method","volume":"74","author":"De Angelis","year":"2019","journal-title":"J Antimicrob Chemother"},{"key":"2021092105512945300_dlab145-B12","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1128\/JCM.02697-14","article-title":"Performance of Vitek 2 for antimicrobial susceptibility testing of Enterobacteriaceae with Vitek 2 (2009 FDA) and 2014 CLSI breakpoints","volume":"53","author":"Bobenchik","year":"2015","journal-title":"J Clin Microbiol"},{"key":"2021092105512945300_dlab145-B13","author":"bioM\u00e9rieux"},{"key":"2021092105512945300_dlab145-B14","first-page":"2742","article-title":"Technical specifications on the harmonised monitoring and reporting of antimicrobial resistance in Salmonella, Campylobacter and indicator Escherichia coli and Enterococcus spp. bacteria transmitted through food","volume":"10","author":"European Food Safety Authority (EFSA)","year":"2012","journal-title":"EFSA J"},{"key":"2021092105512945300_dlab145-B15","first-page":"e05709","article-title":"Technical specifications on harmonised monitoring of antimicrobial resistance in zoonotic and indicator bacteria from food-producing animals and food","volume":"17","author":"Aerts","year":"2019","journal-title":"EFSA J"}],"container-title":["JAC-Antimicrobial Resistance"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jacamr\/article-pdf\/3\/3\/dlab145\/40418424\/dlab145.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jacamr\/article-pdf\/3\/3\/dlab145\/40418424\/dlab145.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T05:52:25Z","timestamp":1632203545000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jacamr\/article\/doi\/10.1093\/jacamr\/dlab145\/6373001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,12]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,7,12]]}},"URL":"https:\/\/doi.org\/10.1093\/jacamr\/dlab145","relation":{},"ISSN":["2632-1823"],"issn-type":[{"value":"2632-1823","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,9,1]]},"published":{"date-parts":[[2021,7,12]]},"article-number":"dlab145"}}