{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T12:20:02Z","timestamp":1763986802786},"reference-count":4,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T00:00:00Z","timestamp":1618963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Based on guidelines from the Infectious Diseases Society of America and the American Society for Microbiology, many pediatric hospitals are implementing weight-based collection guidelines for blood cultures. To simplify the process of culture collection, there has been interest in validating the use of adult blood culture bottles with low volumes, which may allow for a \u201cone size fits all\u201d bottle.<\/jats:p>\n <\/jats:sec>\n \n Method<\/jats:title>\n This study examined 9 clinically relevant organisms (Staphylococcus aureus, Streptococcus pneumonia, Streptococcus agalactiae, Enterococcus faecium, Escherichia coli, Haemophilus influenzae, Pseudomonas aeruginosa, Candida glabrata, and Candida albicans) utilizing the BD BacTec system at various inoculation volumes and dilutions to assess performance, based on time to positivity, of adult blood culture bottles compared with pediatric blood culture bottles.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n There was a lack of detection of H. influenzae using adult blood culture bottles inoculated at low volumes, whereas pediatric bottles detected H. influenzae regardless of dilution\u2013volume combinations tested.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Exclusive use of adult blood culture bottles may not detect H. influenzae bacteremia in the setting of low-volume inoculum.<\/jats:p>\n <\/jats:sec>","DOI":"10.1093\/jalm\/jfab013","type":"journal-article","created":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T20:14:04Z","timestamp":1614629644000},"page":"1601-1606","source":"Crossref","is-referenced-by-count":2,"title":["Lack of Detection of Haemophilus influenzae<\/i> Using Adult Blood Culture Bottles Inoculated at Low Volumes"],"prefix":"10.1093","volume":"6","author":[{"given":"Tanner J","family":"Freeman","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlene","family":"Krenke","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, UPMC Children\u2019s Hospital of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie L","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA"},{"name":"Department of Pathology and Laboratory Medicine, UPMC Children\u2019s Hospital of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,4,21]]},"reference":[{"key":"2021110213153166300_jfab013-B1","doi-asserted-by":"crossref","first-page":"e1\u201394","DOI":"10.1093\/cid\/ciy584","article-title":"A guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2018 update by the Infectious Diseases Society of America and the American Society for Microbiology","volume":"67","author":"Miller","year":"2018","journal-title":"Clin Infect Dis"},{"key":"2021110213153166300_jfab013-B2","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.1128\/JCM.01706-15","article-title":"Blood volume required for detection of low levels and ultralow levels of organisms responsible for neonatal bacteremia by use of BacTec Peds Plus\/F, Plus Aerobic\/F Medium, and the BD BacTec FX system: an in vitro study","volume":"53","author":"Lancaster","year":"2015","journal-title":"J Clin Microbiol"},{"key":"2021110213153166300_jfab013-B3","doi-asserted-by":"crossref","first-page":"3728","DOI":"10.1128\/JCM.05476-11","article-title":"Haemophilus influenzae infections in the Haemophilus influenzae type B conjugate vaccine era","volume":"49","author":"Agrawal","year":"2011","journal-title":"J Clin Microbiol"},{"key":"2021110213153166300_jfab013-B4","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1128\/JCM.02919-15","article-title":"Diagnosis of bloodstream infections in children","volume":"54","author":"Bard","year":"2016","journal-title":"J Clin Microbiol"}],"container-title":["The Journal of Applied Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jalm\/article-pdf\/6\/6\/1601\/41046831\/jfab013.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jalm\/article-pdf\/6\/6\/1601\/41046831\/jfab013.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T13:27:30Z","timestamp":1635859650000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jalm\/article\/6\/6\/1601\/6242674"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,21]]},"references-count":4,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,4,21]]},"published-print":{"date-parts":[[2021,11,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jalm\/jfab013","relation":{},"ISSN":["2576-9456","2475-7241"],"issn-type":[{"value":"2576-9456","type":"print"},{"value":"2475-7241","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,11,1]]},"published":{"date-parts":[[2021,4,21]]}}}