{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T06:17:02Z","timestamp":1772605022780,"version":"3.50.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":28,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["L232071"],"award-info":[{"award-number":["L232071"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["7232007"],"award-info":[{"award-number":["7232007"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["7242015"],"award-info":[{"award-number":["7242015"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing High-Level Public Health Technical Talent Project","award":["2023-02-08"],"award-info":[{"award-number":["2023-02-08"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170201"],"award-info":[{"award-number":["32170201"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Municipal Public Welfare Development and Reform Pilot Project for Medical Research Institutes","award":["JYY2023-10"],"award-info":[{"award-number":["JYY2023-10"]}]},{"name":"Beijing Hospitals Authority's Ascent Plan","award":["DFL20241301"],"award-info":[{"award-number":["DFL20241301"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To analyse the in vitro antimicrobial susceptibility of Mycoplasma pneumoniae (M. pneumoniae) isolates from 10 different provinces\/municipalities across China during the 2023 epidemic.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We collected respiratory specimens from paediatric patients diagnosed with M. pneumoniae pneumonia from 10 different provinces\/municipalities including Beijing, Shanghai, Liaoning, Gansu, Shanxi, Henan, Hunan, Jiangsu, Guangxi and Guangdong across China from October to December 2023. M. pneumoniae was isolated and cultured from these specimens. Macrolide resistance-associated mutations A2063G or A2064G in the 23S rRNA gene were detected. Minimum inhibitory concentrations (MICs) of five antibiotics (erythromycin, azithromycin, tetracycline, moxifloxacin and doxycycline) were determined using PPLO broth and microdilution susceptibility tests.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>A total of 190 M. pneumoniae isolates were analysed. All isolates harboured the A2063G mutation. All isolates (100%) were highly resistant to erythromycin (MIC range: 128\u20131024\u2005mg\/L) and azithromycin (MIC range: 4\u2013256\u2005mg\/L), but susceptible to tetracycline (MIC range: \u22640.125\u20131\u2005mg\/L), doxycycline (MIC range: \u22640.125\u20131\u2005mg\/L) and moxifloxacin (MIC range: \u22640.125\u2005mg\/L). For azithromycin and tetracycline, M. pneumoniae isolates from different regions of China showed different susceptibilities. The MICs of tetracycline against M. pneumoniae from northern China were significantly lower than those from central China and southern China. Comparisons among provinces\/municipalities suggested that isolates from Hunan and Beijing were more resistant to azithromycin. For tetracycline, the MICs were significantly lower among isolates from Beijing.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>During the 2023 epidemic, isolates of M. pneumoniae were highly resistant to macrolides but remained susceptible to tetracycline and quinolones. For the treatment of macrolide-resistant M. pneumoniae, tetracycline, moxifloxacin and doxycycline would be the recommended antibiotics based on our findings.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jacamr\/dlaf124","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T07:25:41Z","timestamp":1751268341000},"source":"Crossref","is-referenced-by-count":2,"title":["<i>In vitro<\/i> antimicrobial susceptibility of <i>Mycoplasma pneumoniae<\/i> isolates across different regions of China in 2023"],"prefix":"10.1093","volume":"7","author":[{"given":"Chao","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]},{"name":"Capital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyu","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]},{"name":"Department of Allergy, Capital Center for Children\u2019s Health, Capital Medical University, Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanqing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanling","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guanhua","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinghua","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuanfeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics , Beijing ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"2025073004001449700_dlaf124-B1","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1007\/s12519-024-00831-0","article-title":"Expert consensus on the diagnosis and treatment of macrolide-resistant Mycoplasma pneumoniae pneumonia in children","volume":"20","author":"Wang","year":"2024","journal-title":"World J 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