{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:31:24Z","timestamp":1792704684120,"version":"4.5.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":50,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["112-2314-B-002-143-MY3"],"award-info":[{"award-number":["112-2314-B-002-143-MY3"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Invasive fusariosis is rising and poses challenges due to species complexity and antifungal resistance. In vitro susceptibilities of new antifungals against Fusarium isolates are seldomly evaluated in Asia. This study aimed to evaluate a two-step molecular identification algorithm and to perform in vitro antifungal susceptibility with correlation of species and susceptibility patterns.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fusarium clinical isolates collected at three hospitals in Taiwan (2011\u20132023) were identified to species level using sequential ITS and TEF1\u03b1 sequencing (step I), followed by RBP2 sequencing (step II) for inconclusive isolates. Minimum effective\/inhibitory concentrations (MECs\/MICs) of manogepix, olorofim, amphotericin B and voriconazole were determined by EUCAST method (E.Def 9.4).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of 103 isolates (37 blood and 66 cornea isolates) evaluated, the two-step algorithm achieved &amp;gt;90% to species level. Fusarium solani species complex (FSSC) was predominant, especially in blood isolates (86.5% versus 65.2% in cornea isolates; P\u200a=\u200a0.02). The rest 28 isolates belonged to 12 species within six species complexes (SCs). Manogepix exhibited potent activity against all isolates (MEC \u22640.015\u2005mg\/L), while olorofim activities varied by SCs, with MIC \u22640.25\u2005mg\/L against Fusarium fujikuroi SC. FSSC displayed higher voriconazole and amphotericin B MICs compared with other SCs, with Neocosmospora keratoplastica displaying a highest amphotericin B modal MIC of 4\u2005mg\/L. Four major Neocosmospora species showed voriconazole MIC \u226516\u2005mg\/L.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings indicated the two-step molecular algorithm accurately identifies Fusarium to species level. Further, we underscored the significance of considering both Fusarium SCs and species for predicting antifungal susceptibility, particularly to olorofim and amphotericin B.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jacamr\/dlag022","type":"journal-article","created":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T12:38:37Z","timestamp":1770467917000},"source":"Crossref","is-referenced-by-count":2,"title":["Assessment of the molecular identification algorithm and its impact on antifungal susceptibilities against clinical\n                    <i>Fusarium<\/i>\n                    isolates: a multicentre study in Taiwan, 2011\u20132023"],"prefix":"10.1093","volume":"8","author":[{"given":"Pao-Yu","family":"Chen","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, National Taiwan University Hospital , Taipei 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