{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:01:53Z","timestamp":1792450913025,"version":"4.5.0"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"vor","delay-in-days":14,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>We investigated the amino acid substitutions in the GES family of ESBLs that were most likely to be involved in the evolution of carbapenemase activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To identify the substitutions that are functionally important, we analysed the evolutionary history of the GES \u03b2-lactamases using an alignment and phylogeny to identify sites in GES that show evidence of positive selection and the selected phenotypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Conclusions<\/jats:title>\n                    <jats:p>Data indicate that the substitutions G170S and G243A are associated with carbapenemase activity. The substitutions Q43E, E104K and T237A are most likely associated with ESBL activity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jacamr\/dlad150","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T00:47:23Z","timestamp":1704761243000},"source":"Crossref","is-referenced-by-count":11,"title":["Phylogenetic predictions of carbapenemase activity from the Guiana extended-spectrum (GES) family of \u03b2-lactamases"],"prefix":"10.1093","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7349-190X","authenticated-orcid":false,"given":"Miriam","family":"Barlow","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California , Merced, CA 95343 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred C","family":"Tenover","sequence":"additional","affiliation":[{"name":"College of Arts and Sciences: Biology, University of Dayton , Dayton, OH 45469 , 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