{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:04:14Z","timestamp":1753877054593,"version":"3.41.2"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T00:00:00Z","timestamp":1703721600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011777","name":"Scandinavian Society for Antimicrobial Chemotherapy (SSAC) Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011777","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Regional Medical and Treatment Foundation"},{"DOI":"10.13039\/100009838","name":"Independent Research Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009838","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To determine if vancomycin-resistant Enterococcus faecium (VREfm) carriers carry the same VREfm clone after a minimum follow-up of 365\u2005days. For those carrying the same clone, we investigated the genomic evolution per year per genome.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We used WGS results to assign VREfm clones to each isolate and determine clone shifts. Finally, we calculated distance in core-genome MLST alleles, and the number of SNPs between consecutive VREfm isolates from patients carrying the same VREfm clone.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In total, 44.2% of patients carried the same VREfm clone, and the genomic evolution was 1.8 alleles and 2.6 SNPs per genome per year.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>In our population of long-term carriers, we calculated a molecular clock of 2.6 SNPs.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jacamr\/dlad153","type":"journal-article","created":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T12:44:17Z","timestamp":1703767457000},"source":"Crossref","is-referenced-by-count":0,"title":["Long-term carriage and evolution of vancomycin-resistant <i>Enterococcus faecium<\/i>: a genomic study on consecutive isolates"],"prefix":"10.1093","volume":"6","author":[{"given":"Maja Johanne S\u00f8ndergaard","family":"Knudsen","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital\u2014Amager and Hvidovre , Hvidovre , Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose Alfredo","family":"Samaniego Castruita","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital\u2014Amager and Hvidovre , Hvidovre , Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2429-4018","authenticated-orcid":false,"given":"Sarah","family":"Mollerup","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital\u2014Amager and Hvidovre , Hvidovre , Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara Juliane","family":"Holzknecht","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital\u2014Herlev and Gentofte , Herlev , Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen , Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morten","family":"Hoppe","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital\u2014Herlev and Gentofte , Herlev , Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik","family":"Westh","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital\u2014Amager and Hvidovre , Hvidovre , Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences, University 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