{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:24:08Z","timestamp":1787009048941,"version":"build-2736575974"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T00:00:00Z","timestamp":1624320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100016545","name":"Roche Diagnostics","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016545","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The ability to rapidly detect severe accurate respiratory syndrome coronavirus virus-2 (SARS-CoV-2) and influenza virus infection is vital for patient care due to overlap in clinical symptoms. Roche\u2019s cobas\u00ae Liat\u00ae SARS-CoV-2 &amp; Influenza A\/B Nucleic Acid Test used on the cobas Liat was granted approval under the Food and Drug\u2019s Emergency Use Authorization for nasopharyngeal (NP) and nasal swabs collected in viral\/universal transport medium (VTM\/UTM). However, there is a critical need for media that inactivates the virus, especially when specimens are collected in decentralized settings. This study aimed to investigate the use of PrimeStore Molecular Transport Medium\u00ae (PS-MTM\u00ae), designed to inactivate\/kill and stabilize RNA\/DNA for ambient transport and preprocessing of collected samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A limit of detection (LOD) using serially diluted SARS-CoV-2 RNA in PS-MTM and routine UTM was established using standard quantitative PCR (qPCR). Additionally, a clinical panel of NP and oral swabs collected in PS-MTM during the 2020 coronavirus disease 2019 pandemic were evaluated on the cobas Liat and compared to \u201cgold standard\u201d qPCR on an ABI-7500 instrument.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>SARS-CoV-2 RNA LOD using standard qPCR was equivalent on the cobas Liat instrument. cobas Liat detection from oral\/NP swabs in PS-MTM media exhibited equivalent positive percent agreement (100%) and negative percent agreement (96.4%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PS-MTM and the Roche cobas Liat are compatible and complimentary devices for respiratory specimen collection and rapid disease detection, respectively. PS-MTM is equivalent to standard VTM\/UTM with the added benefit of safe, noninfectious sample processing for near-patient testing.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jalm\/jfab073","type":"journal-article","created":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T15:12:51Z","timestamp":1624029171000},"page":"1409-1416","source":"Crossref","is-referenced-by-count":12,"title":["Rapid and Safe Detection of SARS-CoV-2 and Influenza Virus RNA Using Onsite Quantitative PCR Diagnostic Testing From Clinical Specimens Collected in Molecular Transport Medium"],"prefix":"10.1093","volume":"6","author":[{"given":"Luke T","family":"Daum","sequence":"first","affiliation":[{"name":"Longhorn Vaccines and Diagnostics, San Antonio, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald W","family":"Fischer","sequence":"additional","affiliation":[{"name":"Longhorn Vaccines and Diagnostics, San Antonio, TX, 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