{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T03:17:22Z","timestamp":1774927042456,"version":"3.50.1"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":15,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Sepsis is a medical emergency and rapid antimicrobial susceptibility testing (RAST) is essential for patient management. However, existing RAST protocols may be unsuitable for resource-limited settings due to the need for rapid species identification, which may require specialized equipment or expensive reagents.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>To minimize the requirement of the RAST protocol while maintaining its efficacy, focusing on Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii and Staphylococcus aureus.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Positive blood cultures suspected of having these pathogens underwent RAST with three main modifications: delayed species identification, implementation of pan-species breakpoints and the change in the quality control process. Twelve antimicrobials were tested for Gram-negative bacilli, and three for S. aureus. Species identification was performed by both the MALDI-TOF MS and the rapid phenotypic tests at the final RAST time point. The categorical agreement was evaluated against the standard AST method and the RAST protocol.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Among 398 samples, gentamicin, ampicillin, meropenem and trimethoprim-sulfamethoxazole met the accuracy criteria for Gram-negative bacilli. Ceftriaxone, imipenem and ciprofloxacin had slightly reduced agreement (80%\u201390%) due to a high false resistance. The remaining antimicrobials either had a low agreement or high false susceptibility. Only gentamicin passed the agreement criteria for S. aureus. The use of pan-species breakpoints resulted in several failed results without improvement in the concordance. The quality control process with and without sheep blood yielded comparable results.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>RAST reduced the time-to-result for key antimicrobial agents for at least 24\u2005h while requiring minimal workflow disruption, enabling early adjustment of antimicrobial treatment.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jacamr\/dlaf079","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T10:11:28Z","timestamp":1747217488000},"source":"Crossref","is-referenced-by-count":1,"title":["Modification of the rapid antimicrobial susceptibility testing from blood culture protocol for a resource-limited setting"],"prefix":"10.1093","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2538-9775","authenticated-orcid":false,"given":"Korakrit","family":"Imwattana","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok ,","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Usa","family":"Kijsinthopchai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok ,","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samaporn","family":"Yongyod","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok 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