{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T07:25:23Z","timestamp":1780730723077,"version":"3.54.1"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T00:00:00Z","timestamp":1595808000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>While molecular techniques remain the gold standard for diagnosis of acute SARS-CoV-2 infection, serological tests have the unique potential to ascertain how much of the population has been exposed to the COVID-19 pathogen. There have been limited published studies to date documenting the performance of SARS-CoV-2 antibody assays.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We compared the DiaSorin Liaison SARS-CoV-2 S1\/S2 IgG and Roche Diagnostics Elecsys Anti-SARS-CoV-2 assays using 228 samples spanning patients with positive PCR for SARS-CoV-2, patients with compatible symptoms but negative PCR, pre-COVID specimens, and potential cross-reactives.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Both assays detected antibodies in 18\/19 samples collected at least one week after a positive PCR result. Neither method consistently detected antibodies in specimens collected within one week of a positive PCR result (sensitivity &amp;lt; 50%), but antibodies were detected by only Roche in four samples in this time frame. Using 139 pre-COVID and 35 PCR-negative samples, the Roche and DiaSorin assays demonstrated specificities of 100.0% and 98.9%, respectively. Neither assay demonstrated cross-reactivity from other coronaviruses (229E, HKU1, NL63, OC43), respiratory pathogens (adenovirus, metapneumovirus, rhinovirus\/enterovirus), or antibodies to other viruses (HIV, EBV, CMV, HBV, HCV, HAV).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Discussion<\/jats:title>\n                  <jats:p>Overall, the qualitative interpretations afforded by the Roche and DiaSorin assays agreed for 97% of samples evaluated. Minor discrepancies in sensitivity and specificity were observed between methods, with the differences in specificity more clinically significant for our low-prevalence population. For the DiaSorin assay, all disagreements with the Roche assay occurred in samples with quantitative signals near the cut-off determining positivity.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jalm\/jfaa125","type":"journal-article","created":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T03:49:51Z","timestamp":1595303391000},"page":"1351-1357","source":"Crossref","is-referenced-by-count":33,"title":["Head-to-Head Comparison of Two SARS-CoV-2 Serology Assays"],"prefix":"10.1093","volume":"5","author":[{"given":"Anna E","family":"Merrill","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J Brooks","family":"Jackson","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Ehlers","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dena","family":"Voss","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew D","family":"Krasowski","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,7,27]]},"reference":[{"key":"2020110800224456500_jfaa125-B1","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1093\/clinchem\/hvaa029","article-title":"Molecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia","volume":"66","author":"Chu","year":"2020","journal-title":"Clin Chem"},{"key":"2020110800224456500_jfaa125-B2","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","article-title":"Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR","volume":"25","author":"Corman","year":"2020","journal-title":"Euro Surveill"},{"key":"2020110800224456500_jfaa125-B3"},{"key":"2020110800224456500_jfaa125-B4","year":"2019"},{"key":"2020110800224456500_jfaa125-B5"},{"key":"2020110800224456500_jfaa125-B6","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/s41591-020-0897-1","article-title":"Antibody responses to SARS-CoV-2 in patients with COVID-19","volume":"26","author":"Long","year":"2020","journal-title":"Nat Med"},{"key":"2020110800224456500_jfaa125-B7","first-page":"26","article-title":"Severe acute respiratory syndrome coronavirus 2-specific antibody responses in coronavirus disease 2019 patients","author":"Okba","year":"2020","journal-title":"Emerg Infect Dis"},{"key":"2020110800224456500_jfaa125-B8","author":"Whitman","year":"2020"},{"key":"2020110800224456500_jfaa125-B9","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00941-20","article-title":"Performance characteristics of the Abbott architect SARS-CoV-2 IgG assay and seroprevalence in Boise, Idaho","author":"Bryan","year":"2020","journal-title":"J Clin Microbiol"},{"key":"2020110800224456500_jfaa125-B10","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01243-20","article-title":"Performance characteristics of four high-throughput immunoassays for detection of IgG antibodies against SARS-CoV-2","author":"Theel","year":"2020","journal-title":"J Clin Microbiol"},{"key":"2020110800224456500_jfaa125-B11","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1093\/clinchem\/hvaa120","article-title":"Clinical performance of two SARS-COV-2 serologic assays","volume":"66","author":"Tang","year":"2020","journal-title":"Clin Chem"},{"key":"2020110800224456500_jfaa125-B12","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1093\/clinchem\/hvaa132","article-title":"Clinical performance of the Roche SARS-CoV-2 serologic assay","volume":"66","author":"Tang","year":"2020","journal-title":"Clin Chem"},{"key":"2020110800224456500_jfaa125-B13","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1093\/clinchem\/hvaa107","article-title":"SARS-CoV-2 serology: much hype, little data","volume":"66","author":"Farnsworth","year":"2020","journal-title":"Clin Chem"},{"key":"2020110800224456500_jfaa125-B14","doi-asserted-by":"crossref","DOI":"10.1093\/jalm\/jfaa079","article-title":"Longitudinal monitoring of SARS-CoV-2 IgM and IgG seropositivity to detect COVID-19","volume":"5","author":"Suhandynata","year":"2020","journal-title":"J Appl Lab Med"},{"key":"2020110800224456500_jfaa125-B15","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1093\/labmed\/lmaa025","article-title":"Double-edged spike-are SARS-CoV-2 serologic tests safe right now?","volume":"51","author":"Torres","year":"2020","journal-title":"Lab Med"},{"key":"2020110800224456500_jfaa125-B16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/ajcp\/aqaa082","article-title":"Antibody testing for COVID-19","volume":"154","author":"Mathur","year":"2020","journal-title":"Am J Clin Pathol"},{"key":"2020110800224456500_jfaa125-B17"}],"container-title":["The Journal of Applied Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jalm\/advance-article-pdf\/doi\/10.1093\/jalm\/jfaa125\/33718810\/jfaa125.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jalm\/article-pdf\/5\/6\/1351\/34161151\/jfaa125.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jalm\/article-pdf\/5\/6\/1351\/34161151\/jfaa125.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,8]],"date-time":"2020-11-08T05:23:08Z","timestamp":1604812988000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jalm\/article\/5\/6\/1351\/5876836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,27]]},"references-count":17,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,7,27]]},"published-print":{"date-parts":[[2020,11,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jalm\/jfaa125","relation":{},"ISSN":["2576-9456","2475-7241"],"issn-type":[{"value":"2576-9456","type":"print"},{"value":"2475-7241","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,11]]},"published":{"date-parts":[[2020,7,27]]}}}