{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T01:47:05Z","timestamp":1788918425209,"version":"build-2803163510"},"reference-count":9,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011777","name":"Scandinavian Society for Antimicrobial Chemotherapy (SSAC) Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011777","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Regions\u2019 Medicines and Treatment Foundation"},{"DOI":"10.13039\/100009838","name":"Independent Research Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009838","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To develop and validate a real-time PCR assay detecting the sequence bridging Tn1549 and the Enterococcus faecium chromosome in the emerging vanB vancomycin-resistant E. faecium (VREfm) clone (ST80\/CT2406).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The Tn1549 insertion site was determined on routinely sequenced VREfm isolates. The outer boundaries of Tn1549 and adjoining host bacterial sequences were determined using a BLAST search in the silent information regulator gene sir2. Next, the primers and probe were developed, targeting the sequence bridging Tn1549 and the E. faecium chromosome. Finally, the PCR assay was validated on well-characterized strains and prospectively performed on rectal screening samples submitted to our laboratory.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results and conclusions<\/jats:title>\n                  <jats:p>The PCR assay proved to be accurate and provide rapid diagnosis of the emerging vanB VREfm in rectal screening samples.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jacamr\/dlae180","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T06:59:00Z","timestamp":1730962740000},"source":"Crossref","is-referenced-by-count":4,"title":["Development of a PCR assay for rapid and accurate detection of an emerging <i>vanB Enterococcus faecium<\/i> clone in the Capital Region of Denmark"],"prefix":"10.1093","volume":"6","author":[{"given":"Maja Johanne S\u00f8ndergaard","family":"Knudsen","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre , Hvidovre ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christel","family":"Barker Jensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre , Hvidovre ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikke Lind","family":"J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre , Hvidovre ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas Munk","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre , Hvidovre ,","place":["Denmark"]},{"name":"Gastrounit, Medical Division, Copenhagen University Hospital Hvidovre , Hvidovre ,","place":["Denmark"]},{"name":"Department of Clinical Medicine, University of Copenhagen , Copenhagen ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gitte","family":"Qvist Kristiansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre , Hvidovre ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Gorm","family":"Lisby","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre , Hvidovre ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peder","family":"Worning","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre , Hvidovre ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Westh","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre , Hvidovre ,","place":["Denmark"]},{"name":"Department of Clinical Medicine, University of Copenhagen , Copenhagen ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette","family":"Pinholt","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre , Hvidovre ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,11,7]]},"reference":[{"key":"2024122015564451000_dlae180-B1","doi-asserted-by":"publisher","first-page":"19046","DOI":"10.2807\/ese.13.47.19046-en","article-title":"Emergence and spread of vancomycin resistance among enterococci in Europe","volume":"13","author":"Werner","year":"2008","journal-title":"Euro Surveill"},{"key":"2024122015564451000_dlae180-B2","doi-asserted-by":"publisher","first-page":"2184","DOI":"10.1093\/jac\/dkx138","article-title":"Emergence of vanA Enterococcus faecium in Denmark, 2005\u201315","volume":"72","author":"Hammerum","year":"2017","journal-title":"J Antimicrob Chemother"},{"key":"2024122015564451000_dlae180-B3","article-title":"DANMAP 2021. Use of Antimicrobial Agents and Occurrence of Antimicrobial Resistance in Bacteria from Food Animals, Food and Humans in Denmark","author":"DANMAP","year":"2021"},{"key":"2024122015564451000_dlae180-B4","doi-asserted-by":"publisher","first-page":"2260","DOI":"10.1093\/jac\/dkab198","article-title":"Investigation of the introduction and dissemination of vanB Enterococcus faecium in the Capital Region of Denmark and development of a rapid and accurate clone-specific vanB E. faecium PCR","volume":"76","author":"Pinholt","year":"2021","journal-title":"J Antimicrob Chemother"},{"key":"2024122015564451000_dlae180-B5","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1093\/jac\/dkw360","article-title":"Genomic analysis of 495 vancomycin-resistant Enterococcus faecium reveals broad dissemination of a vanA plasmid in more than 19 clones from Copenhagen, Denmark","volume":"72","author":"Pinholt","year":"2017","journal-title":"J Antimicrob Chemother"},{"key":"2024122015564451000_dlae180-B6","doi-asserted-by":"publisher","first-page":"1776","DOI":"10.1093\/jac\/dkz118","article-title":"WGS of 1058 Enterococcus faecium from Copenhagen, Denmark, reveals rapid clonal expansion of vancomycin-resistant clone ST80 combined with widespread dissemination of a vanA-containing plasmid and acquisition of a heterogeneous accessory genome","volume":"74","author":"Pinholt","year":"2019","journal-title":"J Antimicrob Chemother"},{"key":"2024122015564451000_dlae180-B7","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1186\/s13059-018-1540-z","article-title":"SKESA: strategic k-mer extension for scrupulous assemblies","volume":"19","author":"Souvorov","year":"2018","journal-title":"Genome Biol"},{"key":"2024122015564451000_dlae180-B8","doi-asserted-by":"publisher","first-page":"3788","DOI":"10.1128\/JCM.01946-15","article-title":"Core genome multilocus sequence typing scheme for high-resolution typing of Enterococcus faecium","volume":"53","author":"de Been","year":"2015","journal-title":"J Clin Microbiol"},{"key":"2024122015564451000_dlae180-B9","doi-asserted-by":"publisher","first-page":"3259","DOI":"10.1093\/jac\/dky349","article-title":"Elucidating vancomycin-resistant Enterococcus faecium outbreaks: the role of clonal spread and movement of mobile genetic elements","volume":"73","author":"Zhou","year":"2018","journal-title":"J Antimicrob Chemother"}],"container-title":["JAC-Antimicrobial Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jacamr\/article-pdf\/6\/6\/dlae180\/61219950\/dlae180.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jacamr\/article-pdf\/6\/6\/dlae180\/61219950\/dlae180.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T15:56:55Z","timestamp":1734710215000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jacamr\/article\/doi\/10.1093\/jacamr\/dlae180\/7882827"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,30]]},"references-count":9,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,10,30]]}},"URL":"https:\/\/doi.org\/10.1093\/jacamr\/dlae180","relation":{},"ISSN":["2632-1823"],"issn-type":[{"value":"2632-1823","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,12]]},"published":{"date-parts":[[2024,10,30]]},"article-number":"dlae180"}}