{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T09:24:09Z","timestamp":1793352249204,"version":"4.5.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":35,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Pseudomonas aeruginosa is one of the major drivers of morbidity and mortality in patients with chronic underlying diseases. Whereas cystic fibrosis (CF) P. aeruginosa strains have been well studied, non-CF bronchiectasis isolates have received less scientific attention.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We determined the antibiotic susceptibility profiles of a collection of 100 P. aeruginosa isolates recovered from a total of 100 non-CF bronchiectasis patients attending a Catalonian hospital. All carbapenemase-producing isolates were characterized by WGS.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Twelve isolates were classified as MDR (12%) and six were found to be carbapenemase (VIM-2) producers (6%). Of note, two of the VIM-2-producing isolates were carbapenem susceptible due to the presence of inactivating mutations in MexAB-OprM efflux pump components. These isolates exhibited properties of chronic P. aeruginosa isolates, such as mutator or mucoid phenotypes that are associated with persistent infections despite intensive antibiotic therapies. The phylogenetic analysis evidenced that all VIM-2 isolates belonged to the high-risk clone ST235. Core-genome MLST analysis revealed 7\u2013260 allelic differences, arguing against recent transmission but a common source of infection or an ancient interpatient transmission event could not be ruled out.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Altogether, these findings suggest that P. aeruginosa chronic respiratory infections can be an important and silent reservoir of transferable resistance determinants and P. aeruginosa high-risk clones, thus contributing to their increased resistance and worldwide dissemination.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jacamr\/dlaf053","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T16:46:20Z","timestamp":1744130780000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Pseudomonas aeruginosa<\/i> chronic infections in patients with bronchiectasis: a silent reservoir of carbapenemase-producing epidemic high-risk clones"],"prefix":"10.1093","volume":"7","author":[{"given":"Alba","family":"Mu\u00f1oz-Santa","sequence":"first","affiliation":[{"name":"Servicio de An\u00e1lisis Cl\u00ednicos, Hospital Universitari Arnau de Vilanova de Lleida, Institut de Recerca Biom\u00e8dica de Lleida (IRB Lleida), Universitat de Lleida , Lleida ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2228-2005","authenticated-orcid":false,"given":"Carla","family":"L\u00f3pez-Causap\u00e9","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Univeristario Son Espases, Instituto de Investigaci\u00f3n Sanitaria Illes Balears, CIBERINFEC , Palma de Mallorca ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Bell\u00e9s","sequence":"additional","affiliation":[{"name":"Servicio de An\u00e1lisis Cl\u00ednicos, Hospital Universitari Arnau de Vilanova de Lleida, Institut de Recerca Biom\u00e8dica de Lleida (IRB Lleida), Universitat de Lleida , Lleida ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"G\u00f3mez-Arbon\u00e9s","sequence":"additional","affiliation":[{"name":"Departamento de Medicina y Cirug\u00eda, Institut de Recerca Biom\u00e8dica de Lleida (IRB Lleida), Universitat de Lleida , Lleida ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Cort\u00e9s-Lara","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Univeristario Son Espases, Instituto de Investigaci\u00f3n Sanitaria Illes Balears, CIBERINFEC , Palma de Mallorca ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merc\u00e8","family":"Garc\u00eda-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Servicio de An\u00e1lisis Cl\u00ednicos, Hospital Universitari Arnau de Vilanova de Lleida, Institut de Recerca Biom\u00e8dica de Lleida (IRB Lleida), Universitat de Lleida , Lleida ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Pifarr\u00e9-Teixid\u00f3","sequence":"additional","affiliation":[{"name":"Servicio de Neumolog\u00eda, Hospital Universitari Arnau de Vilanova de Lleida , Lleida ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Oliver","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Univeristario Son Espases, Instituto de Investigaci\u00f3n Sanitaria Illes Balears, CIBERINFEC , Palma de Mallorca ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"key":"2025040812103616700_dlaf053-B1","doi-asserted-by":"publisher","first-page":"685","DOI":"10.3389\/fmicb.2018.00685","article-title":"The versatile 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