{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T19:49:19Z","timestamp":1770061759874,"version":"3.49.0"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T00:00:00Z","timestamp":1606867200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/www.nationalarchives.gov.uk\/doc\/open-government-licence\/version\/3\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Aldosterone and renin are pivotal hormones in the regulation of salt and water homeostasis and blood pressure. Measurement of renin and aldosterone in serum\/plasma is essential for the investigation of primary hyperaldosteronism (PA) and monitoring of glucocorticoid replacement therapy.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We report 2 LC-MS\/MS methods developed to measure aldosterone and plasma renin activity (PRA). PRA was determined by endogenous enzymatic generation of angiotensin I using 150\u2009\u00b5L of sample. Generated angiotensin I was purified by solid phase extraction prior to chromatographic separation and mass spectrometry. Aldosterone measurement required 300\u2009\u03bcL of sample extracted with MTBE prior to LC-MS\/MS analysis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The PRA method was linear (1.2\u2013193 nmol\/L), sensitive (LLOQ = 1.2\u2009nmol\/L), precise (CV = 4.1%), and specific (no cross reactivity for a number of structurally similar steroids). Dilutional linearity and recovery (84%) were acceptable. Accuracy was confirmed by comparison against our current RIA method. The aldosterone method had equally acceptable performance characteristics. Reference ranges in 110 healthy normotensive subjects were: PRA 0.2\u20133.7\u2009nmol\/L\/h and aldosterone 50\u2013950 pmol\/L. Consecutive patients (n\u2009=\u200962) with adrenal incidentalomas shown to have no functional adrenal disease; their post overnight 1\u2009mg dexamethasone test values were: PRA 0.2\u20132.6\u2009nmol\/L\/h and aldosterone 55\u2013480 pmol\/L. Serum aldosterone values after 2\u2009liter saline suppression were\u2014normal subjects (n\u2009=\u200917): 78\u2013238 pmol\/L and confirmed primary hyperaldosteronism (n\u2009=\u200925): 131\u20131080 pmol\/L.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We have developed robust assays for PRA and aldosterone with appropriate clinical evaluation. These assays are now in routine practice in the UK.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jalm\/jfaa177","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T19:14:42Z","timestamp":1602011682000},"page":"668-678","source":"Crossref","is-referenced-by-count":9,"title":["Clinical Evaluation of Assays for Plasma Renin Activity and Aldosterone Measurement by Liquid Chromatography\u2013Tandem Mass Spectrometry"],"prefix":"10.1093","volume":"6","author":[{"given":"Stephen M","family":"Gibbons","sequence":"first","affiliation":[{"name":"SAS Laboratory Endocrinology, St James University Hospital, Leeds, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen P","family":"Field","sequence":"additional","affiliation":[{"name":"SAS Laboratory Endocrinology, St James University Hospital, Leeds, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"April","family":"Fairhurst","sequence":"additional","affiliation":[{"name":"SAS Laboratory Endocrinology, St James University Hospital, Leeds, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Fleming","sequence":"additional","affiliation":[{"name":"SAS Laboratory Endocrinology, St James University Hospital, Leeds, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clive","family":"Ford","sequence":"additional","affiliation":[{"name":"SAS Laboratory Endocrinology, St James University Hospital, Leeds, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma L","family":"Williams","sequence":"additional","affiliation":[{"name":"SAS Laboratory Endocrinology (Part of North West London Pathology), Charing Cross Hospital, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie C","family":"Barnes","sequence":"additional","affiliation":[{"name":"SAS Laboratory Endocrinology (Part of North West London Pathology), Charing Cross Hospital, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian H","family":"Barth","sequence":"additional","affiliation":[{"name":"SAS Laboratory Endocrinology, St James University Hospital, Leeds, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,11,30]]},"reference":[{"key":"2021043007581243500_jfaa177-B1","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1007\/BF01468900","article-title":"Improvement of renin determination in human plasma using a commonly available renin standard in a radioimmunological method","volume":"53","author":"Hummerich","year":"1975","journal-title":"Klin 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