{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:23:04Z","timestamp":1791084184571,"version":"4.1.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":15,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260435"],"award-info":[{"award-number":["82260435"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Second Batch of the Tianshan Talent Cultivation Program for Young Promising Talents","award":["2023TSYCQNTJ0003"],"award-info":[{"award-number":["2023TSYCQNTJ0003"]}]},{"name":"Key Laboratory of High Incidence Disease Research in Xinjiang"},{"name":"Ministry of Education-Key project","award":["2023A01"],"award-info":[{"award-number":["2023A01"]}]},{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia","award":["SKL-HIDCA-2021-9"],"award-info":[{"award-number":["SKL-HIDCA-2021-9"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Periprosthetic joint infection (PJI) is a significant complication following arthroplasty, attributed to the biofilm formation. This study evaluates the effectiveness of vancomycin-loaded microbubbles (Van-MBs) in conjunction with ultrasound-targeted microbubble destruction (UTMD) on biofilm disruption and bactericidal efficiency.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Van-MBs were prepared using the thin-film hydration method and characterized using microscopy, dynamic light scattering analysis, and high-performance liquid chromatography (HPLC). Confocal laser scanning microscopy (CLSM) was used to assess the penetration of Van and Van-MBs into biofilms. Biofilms were treated with Van, Van-MBs, UTMD, and Van-MBs\u200a+\u200aUTMD. CLSM and crystal violet staining were utilized to assess the morphology, viability, and biomass of the biofilms. Bacterial activity was examined through scanning electron microscopy (SEM) and plate counting, while gene expression was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The results demonstrated that Van-MBs penetrated deeper into methicillin-resistant Staphylococcus aureus (MRSA) biofilms compared with Van alone. The combination of Van-MBs and UTMD significantly reduced biofilm thickness, viability, and biomass. qRT\u2013PCR analysis revealed that the Van-MBs\u200a+\u200aUTMD group exhibited lower transcription levels of the icaA gene, suggesting that the treatment disrupted biofilm formation by suppressing this key gene. SEM further confirmed the efficacy of the treatment, showing that Van-MBs\u200a+\u200aUTMD induced cytoplasmic shrinkage and separation of the outer and cytoplasmic membranes in MRSA cells, indicating substantial structural damage to the bacterial cells.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>These findings demonstrate the potential of Van-MBs in combination with UTMD as an innovative approach to enhance antibiotic efficacy and eliminate biofilms in the treatment of PJI.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jacamr\/dlaf045","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T11:26:11Z","timestamp":1742383571000},"source":"Crossref","is-referenced-by-count":17,"title":["Enhancing biofilm disruption and bactericidal efficiency using vancomycin-loaded microbubbles in sonodynamic therapy"],"prefix":"10.1093","volume":"7","author":[{"given":"Wen B","family":"Mu","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, School of Pharmacy, Xinjiang Medical University , Urumqi, Xinjiang 830011 ,","place":["China"]},{"name":"Department of Orthopaedics, First Affiliated Hospital of Xinjiang Medical University , Urumqi, Xinjiang 830054 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li Q","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, First Affiliated Hospital of Xinjiang Medical University , Urumqi, Xinjiang 830054 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi Y","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, First Affiliated Hospital of Xinjiang Medical University , Urumqi, Xinjiang 830054 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You C","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, First Affiliated Hospital of Xinjiang Medical University , Urumqi, Xinjiang 830054 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, First Affiliated Hospital of Xinjiang Medical University , Urumqi, Xinjiang 830054 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian H","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, School of Pharmacy, Xinjiang Medical University , Urumqi, Xinjiang 830011 ,","place":["China"]},{"name":"Department of Pharmacy, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, The First Affiliated Hospital of Xinjiang Medical University , Urumqi, Xinjiang 830011 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