{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T10:06:30Z","timestamp":1779185190958,"version":"3.51.4"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":19,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006489","name":"French Alternative Energies and Atomic Energy Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006489","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007241","name":"University of Paris-Saclay","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002738","name":"AP-HP","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>ESBL-producing Enterobacterales are a major cause of bloodstream infections, requiring rapid detection for timely antimicrobial therapy. We evaluated the performance of the NG-TEST\u00ae CTX-M MULTI lateral flow immunoassay combined with the NG-TEST\u00ae Blood Culture Prep (BCP) Kit for detecting CTX-M ESBLs directly from positive blood cultures.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In total 165 clinical Enterobacterales were used to spike blood culture bottles. These included 32 non\u2013CTX-M producers, 120 CTX-M producers belonging to the five main CTX-M groups, and 13 Kluyvera sp. Blood cultures were placed in the BACT\/ALERT\u00ae VIRTUO\u00ae system (bioM\u00e9rieux); upon bacterial growth detection by the system a UriSelect 4 Agar (Bio-Rad) was inoculated to verify purity. Bacteria were prepared using the NG-TEST\u00ae BCP Kit followed by the NG-TEST\u00ae CTX-M MULTI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The NG-TEST\u00ae CTX-M MULTI detected all 120 CTX-M\u2013producing isolates expressing CTX-M variants belonging to the five groups (1, 2, 8, 9 and 25) from both colonies and blood cultures, resulting in 100% sensitivity. The NG-TEST\u00ae BCP Kit removed all the patients\u2019 blood cells and reliably extracted bacteria irrespective of the bacterial species. Non\u2013CTX-M producers gave negative results, confirming 100% specificity. Among CTX-M\u2013producing Kluyvera species, eight isolates tested positive, while five were repeatedly negative, and consistent with prior reports.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our results demonstrate that the combination of the NG-TEST\u00ae BCP Kit and the NG-TEST\u00ae CTX-M MULTI systems provides a rapid (&amp;lt;20\u2005min), user-friendly, robust and accurate tool for species-independent detection of CTX-M\u2013type ESBLs directly from positive blood cultures, supporting earlier targeted antimicrobial therapy in bloodstream infections.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jacamr\/dlag080","type":"journal-article","created":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T12:12:21Z","timestamp":1777637541000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the NG-TEST\u00ae blood culture PREP kit for ESBL directly from positive blood cultures using the NG-TEST\u00ae CTX-M MULTI lateral flow immunoassay"],"prefix":"10.1093","volume":"8","author":[{"given":"Saoussen","family":"Oueslati","sequence":"first","affiliation":[{"name":"Team \u2018Resist\u2019, UMR1184 \u2018Infectious Disease Models and Innovative Therapies (IDMIT)\u2019 INSERM, Universit\u00e9 Paris-Saclay, CEA, Healthi , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Department of Bacteriology-Hygiene, Bic\u00eatre Hospital, APHP Paris-Saclay , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Essonne (\u00cele-de-France), SEPSIS Comprehensive Center \u2013 IHU SEPSIS , Gif-sur-Yvette ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niroshika","family":"Visvanathan","sequence":"additional","affiliation":[{"name":"Team \u2018Resist\u2019, UMR1184 \u2018Infectious Disease Models and Innovative Therapies (IDMIT)\u2019 INSERM, Universit\u00e9 Paris-Saclay, CEA, Healthi , Le Kremlin-Bic\u00eatre ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Orlandi","sequence":"additional","affiliation":[{"name":"Team \u2018Resist\u2019, UMR1184 \u2018Infectious Disease Models and Innovative Therapies (IDMIT)\u2019 INSERM, Universit\u00e9 Paris-Saclay, CEA, Healthi , Le Kremlin-Bic\u00eatre ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Blandine","family":"Baumy","sequence":"additional","affiliation":[{"name":"Research and Development Department, NG Biotech , Guipry ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Francius","sequence":"additional","affiliation":[{"name":"Research and Development Department, NG Biotech , Guipry ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herv\u00e9","family":"Volland","sequence":"additional","affiliation":[{"name":"D\u00e9partement M\u00e9dicaments et Technologies Pour la Sant\u00e9, Universit\u00e9 Paris-Saclay, CEA, INRAE , Gif-sur-Yvette 91191 ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnaud","family":"Chalin","sequence":"additional","affiliation":[{"name":"Research and Development Department, NG Biotech , Guipry ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9937-9572","authenticated-orcid":false,"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"Team \u2018Resist\u2019, UMR1184 \u2018Infectious Disease Models and Innovative Therapies (IDMIT)\u2019 INSERM, Universit\u00e9 Paris-Saclay, CEA, Healthi , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Department of Bacteriology-Hygiene, Bic\u00eatre Hospital, APHP Paris-Saclay , Le Kremlin-Bic\u00eatre ,","place":["France"]},{"name":"Essonne (\u00cele-de-France), SEPSIS Comprehensive Center \u2013 IHU SEPSIS , Gif-sur-Yvette 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