{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T06:00:53Z","timestamp":1757311253971,"version":"3.37.3"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,11,29]],"date-time":"2020-11-29T00:00:00Z","timestamp":1606608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"NIAMS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Roche Diagnostics to institution"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Matched serum and urine samples from patients who had total hip replacement were used to assess serum-validated immunoassay reagents for use in urine.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Samples were evaluated by an automated electrochemiluminescent immunoassay (cobas e411; Roche Diagnostics) for C-terminal telopeptide of type I collagen isoform \u03b2 (\u03b2-Crosslaps), osteocalcin N-terminal midfragment (N-MID OC), N-terminal propeptide of type I collagen (PINP), and interleukin 6 (IL-6). Spike and recovery experiments were utilized to assess urinary matrix effects. Correlations between serum and both raw and creatinine-corrected urinary measures were assessed. Short-term precision was assessed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Spike and recovery experiments indicated minimal matrix effects of urine for the \u03b2-Crosslaps assay. Potential matrix effects were observed for the other analytes because N-MID OC and IL-6 tended to be slightly overrecovered, whereas PINP was underrecovered. There were strong correlations between serum \u03b2-Crosslaps and raw (Spearman \u03c1 [rs] = 0.725, P &amp;lt; 0.0001) and creatinine-corrected (rs = 0.793, P &amp;lt; 0.0001) urinary measures and moderate correlations between serum N-MID OC and raw (rs = 0.582, P &amp;lt; 0.0001) and creatinine-corrected (rs = 0.482, P &amp;lt; 0.0001) urinary measures. PINP was not detected in urine, and no significant serum\u2013urine correlations were found for IL-6. Short-term precision for urinary levels of \u03b2-Crosslaps, N-MID OC, and IL-6 were 1.6%, 6.3% and 14.4%, respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Urinary measurements of \u03b2-Crosslaps and N-MID OC assays were correlated with serum measurements and had good short-term precision. Urinary PINP was not detectable. IL-6 can be measured in urine using this technology, but the levels did not correlate with serum levels, and the short-term precision was variable.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jalm\/jfaa172","type":"journal-article","created":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T03:25:28Z","timestamp":1599967528000},"page":"750-755","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of Bone Turnover Biomarkers in Serum and Urine Measured on an Automated Analytical Platform"],"prefix":"10.1093","volume":"6","author":[{"given":"Brittany M","family":"Wilson","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Medicine, Rush University Medical Center, Chicago, IL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan D","family":"Ross","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Medicine, Rush University Medical Center, Chicago, IL"},{"name":"Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua J","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dale Rick","family":"Sumner","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Medicine, Rush University Medical Center, Chicago, IL"},{"name":"Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,11,29]]},"reference":[{"key":"2021043008010791500_jfaa172-B1","doi-asserted-by":"crossref","first-page":"2754","DOI":"10.1002\/jor.24044","article-title":"Discovery of biomarkers to identify peri-implant osteolysis before radiographic 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