{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T19:56:47Z","timestamp":1793303807144,"version":"4.5.0"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T00:00:00Z","timestamp":1644364800000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Cefiderocol is a siderophore cephalosporin active against MDR Gram-negatives including Stenotrophomonas maltophilia. Cefiderocol resistance remains uncommon and incompletely understood. We selected for cefiderocol-resistant S. maltophilia in vitro and characterized the genetic mechanisms and potential for cross-resistance to other antimicrobials.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We selected cefiderocol resistance in three clinical strains of S. maltophilia by serial passage in escalating concentrations of cefiderocol. Emergent cefiderocol-resistant isolates were subjected to repeat susceptibility testing against a panel of relevant antimicrobials. Isolates with confirmed MIC changes were whole genome sequenced.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Each parent strain was initially susceptible to cefiderocol (MICs of 0.03125, 0.03125 and 0.125\u2005mg\/L), and one initially tested susceptible to ceftazidime\/avibactam (MIC 4\u2005mg\/L). We recovered evolved isolates achieving cefiderocol resistance at MICs of 8\u201332\u2005mg\/L from each parental strain. Some cefiderocol resistant isolates reverted following one to four drug-free passages. Ceftazidime\/avibactam MICs of passaged isolates repeatedly increased to \u2265256\u2005mg\/L, and while other MICs were largely unchanged, trimethoprim\/sulfamethoxazole MICs declined 4-fold in two strains. WGS revealed one evolved isolate carrying six coding mutations, while four were isogenic mutants of tonB, tolQ, smf-1 and the smeT promoter. Mutation of the smeT promoter downregulated the smeDEF efflux pump and reduced susceptibility to penicillins but increased susceptibility to several other classes including sulphonamides. Other mutations occurred in genes putatively involved in iron metabolism including smlt1148 and cirA.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>S. maltophilia strains evolved cefiderocol resistance through different genetic pathways, but often involved iron transport. Future work is required to fully understand the role(s) of other genes in cefiderocol resistance.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jacamr\/dlac011","type":"journal-article","created":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T20:12:28Z","timestamp":1643400748000},"source":"Crossref","is-referenced-by-count":32,"title":["Evolution of cefiderocol resistance in <i>Stenotrophomonas maltophilia<\/i> using <i>in vitro<\/i> serial passage techniques"],"prefix":"10.1093","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6751-4098","authenticated-orcid":false,"given":"Brian J.","family":"Werth","sequence":"first","affiliation":[{"name":"Department of Pharmacy, School of Pharmacy, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel K.","family":"Ashford","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmacy, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsi","family":"Penewit","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, School of Medicine, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Waalkes","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, School of Medicine, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Holmes","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, School of Medicine, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Bryan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, School of Medicine, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Salipante","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, School of Medicine, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,2,8]]},"reference":[{"key":"2022031017581810900_dlac011-B1","doi-asserted-by":"crossref","first-page":"e00093-17","DOI":"10.1128\/AAC.00093-17","article-title":"In vitro activity of the siderophore cephalosporin, cefiderocol, against a recent collection of clinically relevant Gram-negative bacilli from North America and Europe, including carbapenem-nonsusceptible isolates (SIDERO-WT-2014 study)","volume":"61","author":"Hackel","year":"2017","journal-title":"Antimicrob Agents 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