{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T10:51:05Z","timestamp":1785149465130,"version":"3.55.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":29,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001505","name":"Health Research Council of New Zealand","doi-asserted-by":"publisher","award":["22\/323"],"award-info":[{"award-number":["22\/323"]}],"id":[{"id":"10.13039\/501100001505","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001505","name":"Health Research Council of New Zealand","doi-asserted-by":"publisher","award":["22\/156"],"award-info":[{"award-number":["22\/156"]}],"id":[{"id":"10.13039\/501100001505","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>A limited ability to eliminate drug-resistant strains of Mycobacterium tuberculosis is a major contributor to the morbidity of TB. Complicating this problem, little is known about how drug resistance-conferring mutations alter the ability of M. tuberculosis to tolerate antibiotic killing. Here, we investigated if drug-resistant strains of M. tuberculosis have an altered ability to tolerate killing by cell wall-targeting inhibitors.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Bacterial killing and MIC assays were used to test for antibiotic tolerance and synergy against a panel of drug-resistant M. tuberculosis strains.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Our results demonstrate that vancomycin and thioacetazone exhibit increased killing of diverse drug-resistant strains. Mutations in mmaA4 and mmpL3 increased vancomycin killing, which was consistent with vancomycin synergizing with thioacetazone and MmpL3-targeting inhibitors. In contrast, mutations in the mce1 operon conferred tolerance to vancomycin.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Overall, this work demonstrates how drug-resistant strains experience perturbations in cell-wall production that alters their tolerance to killing by cell wall-targeting inhibitors.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jacamr\/dlae086","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T05:00:35Z","timestamp":1717477235000},"source":"Crossref","is-referenced-by-count":10,"title":["Antibiotic resistance in <i>Mycobacterium tuberculosis<\/i> alters tolerance to cell wall-targeting inhibitors"],"prefix":"10.1093","volume":"6","author":[{"given":"William J","family":"Jowsey","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Otago , Dunedin , New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory M","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Otago , Dunedin , New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew B","family":"McNeil","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Otago , Dunedin , New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,6,4]]},"reference":[{"key":"2024062806361029500_dlae086-B1","author":"WHO"},{"key":"2024062806361029500_dlae086-B2","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1093\/cid\/cix992","article-title":"Bedaquiline resistance: its emergence, mechanism, and prevention","volume":"66","author":"Van Anh Nguyen","year":"2018","journal-title":"Clin Infect Dis"},{"key":"2024062806361029500_dlae086-B3","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1093\/jac\/dkab505","article-title":"Delamanid or pretomanid? A Solomonic judgement!","volume":"77","author":"Mudde","year":"2022","journal-title":"J Antimicrob Chemother"},{"key":"2024062806361029500_dlae086-B4","doi-asserted-by":"crossref","first-page":"e01439-20","DOI":"10.1128\/AAC.01439-20","article-title":"Most-probable-number-based minimum duration of killing assay for determining the spectrum of rifampicin susceptibility in clinical Mycobacterium tuberculosis isolates","volume":"65","author":"Vijay","year":"2021","journal-title":"Antimicrob Agents Chemother"},{"key":"2024062806361029500_dlae086-B5","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/s41579-019-0273-7","article-title":"The mycobacterial cell envelope\u2014a moving target","volume":"18","author":"Dulberger","year":"2020","journal-title":"Nat Rev Microbiol"},{"key":"2024062806361029500_dlae086-B6","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.mib.2021.01.012","article-title":"Synthesis and recycling of the mycobacterial cell 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